Oxidative stress and motility in tench Tinca tinca spermatozoa

Dzyuba, Viktoriya; Cosson, Jacky; Dzyuba, Borys; Rodina, Marek

doi:10.17221/8238-cjas

Cited by 10 publications

(7 citation statements)

References 25 publications

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“…The oxidative stress can be evaluated by several methods. Results of these studies show that several protections against the oxidative stress are present in fish sperm cells and in the seminal fluid [31,120].…”

Section: Respiration Oxidative Stress Free Radicals and Dna Damagementioning

confidence: 99%

Fish Sperm Physiology: Structure, Factors Regulating Motility, and Motility Evaluation

Cosson¹

2019

Biological Research in Aquatic Science

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For reproduction, most fish species adopt external fertilization: their spermatozoa are delivered in the external milieu (marine-or freshwater) that represents both a drastic environment and a source of signals that control the motility function. This chapter is an updated overview of the signaling pathways going from external signals such as osmolarity and ionic concentration and their membrane reception to their transduction through the membrane and their final reception at the flagellar axoneme level. Additional factors such as energy management will be addressed as they constitute a limiting factor of the motility period of fish spermatozoa. Modern technologies used nowadays for quantitative description of fish sperm flagella in movement will be briefly described as they are more and more needed for prediction of the quality of sperm used for artificial propagation of many fish species used in aquaculture. The chapter will present some applications of these technologies and the information to which they allow access in some aquaculture species.Biological Research in Aquatic Science 2 the current knowledge with respect to (1) membrane reception of the activation signal and its transduction through the spermatozoon plasma membrane via the external membrane components such as ion channels or aquaporins; (2) cytoplasmic trafficking of the activation signal; (3) final steps of the signaling, including signal transduction to the axonemal machinery, and activation of axonemal dynein motors and regulation of their activity; (4) signaling involved in guidance processes that control the sperm/egg approach and meeting; and (5) pathways supplying energy for the short flagellar motility period of fish spermatozoa. For each step in this signaling process, quantitative methods were developed to evaluate the quality of the sperm samples that are used for aquaculture propagation of many fish species. These methods as well as examples of their usefulness for application to fish artificial reproduction are presented in the last part of this chapter. Fish sperm structure and spermatogenesisThe main traits of the flagellar mechanics of spermatozoa and the main factors that regulate their motility have been described in details in a recent review chapter by Cosson et al. [1]. Structure of fish spermatozoa: a brief presentationCompared to mammalian spermatozoa, the structure of a fish spermatozoon is qualified as "simple sperm", mostly because the flagellum structure does not include additional columns flanking the motor part (axoneme) that are present in mammal sperm. In teleost fish, spermatozoa generally have no acrosome (in contrast to chondrostean such as sturgeon), and the impenetrable chorion presents a micropyle that gives access to the membrane of the oocyte. Spermatozoa often show a spherical nucleus with homogenous, highly condensed chromatin, a nuclear fossa, a midpiece of variable size with or without a cytoplasmic channel, and one or two long flagella [2]. Moreover, fish spermatozoa can be classified into two forms, a...

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Section: Respiration Oxidative Stress Free Radicals and Dna Damagementioning

confidence: 99%

Fish Sperm Physiology: Structure, Factors Regulating Motility, and Motility Evaluation

Cosson¹

2019

Biological Research in Aquatic Science

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“…Therefore, it is necessary to ensure optimal genetic sires selection (Zhang et al 2015) as well as semen processing including semen collection (Dzyuba et al 2015), dilution (Meamar et al 2016), slow cooling to 4-6°C (Kristan et al 2014), and equilibration (Beran et al 2013b) as well as cryopreservation maintaining spermatozoa fertilization capability (Andrabi 2007) evaluated objectively if possible (Simonik et al 2015).…”

Section: Introductionmentioning

confidence: 99%

Equilibration and freezing interactions affecting bull sperm characteristics after thawing

