Oxidative stress and prolidase activity in women with uterine fibroids

Abstract

The aim of this study was to investigate oxidative stress markers and prolidase activity in serum and tissue samples of women with uterine fibroids, with further analysis on position and size. Lipid hydroperoxide, ceruloplasmin, catalase, arylesterase, free sulfhydryl group activity and prolidase activity levels were measured in fibroid tissue, myometrial tissue and serum of the same patients (n = 51), at the same time. Results show that ceruloplasmin, catalase, arylesterase, free sulfhydryl group and prolidase activities were higher in fibroid tissue than those in myometrial tissue (p = 0.003, 0.009, 0.004, 0.02, 0.008, respectively). Serum levels of catalase and prolidase were lower, and arylesterase and free sulfhydryl groups were higher in the fibroid group than those in the control group (p < 0.001 for all). Fibroid volume in submucosal subgroup of the fibroid group yield significant correlation with ceruloplasmin, catalase, arylesterase and prolidase activities (r = 0.84, p = 0.02; r = 0.93, p < 0.001; r = 0.63, p = 0.049 and r = 0.87, p = 0.01, respectively). Despite the lack of statistical significance, the highest levels of prolidase activity were found in fibroid samples, especially in submucosal ones. It is concluded that this study demonstrated increased antioxidative repair system in the fibroid tissue compared to the myometrium and serum of the same patients. Additionally, higher pathophysiological potential of the submucosal fibroids over intramural and subserosal fibroids were shown with the levels of oxidative stress markers and prolidase activity levels.

Increased Serum Oxidative Stress Markers in Women with Uterine Leiomyoma

“…Estrogen-induced ROS play important roles in cell transformation, cell proliferation, migration and invasion by increasing genomic instability and influencing redox sensitive transcription factors [21]. Although several reports have suggested the role of the oxidative stress in the development of uterine leiomyomas [22–25], no study has investigated the markers of oxidative stress in the sera of patients. Such study could be useful to establish a relationship between the development of the tumor and to determine the pathways involved.…”

Nicotinamide Adenine Dinucleotide Phosphate Oxidase Is Differentially Regulated in Normal Myometrium Versus Leiomyoma

“…[5][6][7][8] This study gathered further evidence to support a role of oxidative stress in the pathophysiology of fibroids. We have demonstrated the overexpression of NOX in fibroid as compared to normal myometrial tissues and cells.…”

Assessment of Tumor markers, C-reactive Protein, Cortisol and Total Plasma Peroxides Levels in Uterine Leiomyoma Patients

“…But the free radicals are regulated by superoxide dismutase also released into the uterus (Vural et al, 2012). Since Bianchi et al (2001) and Mandavilli et al (2002) reported that higher level of hydrogen peroxide was generated from cells that have mitochondrial DNA mutation during tumor development and progression, the deregulated level of TPP observed in our leiomyoma patients could be due to enhanced free radical generation in the macrophage infiltrated myoma tissues.…”

“…In a normal uterus, there is expression of superoxide dismutase that regulates the constantly generated reactive oxygen species, which serve as a local protector of the uterus against infectious agents (Vural et al, 2012). But in a leiomyoma tissue, activation of infiltrating macrophages generate more free radicals, secrete interleukin-6 and several growth hormones including epidermal growth factor, transforming growth factor-α, transforming growth factor-β, heparin-binding epidermal growth factor, acidic fibroblast growth factor, basic fibroblast growth factor, vascular endothelial growth factor, insulin-like growth factor, platelet-derived growth factor, activin and myostatin in myometrium and in leiomyomas (Ciarmela et al, 2011).…”