Oxidative stress following exposure to silver and gold nanoparticles in mice

Shrivastava, Rupal; Kushwaha, Pramod; Bhutia, Yang Chen; Flora, S.J.S.

doi:10.1177/0748233714562623

Cited by 103 publications

(71 citation statements)

References 51 publications

(63 reference statements)

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“…The various concentrations of PVP-and CT-AgNPs (2.5, 10 and 40 µg/ml) used in our study are also similar to previous studies evaluating nanotoxicity and blood compatibility of erythrocytes incubated with AgNPs [17,23]. Our concentrations are comparable with previous studies using AgNPs on human, mouse and fish erythrocytes and the highest dose of 40 µg/ml, is much lower than the optimal dose of AgNPs (200 µg/ml) that has been reported to induce hemolysis in human erythrocytes [17,18,23,[32][33][34]. In fact, in humans following oral exposure, in vivo serum concentration of AgNPs of up to 10µg/ml has been reported [35,36].…”

Section: Discussionsupporting

confidence: 77%

The in Vitro Effect of Polyvinylpyrrolidone and Citrate Coated Silver Nanoparticles on Erythrocytic Oxidative Damage and Eryptosis

Ferdous

Beegam

Tariq

et al. 2018

Cell Physiol Biochem

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Background/Aims: Silver nanoparticles (AgNPs) are increasingly used as antimicrobial agents and drug carriers in various biomedical fields. AgNPs can encounter erythrocytes either directly following intravenous injection, or indirectly via translocation from the site of administration. However, information regarding the pathophysiological effects and possible mechanism of action of AgNPs on the erythrocytes are still inadequately studied. Thus, the aim of our study was to investigate the mechanism underlying the effect of coating and concentration of AgNPs on mouse erythrocytes in vitro. Methods: We studied the interaction of polyvinylpyrrolidone (PVP) and citrate (CT) coated AgNPs (10 nm) at various concentrations (2.5, 10, 40 µg/ml) with mouse erythrocytes in vitro using various techniques including transmission electron microscopy (TEM), hemolysis, and colorimetric measurement of markers of oxidative stress comprising malondialdehyde (MDA), reduced glutathione (GSH), and catalase (CAT). Intracellular calcium (Ca²⁺) was determined using Fura 2AM fluorescence. Annexin V was quantified using ELISA and the caspase 3 was determined both flurometrically and by western blot technique. Results: Following incubation of the erythrocytes with AgNPs, both PVP- and CT- AgNPs induced significant and dose - dependent increase in hemolysis. TEM revealed that both PVP- and CT- AgNPs were taken up by erythrocytes. The erythrocyte susceptibility to lipid peroxidation measured by MDA was significantly increased in both PVP-and CT- AgNPs. The concentration of GSH and CAT activity were significantly decreased by both types of AgNPs. Additionally, PVP- and CT- AgNPs significantly increased intracellular Ca²⁺ in a dose -dependent manner. Likewise, the concentration of the cellular protein annexin V was significantly and dose - dependently enhanced by both types of AgNPs. Furthermore, PVP- and CT- AgNPs induced significant increase in calpain activity in incubated erythrocytes. Conclusion: We conclude that both PVP- and CT- AgNPs causes hemolysis, and are taken up by erythrocytes. Moreover, we demonstrated that AgNPs induces oxidative stress and eryptosis. These findings provide evidence for the potential pathophysiological effect of PVP-and CT- AgNPs on erythrocyte physiology.

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Section: Discussionsupporting

confidence: 77%

The in Vitro Effect of Polyvinylpyrrolidone and Citrate Coated Silver Nanoparticles on Erythrocytic Oxidative Damage and Eryptosis

Ferdous

Beegam

Tariq

et al. 2018

Cell Physiol Biochem

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“…biosensors that need to be able to pass through human kidneys [1][2][3], and catalysts with increased efficiency [4][5][6]. One attractive NP production technique is plasma processing which has many advantages [7][8][9] such as suppression of unwanted agglomeration by Coulomb repulsion and the possibility of fast growth through ion collection.…”

Section: Introductionmentioning

confidence: 99%

On the work function and the charging of small (r≤ 5 nm) nanoparticles in plasmas

et al. 2017

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On the work function and the charging of small (r <= 5 nm) nanoparticles in plasmas, Physics of Plasmas, 2017. 24 electron field emission (EFE), thermionic electron emission (TIE), and electron impact detachment. Here we report theoretical vales of the work function in this size range. Density functional theory (DFT) is used to calculate the work functions for a set of NP charge numbers, sizes and shapes, using copper for a case study. An analytical approximation is shown to give quite accurate work functions provided that >0.4 nm, i.e., consisting of about > 20 atoms, and provided also that the NPs have relaxed to close to spherical shape.For smaller sizes W deviates from the approximation, and also depends on the charge number. Some consequences of these results for nanoparticle charging are outlined. In particular, a decrease in W for NP radius below about 1 nm has fundamental consequences for their charge in a plasma environment, and thereby on the important processes of NP nucleation, early growth, and agglomeration.

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“…17 Hematological alterations, a common hallmark of toxicity, had been demonstrated in mice that were intravenously given gold nanoparticles (AuNPs). 18 Cytotoxic effect was noted in both SiNP-and AuNP-treated mice by Shrivastava et al, 19 and increased reactive oxygen species resulting in oxidative stress damage was demonstrated to be the reason for the noxious effect. However, a recent study performed by Fraga et al 20 to observe the short-and long-term toxicities after a singledose intravenous AuNPs to rats showed no severe acute or delayed toxicity.…”

mentioning

confidence: 99%

In vivo toxicologic study of larger silica nanoparticles in mice

Chan¹,

Liu²,

Chiau³

et al. 2017

IJN

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Silica nanoparticles (SiNPs) are being studied and used for medical purposes. As nanotechnology grows rapidly, its biosafety and toxicity have frequently raised concerns. However, diverse results have been reported about the safety of SiNPs; several studies reported that smaller particles might exhibit toxic effects to some cell lines, and larger particles of 100 nm were reported to be genotoxic to the cocultured cells. Here, we investigated the in vivo toxicity of SiNPs of 150 nm in various dosages via intravenous administration in mice. The mice were observed for 14 days before blood examination and histopathological assay. All the mice survived and behaved normally after the administration of nanoparticles. No significant weight change was noted. Blood examinations showed no definite systemic dysfunction of organ systems. Histopathological studies of vital organs confirmed no SiNP-related adverse effects. We concluded that 150 nm SiNPs were biocompatible and safe for in vivo use in mice.

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Oxidative stress following exposure to silver and gold nanoparticles in mice

Cited by 103 publications

References 51 publications

The in Vitro Effect of Polyvinylpyrrolidone and Citrate Coated Silver Nanoparticles on Erythrocytic Oxidative Damage and Eryptosis

The in Vitro Effect of Polyvinylpyrrolidone and Citrate Coated Silver Nanoparticles on Erythrocytic Oxidative Damage and Eryptosis

On the work function and the charging of small (r≤ 5 nm) nanoparticles in plasmas

In vivo toxicologic study of larger silica nanoparticles in mice

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