Oxidative stress by Haemonchus contortus in lambs: Influence of treatment with zinc edetate

Pivoto, Felipe Lamberti; Torbitz, Vanessa Dorneles; Aires, Adelina Rodrigues; Rocha, José; Severo, Marcelo Machado; Grando, Thirssa H.; Peiter, Mateus; Moresco, Rafael Noal; Rocha, João Batista Teixeira da; Leal, Marta Lizandra do Rêgo

doi:10.1016/j.rvsc.2015.07.001

Cited by 12 publications

(12 citation statements)

References 14 publications

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“…However, there are few studies on dictyocaulosis regarding its effect on reactive species. On the other hand, the relation between ROS and oxidative damage on DNA was evaluated by Gao et al (2015), and the consequent negative effect on the host was already described in studies with other nematodes, such as Haemonchus contortus (Pivoto et al, 2015). In this study, levels of ROS, lipid peroxidation and the anti-oxidant status were measured in cows naturally infected by D. viviparus , and the oxidative profile in sera of these animals showed an increase in variables of the oxidative damage.…”

Section: Discussionmentioning

confidence: 95%

Oxidative stress in dairy cows naturally infected with the lungwormDictyocaulus viviparus(Nematoda: Trichostrongyloidea)

Silva

Baldissera

et al. 2016

J. Helminthol.

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The aim of this study was to analyse the oxidative and anti-oxidant status in serum samples from dairy cows naturally infected by Dictyocaulus viviparus and its relation with pathological analyses. The diagnosis of the disease was confirmed by necropsy of one dairy cow with heavy infection by the parasite in the lungs and bronchi. Later, blood and faeces were collected from another 22 cows from the same farm to measure reactive oxygen species (ROS) levels, thiobarbituric acid-reactive substances (TBARS), catalase (CAT) and superoxide dismutase (SOD) activities on day 0 (pre-treatment) and day 10 (post-treatment with eprinomectin). Faecal examination confirmed the infection in all lactating cows. However, the number of D. viviparus larvae per gram of faeces varied between animals. Cows showed different degrees of severity according to respiratory clinical signs of the disease (cough and nasal secretion). Further, they were classified and divided into two groups: those with mild (n = 10) and severe disease (n = 12). Increased levels of TBARS (P < 0.001), ROS (P = 0.002) and SOD activity (P < 0.001), as well as reduced CAT activity (P < 0.001) were observed in cows with severe clinical signs of the disease compared to those with mild clinical signs. Eprinomectin treatment (day 10) caused a reduction of ROS levels (P = 0.006) and SOD activity (P < 0.001), and an increase of CAT activity (P = 0.05) compared to day 0 (pre-treatment). TBARS levels did not differ with treatment (P = 0.11). In summary, increased ROS production and lipid peroxidation altered CAT and SOD activities, as an adaptive response against D. viviparus infection, contributing to the occurrence of oxidative stress and severity of the disease. Treatment with eprinomectin eliminated the infection, and thus minimized oxidative stress in dairy cows.

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Section: Discussionmentioning

confidence: 95%

Oxidative stress in dairy cows naturally infected with the lungwormDictyocaulus viviparus(Nematoda: Trichostrongyloidea)

Silva

Baldissera

et al. 2016

J. Helminthol.

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“…The significant depression of TAC level observed in sera of infected animals (G1, G2, and G3) may be attributed to the oxidative stress developed by H. contortus. Pivoto et al [58] determined the total oxidation status and antioxidant capacity of lambs infected with H. contortus and reported increased oxidative stress in lambs' sera. On the other hand, increase in levels of Ferric Reducing Ability of Plasma (FRAP), an indicator of TAC in sera of lambs, was observed during infection with H. contortus [59].…”

Section: Discussionmentioning

confidence: 99%

Therapeutic Effect of Balanites Aegyptiaca Fruit’s Ethanol Extract in Egyptian Baladi Goats Experimentally Infected with Haemonchus Contortus: Blood Serum Biochemical, Oxidative Stress Markers and Pathological Studies

