Oxidative stress and protection against reactive oxygen species in the pre-implantation embryo and its surroundings

Guérin, Philippe J.

doi:10.1093/humupd/7.2.175

Cited by 1,159 publications

(999 citation statements)

References 120 publications

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“…Although we do not have evidence, it might be possible that the surrounding microenvironment could be different when fewer embryos are cultured than in large groups. Exogenous reactive oxygen species are produced by many sources as high oxygen tension, exposure to visible light or excess of glucose in the medium among others (reviewed by Gué rin et al, 2001) and it is known that oxidative stress induces DNA fragmentation in embryos (Kitagawa et al, 2004). In any case, the present work showed that supplementation of EGF plus IGF-I or EGF alone when embryos were cultured in small groups diminished apoptosis rate at similar levels to those of larger groups.…”

Section: Discussionsupporting

confidence: 57%

Effect of supplementation of different growth factors in embryo culture medium with a small number of bovine embryos on in vitro embryo development and quality

Ahumada

Salvador

Cebrián-Serrano

et al. 2013

Animal

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When embryos are cultured individually or in small groups, blastocyst yield efficiency and quality are usually reduced. The aim of this work was to investigate the effect of supplementation of the embryo culture medium (CM) with several growth factors (GFs) on embryo development and apoptosis rate when a reduced number of embryos were in vitro cultured. Two experimental studies (ES) were carried out. In ES 1, five treatments were tested to study the effect of GF on embryo development: Control (,30 to 50 embryos cultured in 500 ml of CM); Control 5 (Five embryos cultured in 50 ml microdrops of CM), without addition of GF in either of the two control groups; epidermal GF (EGF); IGF-I; and transforming GF-a (TGF-a) (Five embryos were cultured in 50 ml microdrops of CM with 10 ng/ml EGF, 10 ng/ml IGF-I or 10 ng/ml TGF-a, respectively). In ES 2, following the results obtained in ES 1, four different treatments were tested to study their effect on embryo development and quality (number of cells per blastocyst and apoptotic rate): Control; Control 5; EGF, all three similar to ES 1; EGF 1 IGF-I group (five embryos cultured in 50 ml microdrops of CM with 10 ng/ml EGF and 10 ng/ml IGF-I). In both ESs, it was observed that a higher proportion of embryos cultured in larger groups achieved blastocyst stage than embryos cultured in reduced groups (22.6% v. 14.0%, 12.6% and 5.3% for Control v. Control 5, IGF-I, TGF-a groups in ES 1, and 24.9% v. 17.1% and 19.0% for Control v. Control 5 and EGF in ES 2, respectively; P , 0.05), with the exception of embryos cultured in medium supplemented with EGF (18.5%) or with EGF 1 IGF-I (23.5%), in ES 1 and ES 2, respectively. With regard to blastocyst quality, embryos cultured in reduced groups and supplemented with EGF, alone or combined with IGF-I, presented lower apoptosis rates than embryos cultured in reduced groups without GF supplementation (11.6% and 10.5% v. 21.9% for EGF, EGF 1 IGF-I and Control 5 groups, respectively; P , 0.05). The experimental group did not affect the total number of cells per blastocyst. In conclusion, this study showed that supplementation of the CM with EGF and IGF could partially avoid the deleterious effect of in vitro culture of small groups of bovine embryos, increasing blastocyst rates and decreasing apoptosis rates of these blastocysts.Keywords: bovine embryo, reduced groups, in vitro culture, growth factor, apoptosis ImplicationsIn bovine, in vitro embryo production (IVEP) is especially interesting when applying the ovum pick-up technique (OPU). However, a small number of oocytes per female are usually recovered after OPU, and when embryos are cultured individually or in a reduced group blastocyst yield efficiency and quality are generally reduced. The improvement of IVEP efficiency and quality with low numbers of embryos would be of great interest for applying this technique in the dairy industry. IntroductionIn vitro embryo production (IVEP) has numerous applications in various scientific areas such as animal production or biomedicine. IVEP-rela...

