Thin endometrium transcriptome analysis reveals a potential mechanism of implantation failure

Maekawa, Ryo; Tanaka, Toshiaki; Mihara, Yumiko; Sato, Shun; Okada, Maki; Jozaki, Kosuke; Kajimura, Takuya; Asada, Hiromi; Tamura, Hiroshi; Takasaki, Akihisa

doi:10.1002/rmb2.12030

Cited by 46 publications

(39 citation statements)

References 35 publications

(93 reference statements)

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“…Maekawa et al [ 27 ] found an aberrant Th1-pro-inflammatory/Th2-anti-inflammatory balance and increased cytotoxic condition in patients with thin endometrium. Genome-wide mRNA expression analysis was used in order to show the different expression profiles that exist in case of patients with thin endometrium compared to the control group with an endometrium ≥7 mm [ 27 ].…”

Section: Discussionmentioning

confidence: 99%

Management of a Thin Endometrium by Hysteroscopic Instillation of Platelet-Rich Plasma Into The Endomyometrial Junction: A Pilot Study

Agarwal

Mettler

Jain

et al. 2020

JCM

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In patients whose embryo transfer has been previously canceled due to a thin endometrium, the injection of platelet-rich plasma (PRP) guided by hysteroscopy into the endomyometrial junction improves endometrial thickness and vascularity. This may well serve as a novel approach for the management of these patients. In this study, 32 patients aged between 27 and 39 years, suffering from primary or secondary infertility, were selected for hysteroscopic instillation of PRP. This cross-sectional study included a retrospective assessment of the improvement of endometrial thickness (>7 mm) on the commencement of progesterone treatment in 24 of 32 patients (75%) after hysteroscopy-guided injections of PRP into the subendometrial zone. After PRP instillation, the endometrium was 7 mm or thicker in 24 of 32 patients, and all 24 patients underwent frozen embryo transfer. Moreover, 12 of 24 patients who underwent embryo transfer conceived, whereas 10 had a clinical pregnancy with visualization of cardiac activity at 6 weeks and two had a biochemical pregnancy. Our approach of PRP injection into the subendometrial region is consistent with the histologically proven regeneration of the endometrium from the endomyometrial junction. We observed an improvement of endometrial thickness and higher pregnancy rates in cases of previously canceled embryo transfer due to a thin endometrium.

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Section: Discussionmentioning

confidence: 99%

Management of a Thin Endometrium by Hysteroscopic Instillation of Platelet-Rich Plasma Into The Endomyometrial Junction: A Pilot Study

Agarwal

Mettler

Jain

et al. 2020

JCM

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“…Although no significant enrichment was observed, it was noteworthy that four out of 18 DEGs were related to “cytoskeleton organization” ( MNS1, TTLL1 , TPPP , UGT8 ). Interestingly, UGT8 was down-regulated in the endometrium of women affected by implantation failure [ 54 ]. The gene FCER1A is associated with “positive regulation of granulocyte macrophage colony-stimulating factor production” was less expressed in NB-pregnant heifers.…”

Section: Discussionmentioning

confidence: 99%

Transcriptome profiles in peripheral white blood cells at the time of artificial insemination discriminate beef heifers with different fertility potential