et al. 2020

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the native breed of goats that is located in the Nile Delta and along the Nile Valley is known as Baladi goats. Amongst other breeds of goats, the Baladi goats have a great economic value [1] and play an essential role in the economy of small, marginal farmers and landless labourers in Egypt [2]. T HE THERAPEUTIC effect of Balanites aegyptiaca fruit's ethanolic extract (BAF-EE) in goats experimentally infected with Haemonchus contortus (H. contortus) was evaluated. Serum biochemical parameters, tissue oxidative stress status and pathological changes were investigated. Twelve goats were divided into 4 equal groups, G1 (infected-untreated), G2 (infected-BAF-EE treated), G3 (infected-albendazole treated) and G4 (uninfected-BAF-EE treated). Infection was conducted at 0-time, whereas treatment with BAF-EE and albendazole was given in the 5 th week post infection (PI). Blood samples were drawn on 0-time, 3 rd week PI and then biweekly till the 9 th week for serum biochemical analyses. At the end of experiment, all animals were slaughtered to estimate oxidative stress markers and histopathological alterations in the abomasal and hepatic tissues. Serum biochemistry of infected animals showed significant depression of total serum proteins, albumin, albumin/ globulin ratio, total antioxidant capacity and calcium levels. The activity of AST, ALT and γGT and the cholesterol, urea and creatinine levels were significantly elevated in the serum of infected animals. Glutathione peroxidase values decreased significantly in tissue homogenate of G1 and G3 goats than those in G2 and G4 goats. Malondialdehyde followed a reverse course. Abomasa of infected animals in G1 revealed inflammation, necrosis, petechial hemorrhages and cellular infiltration. Liver showed bile duct hyperplasia, degeneration of hepatocytes and cell infiltration. Intensity of alterations were lower in infected-treated animals. In conclusion, BAF-EE has hepatoprotective effect and can improve serum biochemical alterations and oxidative stress status in goats infected with H. contortus, justifying its use as a safe natural anthelmintic agent.

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“…The activity of blood GPx was higher, and lipid peroxidation in the serum was lower, at the end of the experiment in the groups treated with zinc than in the untreated group. Pivoto et al [ 60 ] also demonstrated that zinc could help reduce the oxidative stress caused by H. contortus in lambs. Our findings indicate that treatment with organic zinc alone or in combination with herbal nutraceuticals could potentially provide natural antioxidants for minimizing oxidative stress in nematode infected lambs.…”

Section: Discussionmentioning

confidence: 99%

Effects of herbal nutraceuticals and/or zinc against Haemonchus contortus in lambs experimentally infected

et al. 2018

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BackgroundThe gastrointestinal parasitic nematode Haemonchus contortus is a pathogenic organism resistant to several anthelmintics. This study assessed the efficacy of a medicinal herbal mixture (Herbmix) and organic zinc, as an essential trace element for the proper functioning of both unspecific and specific immune defensive mechanisms, against experimental infections with H. contortus in lambs. All lambs were infected orally with approximately 5000 third-stage larvae of a strain of H. contortus susceptible to anthelmintics (MHco1). Twenty-four female lambs 3–4 months of age were divided into four groups: unsupplemented animals (control), animals supplemented with Herbmix (Hmix), animals supplemented with organic zinc (Zn) and animals supplemented with Herbmix and organic zinc (Hmix+Zn). Eggs per gram (EPG) of faeces were quantified 20, 28, 35, 42, 49, 56, 62 and 70 d post-infection and mean abomasal worm counts were assessed 70 d post-infection. Samples of blood were collected from each animal 7, 35, 49 and 70 d post-infection.ResultsQuantitative analyses of the bioactive compounds in Herbmix identified three main groups: flavonoids (9964.7 μg/g), diterpenes (4886.1 μg/g) and phenolic acids (3549.2 μg/g). Egg counts in the lambs treated with Hmix, Zn and Hmix+Zn decreased after 49 d. The EPGs in the Zn and Hmix+Zn groups were significantly lower on day 56 (P < 0.05 and P < 0.01, respectively), and the EPGs and mean worm counts were significantly lower on day 70 in all supplemented groups (P < 0.05 and P < 0.01). Hemograms of complete red blood cells of each animal identified clinical signs of haemonchosis after day 35. Serum calprotectin concentrations and IgA levels were significantly affected by treatment. The treatment influenced serum malondialdehyde concentrations (P < 0.05) and sulfhydryl groups (P < 0.01) of antioxidant status. The mineral status was unaltered in all lambs.ConclusionA direct anthelmintic impact on the viability of nematodes was not fully demonstrated, but the treatments with herbal nutraceuticals and zinc likely indirectly contributed to the increase in the resistance of the lambs to nematode infection.

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Oxidative stress by Haemonchus contortus in lambs: Influence of treatment with zinc edetate

Cited by 12 publications

References 14 publications

Oxidative stress in dairy cows naturally infected with the lungwormDictyocaulus viviparus(Nematoda: Trichostrongyloidea)

Oxidative stress in dairy cows naturally infected with the lungwormDictyocaulus viviparus(Nematoda: Trichostrongyloidea)

Therapeutic Effect of Balanites Aegyptiaca Fruit’s Ethanol Extract in Egyptian Baladi Goats Experimentally Infected with Haemonchus Contortus: Blood Serum Biochemical, Oxidative Stress Markers and Pathological Studies

Effects of herbal nutraceuticals and/or zinc against Haemonchus contortus in lambs experimentally infected

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