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Section: Discussionsupporting

confidence: 57%

Effect of supplementation of different growth factors in embryo culture medium with a small number of bovine embryos on in vitro embryo development and quality

Ahumada

Salvador

Cebrián-Serrano

et al. 2013

Animal

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“…The inhibition of CPT1 leads to the generation of reactive oxygen species 22. Oxidative stress in the endometrium has been associated with failures of embryo implantation and embryo development 37. The fact that these anti‐oxidative stress genes were down‐regulated in the thin endometrium suggests that a decreased response to oxidative stress is associated with implantation failure.…”

Section: Discussionmentioning

confidence: 99%

Thin endometrium transcriptome analysis reveals a potential mechanism of implantation failure

Maekawa

Tanaka

Mihara

et al. 2017

Reprod Medicine & Biology

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AimAlthough a thin endometrium has been well recognized as a critical factor in implantation failure, little information is available regarding the molecular mechanisms. The present study investigated these mechanisms by using genome‐wide mRNA expression analysis.MethodsThin and normal endometrial tissue was obtained from a total of six women during the mid‐luteal phase of the menstrual cycle. The transcriptomes were analyzed with a microarray. Differentially expressed genes were classified according to Gene Ontology (GO) terms and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways.ResultsThe study identified 318 up‐regulated genes and 322 down‐regulated genes in the thin endometrium, compared to the control endometrium. The GO and KEGG pathway analyses indicated that the thin endometrium possessed aberrantly activated immunity and natural killer cell cytotoxicity that was accompanied by an increased number of inflammatory cytokines, such as IFN‐γ. Various genes that were related to metabolism and anti‐oxidative stress were down‐regulated in the thin endometrium.ConclusionImplantation failure in the thin endometrium appears to be associated with an aberrantly activated inflammatory environment and aberrantly decreased response to oxidative stress.

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“…Although oxygen tension in the oviduct and uterus of most mammalian species has been found to range from 2 % to 8 % [8,28], many laboratories are still using atmospheric oxygen concentrations (20 %) for the culture of human embryos. Elevated and non-physiological O 2 concentrations could create unfavorable conditions in the substrate to produce free oxygen radicals that cause oxidative stress, which may be involved in the etiology of defective embryo development with higher rates of fragmentation [2,13]. Reactive oxygen species (ROS) may originate from embryo metabolism and/or embryo surroundings [12].…”

Section: Introductionmentioning

confidence: 99%

The effect of two distinct levels of oxygen concentration on embryo development in a sibling oocyte study

Kasterstein

Strassburger

Komarovsky

et al. 2013

J Assist Reprod Genet

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Purpose This prospective randomized study used sibling oocytes of 258 women with ≥8 oocytes to compare the effect of 5 % O 2 versus 20 % O 2 concentrations on embryo development and clinical outcome. Methods Oocytes of each case were divided between incubators with either 5 % or 20 % O 2 concentration. Outcome measures were fertilization, cleavage, embryo quality, blastocyst formation, and implantation, pregnancy and live birth rates. Results Fertilization and cleavage rates were similar in both groups. The 5 % O 2 group had significantly more blastomeres (P<0.05) and more top-quality embryos on day 3 (P<0.02), as well as significantly more available embryos for transfer (31.6 % vs. 23.1 % for the 20 % O 2 group; P<0.0001). There were significantly more cycles with good embryos in the 5 % group (76/258) than in the 20 % group (38/258) (P<0.0001). Implantation and pregnancy rates were significantly higher for 5 % O 2 embryos (P<0.03 and P<0.05, respectively). Live birth rates per embryo transfer were 34.2 % and 15.8 %, respectively, P<0.05. Conclusions Implantation, pregnancy and live birth rates are higher, and more good quality embryos are available for transfer and freezing with reduced rather than with atmospheric oxygen concentrations during embryo incubation.

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Oxidative stress and protection against reactive oxygen species in the pre-implantation embryo and its surroundings

Cited by 1,159 publications

References 120 publications

Effect of supplementation of different growth factors in embryo culture medium with a small number of bovine embryos on in vitro embryo development and quality

Effect of supplementation of different growth factors in embryo culture medium with a small number of bovine embryos on in vitro embryo development and quality

Thin endometrium transcriptome analysis reveals a potential mechanism of implantation failure

The effect of two distinct levels of oxygen concentration on embryo development in a sibling oocyte study

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