et al. 2018

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BackgroundInfertility is a longstanding limitation in livestock production with important economic impact for the cattle industry. Female reproductive traits are polygenic and lowly heritable in nature, thus selection for fertility is challenging. Beef cattle operations leverage estrous synchronization in combination with artificial insemination (AI) to breed heifers and benefit from an early and uniform calving season. A couple of weeks following AI, heifers are exposed to bulls for an opportunity to become pregnant by natural breeding (NB), but they may also not become pregnant during this time period. Focusing on beef heifers, in their first breeding season, we hypothesized that: a- at the time of AI, the transcriptome of peripheral white blood cells (PWBC) differs between heifers that become pregnant to AI and heifers that become pregnant late in the breeding season by NB or do not become pregnant during the breeding season; and b- the ratio of transcript abundance between genes in PWBC classifies heifers according to pregnancy by AI, NB, or failure to become pregnant.ResultsWe generated RNA-sequencing data from 23 heifers from two locations (A: six AI-pregnant and five NB-pregnant; and B: six AI-pregnant and six non-pregnant). After filtering out lowly expressed genes, we quantified transcript abundance for 12,538 genes. The comparison of gene expression levels between AI-pregnant and NB-pregnant heifers yielded 18 differentially expressed genes (DEGs) (ADAM20, ALDH5A1, ANG, BOLA-DQB, DMBT1, FCER1A, GSTM3, KIR3DL1, LOC107131247, LOC618633, LYZ, MNS1, P2RY12, PPP1R1B, SIGLEC14, TPPP, TTLL1, UGT8, eFDR≤0.02). The comparison of gene expression levels between AI-pregnant and non-pregnant heifers yielded six DEGs (ALAS2, CNKSR3, LOC522763, SAXO2, TAC3, TFF2, eFDR≤0.05). We calculated the ratio of expression levels between all gene pairs and assessed their potential to classify samples according to experimental groups. Considering all samples, relative expression from two gene pairs correctly classified 10 out of 12 AI-pregnant heifers (P = 0.0028) separately from the other 11 heifers (NB-pregnant, or non-pregnant).ConclusionThe transcriptome profile in PWBC, at the time of AI, is associated with the fertility potential of beef heifers. Transcript levels of specific genes may be further explored as potential classifiers, and thus selection tools, of heifer fertility.Electronic supplementary materialThe online version of this article (10.1186/s12864-018-4505-4) contains supplementary material, which is available to authorized users.

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“…Comparing our results with the atlas of the RNA editome in Sus scrofa skeletal muscle [78], we confirmed 22 of 57 RNA editing sites expressed in the endometrial tissues. Significant AAF changes in 3 UTR TMEM161A and upstream from GFM1 could be linked with the potential mechanism of implantation failure [79] and intrauterine growth retardation [80], respectively. SNV-calling methods are valuable tools which expand our understanding of post-transcriptional modifications in the porcine genome.…”

Section: Discussionmentioning

confidence: 99%

Transcriptome, Spliceosome and Editome Expression Patterns of the Porcine Endometrium in Response to a Single Subclinical Dose of Salmonella Enteritidis Lipopolysaccharide

Paukszto

Mikołajczyk

Jastrzebski

et al. 2020

IJMS

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Endometrial infections at a young age can lead to fertility issues in adulthood. Bacterial endotoxins, such as lipopolysaccharide (LPS), can participate in long-term molecular changes even at low concentrations. Lipopolysaccharide plays a crucial role in the progression of septic shock, inflammation and auto-immune diseases. The aim of this study was to describe transcriptomic modulations in the porcine endometrium, induced in vivo by a single subclinical dose of LPS from Salmonella Enteritidis. which did not produce clinical symptoms of toxicity. The RNA-seq methodology was applied to reveal 456 differentially expressed regions, including 375 genes, four long noncoding RNAs, and 77 other unclassified transcripts. Two independent methods confirmed 118 alternatively spliced genes that participate i.a., in the formation of the MHC-I complex and the adaptive immune response. Single nucleotide variant-calling algorithms supported the identification of 3730 allele-specific expression variants and 57 canonical A-to-I RNA editing sites. The results demonstrated that the differential expression of genes involved in inflammation, immune response, angiogenesis and endometrial development may be maintained for up to 7 days after exposure to LPS. RNA editing sites and long noncoding RNAs (lncRNAs) play an important role in transcriptional regulatory machinery in the porcine endometrium in response to LPS administration.

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Thin endometrium transcriptome analysis reveals a potential mechanism of implantation failure

Cited by 46 publications

References 35 publications

Management of a Thin Endometrium by Hysteroscopic Instillation of Platelet-Rich Plasma Into The Endomyometrial Junction: A Pilot Study

Management of a Thin Endometrium by Hysteroscopic Instillation of Platelet-Rich Plasma Into The Endomyometrial Junction: A Pilot Study

Transcriptome profiles in peripheral white blood cells at the time of artificial insemination discriminate beef heifers with different fertility potential

Transcriptome, Spliceosome and Editome Expression Patterns of the Porcine Endometrium in Response to a Single Subclinical Dose of Salmonella Enteritidis Lipopolysaccharide

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