Doležalová¹,

Stádník²,

Biniová³

et al. 2016

CZECH J ANIM SCI

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ABSTRACT:The objective was to determine effects of equilibration length and freezing curve type as well as their interactions on motility and live spermatozoa proportion in bull sperm after thawing. The ejaculates of 6 sires were repeatedly collected. Fresh semen was diluted with one extender and divided into 3 groups equilibrated for 30, 120, and 240 min. Subsequently, half straws of each group were frozen using standard 3-phase or 2-phase freezing curve differing in the rate of temperature decrease. Th e spermatozoa motility (M) was evaluated immediately after thawing and at 30, 60, 90, and 120 min of thermodynamic test (TDT). Live spermatozoa proportion was evaluated after thawing and at the end of TDT. Average of spermatozoa motility (AM), decrease of spermatozoa motility (MD), average proportion of live spermatozoa (ALS), and decrease of live spermatozoa proportion (DLS) through the TDT were calculated. Significant inter-sire differences in AM (0.45-17.0%; P < 0.05-0.01), MD (0.76-12.57%; P < 0.05-0.01), and ALS (0.99-23.8%; P < 0.01) were detected. The longest equilibration ensured the highest M during TDT and AM (+2.72 and +4.58%; P < 0.05-0.01), however higher MD (+4.06%; P < 0.01) compared to standard length as well. Straws freezed using 2-phase curve achieved higher M through TDT, AM (+7.3%; P < 0.01) as well as ALS (+11.77%; P < 0.01). The 2-phase curve presented higher M compared to the 3-phase freezing curve within all equilibration lengths. Significant differences in AM, MD,; P < 0.05-0.01) between equilibration length vs freezing curve interactions were determined. Results document the importance of equilibration length, freezing curve, and their interaction effect on live spermatozoa proportion and sperm motility after thawing as well as necessity of individual conditions for bulls semen processing and insemination doses production.

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“…Sperm motility is widely used as a basic marker of semen or spermatozoa quality (Dzyuba et al 2015;Zhang et al 2015;Petelak and Krylov 2016;Simonik et al 2016). Previous studies focusing on the effects of the above-mentioned substances differ in their conclusions as well as in their methodological approaches.…”

Section: Resultsmentioning

confidence: 99%

Effects of zearalenone, α-zearalenol, and genistein on boar sperm motility in vitro

Krejcárková¹,

Folková²,

Šimoník³

et al. 2017

CZECH J ANIM SCI

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Krejcárková A., Folková P., Šimoník O., Šašková M., Krejčířová R., Drábek O., Rajmon R. (2017): Effects of zearalenone, α-zearalenol, and genistein on boar sperm motility in vitro. Czech J. Anim. Sci., 62, 435-445.Genistein (GEN) and zearalenone (ZEA), environmental oestrogens commonly present in feedstuff for pigs, are known for their effects on reproductive functions. The aim was to verify the in vitro effects of 0.5-20 µM concentrations of GEN, ZEA and its metabolite α-zearalenol (α-ZOL) on pig sperm motility. A dose-dependent increase of the immotile sperm amount against fast and medium-fast sperm clusters was observed with all three oestrogens from the lowest concentrations tested. Individual CASA (computer-assisted sperm analysis) parameters of motile sperms seemed to be less sensitive indicators. This should be considered especially in toxicological research on a sperm model. Background of inconsistencies in to date-published papers is discussed. The results shift the effective concentrations of ZEA, α-ZOL, and GEN to values achievable in vivo and raises the questions of risk assessment of these compounds in pig reproduction.

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Oxidative stress and motility in tench Tinca tinca spermatozoa

Cited by 10 publications

References 25 publications

Fish Sperm Physiology: Structure, Factors Regulating Motility, and Motility Evaluation

Fish Sperm Physiology: Structure, Factors Regulating Motility, and Motility Evaluation

Equilibration and freezing interactions affecting bull sperm characteristics after thawing

Effects of zearalenone, α-zearalenol, and genistein on boar sperm motility in vitro

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