Oxidative stress in corneal tissue in experimental keratitis due to Aspergillus flavus: Effect of topical voriconazole therapy

Ruban, Vasanthakumar Vasantha; Anbukkarasi, Muniyandi; Anand, T.; Thomas, Philip A.; Geraldine, Pitchairaj

doi:10.1016/j.bcab.2019.101323

Cited by 3 publications

(4 citation statements)

References 37 publications

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“…MDA elevation is a good indicator for lipid peroxidation (27). In ulcerative keratitis, the elevation in MDA and reduction in CAT and superoxide dismutase (SOD) were recorded and attributed to inflammation (62).…”

Section: Corneal Ulcer and Oxidative Stressmentioning

confidence: 99%

“…TAC could be a simple tool to assess antioxidant systems regardless of system antioxidant modus operandi (66). Experimental keratitis was associated with oxidative stress in the cornea (62). Oxidative stress in corneal affections is linked to many factors, and this process can disrupt the cornea, which ranged from a reduction in visual perception to vision loss (58).…”

Section: Corneal Ulcer and Oxidative Stressmentioning

confidence: 99%

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Corneal Ulcer in Dogs and Cats: Novel Clinical Application of Regenerative Therapy Using Subconjunctival Injection of Autologous Platelet-Rich Plasma

et al. 2021

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Background: Corneal ulcer could be a major source of distress in small animals, with many contributing agents. In recent years, few studies evaluated the efficacy of platelet-rich plasma (PRP) in healing corneal ulcers.Aim: This study aimed to assess the ability of subconjunctival injection of autologous PRP in the treatment of corneal ulcers in dogs and cats as well as estimate the expression of matrix metalloproteinase (MMP)-2, MMP-9, and oxidative stress biomarkers in these patients.Methods: A total number of 28 animals (16 cats and 12 dogs) were enrolled in this study. Each animal was subjected to clinical, neurologic, and ophthalmic examinations where the type of ulcer was documented. Tear samples were collected for evaluation of oxidative biomarkers and MMPs; conjunctival swabs were taken to identify the involved organism. PRP was prepared from each animal and given as subconjunctival injection; numbers of injections were done according to case response. Clinical follow-up was done and documented for each case.Results: In cat patients, female and Persian cats were most affected; unilateral and superficial ulcers were most recorded. In male dogs, unilateral, and superficial ulcers were most recorded. FHV-1 was most identified in cats, while Staphylococcus aureus was most identified in dogs. Numbers of injections needed to achieve healing were recorded, with 50% of dogs needing two injections with 1-week intervals and 50% of cats needed three injections with 1-week intervals. Alterations in both oxidative biomarkers and MMPs were recorded in affected animals.Conclusion: The use of autologous PRP as a subconjunctival injection in treating corneal ulcers in dogs and cats is effective. The number of injections is the case and corneal ulcer type-dependent.Clinical Significance: Autologous PRP as a subconjunctival injection in treating corneal ulcer is a relatively cheap, safe method and can be done in the clinical setting.

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Section: Corneal Ulcer and Oxidative Stressmentioning

confidence: 99%

Section: Corneal Ulcer and Oxidative Stressmentioning

confidence: 99%

Corneal Ulcer in Dogs and Cats: Novel Clinical Application of Regenerative Therapy Using Subconjunctival Injection of Autologous Platelet-Rich Plasma

et al. 2021

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“…Abnormal oxidative stress behavior in the form of reduced SOD and CAT and elevated MDA were also observed in the conjunctival and corneal tissues of mice and rabbits injured by ultraviolet light and fungal keratitis ( Aspergillus flavus ) and in the tears of dogs and cats with different types of corneal ulcers 25–28,45 . In these studies, subjects treated with a supposed antioxidant medication showed higher levels of SOD and CAT and lower levels of MDA 25–28,45 . Another in vitro study demonstrated that the levels of superoxide anions were higher in conjunctival epithelial cell lines of humans treated with PAGs containing BAC than in those treated with a formulation of PAG without BAC 46 .…”

