Oxidative stress in dairy cows naturally infected with the lungwormDictyocaulus viviparus(Nematoda: Trichostrongyloidea)

Abstract

The aim of this study was to analyse the oxidative and anti-oxidant status in serum samples from dairy cows naturally infected by Dictyocaulus viviparus and its relation with pathological analyses. The diagnosis of the disease was confirmed by necropsy of one dairy cow with heavy infection by the parasite in the lungs and bronchi. Later, blood and faeces were collected from another 22 cows from the same farm to measure reactive oxygen species (ROS) levels, thiobarbituric acid-reactive substances (TBARS), catalase (CAT) and superoxide dismutase (SOD) activities on day 0 (pre-treatment) and day 10 (post-treatment with eprinomectin). Faecal examination confirmed the infection in all lactating cows. However, the number of D. viviparus larvae per gram of faeces varied between animals. Cows showed different degrees of severity according to respiratory clinical signs of the disease (cough and nasal secretion). Further, they were classified and divided into two groups: those with mild (n = 10) and severe disease (n = 12). Increased levels of TBARS (P < 0.001), ROS (P = 0.002) and SOD activity (P < 0.001), as well as reduced CAT activity (P < 0.001) were observed in cows with severe clinical signs of the disease compared to those with mild clinical signs. Eprinomectin treatment (day 10) caused a reduction of ROS levels (P = 0.006) and SOD activity (P < 0.001), and an increase of CAT activity (P = 0.05) compared to day 0 (pre-treatment). TBARS levels did not differ with treatment (P = 0.11). In summary, increased ROS production and lipid peroxidation altered CAT and SOD activities, as an adaptive response against D. viviparus infection, contributing to the occurrence of oxidative stress and severity of the disease. Treatment with eprinomectin eliminated the infection, and thus minimized oxidative stress in dairy cows.

Redox Biomarker Baseline Levels in Cattle Tissues and Their Relationships with Meat Quality

“…In line with the present results, it was recorded that the lipid oxidation biomarkers were significantly lower in the LIM breed compared to SIM and three other cattle breeds in China [51]. Enhanced lipid peroxidation levels have also been reported in infected [55][56][57], ketotic [58] or periparturient [59,60] dairy cows, indicating the significant one-dimensional effect that environmental and physiological conditions have on redox parameters, and the necessity of their documentation before any nutritional intervention. Therefore, the overall reduced endogenous antioxidant capacity of the CHA and SIM breeds compared to LIM might explain a possible failure in case a similar antioxidant treatment is implemented.…”

Μελέτη Δεικτών Οξειδοαναγωγικής Κατάστασης Στο Κρέας Παραγωγικών Ζώων

“…Σε προηγούμενη μελέτη έχει τονιστεί ο συσχετισμός της δυσλειτουργίας αυτού του μυός με την στη φυλή LIM σε σύγκριση με τη SIM και τρεις άλλες φυλές βοοειδών στην Κίνα(Xie et al, 2012). Έχουν επίσης αναφερθεί αυξημένα επίπεδα υπεροξείδωσης λιπιδίων σε ζώα με λοιμώδη νοσήματα (Α.F Fidan et al, 2010;da Silva et al, 2017;. Glοmbοwsky et al, 2017), με κέτωση(Li et al, 2016) ή σε έγκυα(Hanschke et al, 2016;Kuhn et al, 2018) βοοειδή, υποδεικνύοντας τη σημαντική .…”