Oxidative stress and gene expression in diverse tissues of Oryzias javanicus exposed to 17β-estradiol

Woo, Seonock; Won, Hyokyoung; Lee, Aekyung; Yum, Seungshic

doi:10.1007/s13273-012-0032-6

Cited by 13 publications

(6 citation statements)

References 26 publications

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“…In this work, significant gender differences were found. Previous studies demonstrated that estradiol or endocrine-disruptor chemicals could induce the expression of antioxidant genes, such as GST , but reduce CAT and MT expression [ 24 , 28 , 64 ]. At 14 days, the GST induction relative to the controls was higher in females than males, albeit not significantly so, while the induction of MT and CAT expression was significantly higher in males.…”

Section: Discussionmentioning

confidence: 99%

“…The abnormal induction of apoptosis can cause various diseases, such as neurodegenerative and cardiovascular diseases [ 20 , 21 ]. It has been reported that caspase-3 ( Cas3 ), Cas8 , and Cas9 gene induction causes apoptosis, and several factors can influence apoptosis and antioxidant gene expression, such as gender and tissue type [ 13 , 22 – 28 ].…”

Section: Introductionmentioning

confidence: 99%

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Multiwall Carbon Nanotube-Induced Apoptosis and Antioxidant Gene Expression in the Gills, Liver, and Intestine ofOryzias latipes

Lee

Choi

Kim

et al. 2015

BioMed Research International

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Multiwall carbon nanotubes (MWCNTs) have many attractive properties with potential applications in various fields. Despite their usefulness, however, the associated waste can be hazardous to the environment. To examine adverse effects in aquatic environments, Oryzias latipes were exposed to MWCNTs dispersed in water for 14 days and apoptosis and antioxidant gene expression were observed. This work showed that in gills exposed to 100 mg/L MWCNTs for 4 days, there was significant p53, caspase-3 (Cas3), caspase-8 (Cas8), and caspase-9 (Cas9) gene expression relative to the controls, while catalase (CAT) and glutathione-S-transferase (GST) expression were reduced. At 14 days, CAT, GST, and metallothionein (MT) were induced significantly in the gills and Cas3, Cas8, and Cas9 were induced in the liver. No significant gene induction was seen in intestine. Intracellular reactive oxygen species (ROS) were increased significantly only at 14 days. Histologically, no apoptosis was observed with exposure to 100 mg/L MWCNTs for 21 days. The gills were more sensitive to MWCNT toxicity than the other organs. Males had higher apoptosis gene induction than females. These results demonstrated that MWCNTs could cause apoptosis in a manner influenced by tissue and gender in aqueous environments.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Multiwall Carbon Nanotube-Induced Apoptosis and Antioxidant Gene Expression in the Gills, Liver, and Intestine ofOryzias latipes

Lee

Choi

Kim

et al. 2015

BioMed Research International

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“…Thus, we further evaluated the formation of free radicals, and the results of the chlorophyllin assay revealed a positive correlation between the E 2 dose and the amount of free radicals ( Fig 2 ). Although E 2 inhibits oxidative stress and prevents H 2 O 2 -induced apoptosis in some mammalian cells [ 20 ], other studies have demonstrated that the exogenous addition of E 2 elevates oxidative stress in fish [ 14 ] or rat-derived cell lines [ 15 ]. This result likely reflects the degradation of E 2 to 4-OHE 2 , which subsequently undergoes redox cycling, producing reactive oxygen species (ROS) [ 19 ].…”

Section: Resultsmentioning

confidence: 99%

“…Some studies have demonstrated that the exogenous addition of E 2 increases oxidative stress in fish [ 14 ] or rat-derived cell lines [ 15 ], describing elevation of a number of indicators of oxidative stress, including metallothionein (MT) and glutathione peroxidase (GPx). Despite this fact, no data regarding this phenomenon in invertebrates are available.…”