Section: Discussionmentioning

confidence: 94%

“…[25][26][27][28]45 In these studies, subjects treated with a supposed antioxidant medication showed higher levels of SOD and CAT and lower levels of MDA. [25][26][27][28]45 Another in vitro study demonstrated that the levels of superoxide anions were higher in conjunctival epithelial cell lines of humans treated with PAGs containing BAC than in those treated with a formulation of PAG without BAC. 46 The higher levels of MDA observed in the eyes that received BACKT at least suggest that BAC stimulates higher lipid peroxidation and, therefore, still plays a detrimental role in the conjunctival tissue, despite the absence of statistical significance between treatments found in the clinical parameters assessed by OSAvet® and the GCD assessed by histology.…”

Section: Baselinementioning

confidence: 91%

Effects of topical ketorolac tromethamine on tear parameters, meibography, goblet cell density, and conjunctival oxidative stress in healthy dogs

Sonego,

Ribeiro,

Dower

et al. 2023

Veterinary Ophthalmology

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ObjectivesThe objective of the study was to evaluate whether a twice‐daily instillation of 0.45% preservative‐free ketorolac tromethamine (FKT) or 0.4% benzalkonium chloride‐preserved ketorolac tromethamine (BACKT), every 12 h for 30 days may affect tear film parameters and the meibography in healthy dogs. Additionally, we assessed whether the same treatments irritated the ocular surface, affected goblet cell density (GCD), and the levels of oxidative stress biomarkers (OSB) in the conjunctiva of the same dogs.ProceduresExperimental and masked comparison study. In 11 healthy dogs baseline values of the lipid layer thickness, tear meniscus height, non‐invasive tear breakup time (NI‐TFBT), and the meibomian gland (MG) loss were assessed by OSAvet®. For each dog, one eye received 40 μL of BACKT, while the other received 40 μL FKT, every 12 h for 30 consecutive days. Tear parameters and meibography were repeated 15, 30, and 60 days post‐treatments. Conjunctival hyperemia and blepharospasm were monitored at the same time points. At baseline and Day 30, a conjunctival biopsy was collected for GCD and OSB determination.ResultsConjunctival hyperemia and blepharospasm were not observed. At Day 15, the MG loss increased only in FKT‐treated eyes (p < .001). On Day 30, both treatment groups showed increased MG loss, shortened NI‐TFBT, and reduced GCD and catalase (p < .05). At Day 30, BACKT‐treated eyes showed lower levels of superoxide dismutase (SOD) (p = .006) and higher levels of malondialdehyde (MDA) (p = .02). Differences between treatments were not observed for any parameter at any time point (p > .05). 60 days after treatment, OSAvet® parameters tended to return to values assessed at baseline; however, significant differences remained for MG loss (p < .05).ConclusionsTwice‐daily instillation of KT, containing or not BAC, for 30 consecutive days shortened NI‐TFBT, decreased GCD, and increased the MG loss in healthy dogs. KT should be used with caution when prescribed for long periods, particularly in patients with tear film abnormalities. However, future controlled studies using KT, BAC, and other topical NSAIDs are indicated to further support this finding.

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Milestones in Medical Mycology in India

Gugnani

2021

Progress in Mycology

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Oxidative stress in corneal tissue in experimental keratitis due to Aspergillus flavus: Effect of topical voriconazole therapy

Cited by 3 publications

References 37 publications

Corneal Ulcer in Dogs and Cats: Novel Clinical Application of Regenerative Therapy Using Subconjunctival Injection of Autologous Platelet-Rich Plasma

Corneal Ulcer in Dogs and Cats: Novel Clinical Application of Regenerative Therapy Using Subconjunctival Injection of Autologous Platelet-Rich Plasma

Effects of topical ketorolac tromethamine on tear parameters, meibography, goblet cell density, and conjunctival oxidative stress in healthy dogs

Milestones in Medical Mycology in India

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