Section: Introductionmentioning

confidence: 99%

Exposure to 17β-Oestradiol Induces Oxidative Stress in the Non-Oestrogen Receptor Invertebrate Species Eisenia fetida

et al. 2015

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BackgroundThe environmental impacts of various substances on all levels of organisms are under investigation. Among these substances, endocrine-disrupting compounds (EDCs) present a threat, although the environmental significance of these compounds remains largely unknown. To shed some light on this field, we assessed the effects of 17β-oestradiol on the growth, reproduction and formation of free radicals in Eisenia fetida.Methodology/Principal FindingsAlthough the observed effects on growth and survival were relatively weak, a strong impact on reproduction was observed (50.70% inhibition in 100 μg/kg of E2). We further demonstrated that the exposure of the earthworm Eisenia fetida to a contaminant of emerging concern, 17β-oestradiol (E2), significantly affected the molecules involved in antioxidant defence. Exposure to E2 results in the production of reactive oxygen species (ROS) and the stimulation of antioxidant systems (metallothionein and reduced oxidized glutathione ratio) but not phytochelatins at both the mRNA and translated protein levels. Matrix-assisted laser desorption/ionization (MALDI)-imaging revealed the subcuticular bioaccumulation of oestradiol-3,4-quinone, altering the levels of local antioxidants in a time-dependent manner.Conclusions/SignificanceThe present study illustrates that although most invertebrates do not possess oestrogen receptors, these organisms can be affected by oestrogen hormones, likely reflecting free diffusion into the cellular microenvironment with subsequent degradation to molecules that undergo redox cycling, producing ROS, thereby increasing environmental contamination that also perilously affects keystone animals, forming lower trophic levels.

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“…MT is primarily responsible for metal sequestration and detoxification. It was previously shown that metallothio- nein expression is modulated also by E 2 in in vivo studies using fish experimental models (30,31), where a positive correlation between MT1A expression and E 2 levels was described in muscle tissue in contrast to liver and intestines. Our results revealed that the application of E 2 -based liposomes with no cargo elevated the MT1A expression by more than 60% (90 µl of l1 added) or 100% (900 µl of l1 added), when compared with the control (Fig.…”

Section: Influence Of E 2 -Based Liposomes On Mcf-7 Cell Selected Genmentioning

confidence: 97%

17β-estradiol-containing liposomes as a novel delivery system for the antisense therapy of ER-positive breast cancer: An in vitro study on the MCF-7 cell line

et al. 2014

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The present study suggests and describes the application of a delivery system for antisense oligonucleotides against mRNA encoding estrogen receptor proteins α and β. The delivery system is composed of a cationic liposome envelope containing 17β-estradiol (E2) in its structure. Cationic liposomes protect cargo against the extracellular matrix, and E2 can increase its shuttling efficiency into cells. Using MCF-7 cells derived from estrogen receptor-positive ductal carcinoma, treatment with liposomes against ERα was found to decrease MCF-7 proliferation, and importantly the application of both the antisense against ERα and β exhibited an antiproliferative effect expressed as cell viability. Using qRT-PCR, it was shown that MT1A, NF-κB1 and K-ras genes, but not TFF1, were downregulated using E2-based liposomes (evaluated at P=0.05). Further indicators of oxidative stress were employed to assess the effect on treatment efficiency. Glutathione (GSH/GSSG redox ratio), metallothionein (MT) and malondialdehyde (MDA) confirmed a positive effect of antisense therapy resulting in their decreased levels in the MCF-7 cells. Based on these data, we suggest that E2-based liposomes offer sufficient transfer efficiency and moreover, due to the effect on NF-κB1, MT and GSH, tumor cells can be chemosensitized to increase treatment effectiveness.

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Oxidative stress and gene expression in diverse tissues of Oryzias javanicus exposed to 17β-estradiol

Cited by 13 publications

References 26 publications

Multiwall Carbon Nanotube-Induced Apoptosis and Antioxidant Gene Expression in the Gills, Liver, and Intestine ofOryzias latipes

Multiwall Carbon Nanotube-Induced Apoptosis and Antioxidant Gene Expression in the Gills, Liver, and Intestine ofOryzias latipes

Exposure to 17β-Oestradiol Induces Oxidative Stress in the Non-Oestrogen Receptor Invertebrate Species Eisenia fetida

17β-estradiol-containing liposomes as a novel delivery system for the antisense therapy of ER-positive breast cancer: An in vitro study on the MCF-7 cell line

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