Oxidative stress and DNA damage in critically ill patients with sepsis

Mihaljevic, Olgica; Zivancevic-Simonovic, Snezana; Jovanović, Danijela; Miletić-Drakulić, Svetlana; Vukajlović, Jovana Tubić; Marković, Aleksandra; Pirković, Marijanа Stanojević; Srejović, Ivan; Jakovljević, Vladimir; Milošević-Djordjević, Olivera

doi:10.1016/j.mrgentox.2023.503655

Cited by 2 publications

(8 citation statements)

References 62 publications

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“…The level of O 2 − was measured at the wavelength of maximum absorption λmax = 550 nm. The corresponding volume of distilled water was used as a blank [ 20 ].…”

Section: Methodsmentioning

confidence: 99%

“…After 10 min of incubation at room temperature, the absorbance at λ = 610 nm was measured. An equivalent volume of distilled water was used as a blank [ 20 ].…”

Section: Methodsmentioning

confidence: 99%

“…After incubation, the samples were left for 10 min at room temperature, and the measurement was performed at λ = 530 nm. An appropriate volume of distilled water was used as a blank [ 20 ].…”

Section: Methodsmentioning

confidence: 99%

“…Absorption was measured spectrophotometrically at λ = 470 nm. Distilled water was used as blank instead of blood lysate [ 20 ].…”

Section: Methodsmentioning

confidence: 99%

“…The measurement was performed six consecutive times at λ = 360 nm. An equivalent volume of distilled water was used as blank instead of blood lysate [ 20 ].…”

Section: Methodsmentioning

confidence: 99%

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Laboratory Puzzle of Oxidative Stress, Parameters of Hemostasis and Inflammation in Hospitalized Patients with COVID-19

Djordjevic,

Ignjatovic,

Vukomanovic

et al. 2024

Biomedicines

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Bearing in mind that coronavirus disease (COVID-19) is associated with a wide range of laboratory abnormalities, the aim of this study was to examine the importance of determining the parameters of oxidative stress and antioxidant protection as well as markers of inflammation and hemostasis in hospitalized patients with COVID-19. The study population included 105 patients with severe COVID-19 and 65 healthy control subjects. The parameters of oxidative stress and the activity of enzymes of the antioxidant system were determined from the obtained samples using spectrophotometric methods. Standard laboratory methods were performed for the determination of the biochemical and hematological parameters. Patients with COVID-19 showed a significantly higher level of pro-oxidative parameters (hydrogen peroxide (H2O2) and the index of lipid peroxidation in the form of thiobarbituric acid-reactive substances (TBARSs)) and a significantly lower activity of the antioxidant system (catalase (CAT)). Patients with COVID-19 had significantly higher values of inflammation parameters (C-reactive protein (CRP), procalcitonin (PCT), ratio of the number of neutrophils to lymphocytes (NLR), and ratio of the number of platelets to lymphocytes (PLR)) and parameters of hemostasis (activated partial thromboplastin time (aPTT), prothrombin time (PT), D-dimer, fibrinogen) than the control healthy subjects. In addition, changes in hemostatic parameters correlated positively with inflammatory markers in the group of patients with COVID-19. The early determination of hemostasis parameters and the parameters of inflammation can help in the prediction of poor prognosis in COVID-19 patients.

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“…The level of O 2 − was measured at the wavelength of maximum absorption λmax = 550 nm. The corresponding volume of distilled water was used as a blank [ 20 ].…”

Section: Methodsmentioning

confidence: 99%

“…After 10 min of incubation at room temperature, the absorbance at λ = 610 nm was measured. An equivalent volume of distilled water was used as a blank [ 20 ].…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…Absorption was measured spectrophotometrically at λ = 470 nm. Distilled water was used as blank instead of blood lysate [ 20 ].…”

Section: Methodsmentioning

confidence: 99%

“…The measurement was performed six consecutive times at λ = 360 nm. An equivalent volume of distilled water was used as blank instead of blood lysate [ 20 ].…”

Section: Methodsmentioning

confidence: 99%

See 3 more Smart Citations

Laboratory Puzzle of Oxidative Stress, Parameters of Hemostasis and Inflammation in Hospitalized Patients with COVID-19

Djordjevic,

Ignjatovic,

Vukomanovic

et al. 2024

Biomedicines

Self Cite

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The emerging role of mtDNA release in sepsis: Current evidence and potential therapeutic targets

Hong,

Zhu,

et al. 2024

Journal Cellular Physiology

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Sepsis is a systemic inflammatory reaction caused by infection, and severe sepsis can develop into septic shock, eventually leading to multiorgan dysfunction and even death. In recent years, studies have shown that mitochondrial damage is closely related to the occurrence and development of sepsis. Recent years have seen a surge in concern over mitochondrial DNA (mtDNA), as anomalies in this material can lead to cellular dysfunction, disruption of aerobic respiration, and even death of the cell. In this review, we discuss the latest findings on the mechanisms of mitochondrial damage and the molecular mechanisms controlling mitochondrial mtDNA release. We also explored the connection between mtDNA misplacement and inflammatory activation. Additionally, we propose potential therapeutic targets of mtDNA for sepsis treatment.

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Oxidative stress and DNA damage in critically ill patients with sepsis

Cited by 2 publications

References 62 publications

Laboratory Puzzle of Oxidative Stress, Parameters of Hemostasis and Inflammation in Hospitalized Patients with COVID-19

Laboratory Puzzle of Oxidative Stress, Parameters of Hemostasis and Inflammation in Hospitalized Patients with COVID-19

The emerging role of mtDNA release in sepsis: Current evidence and potential therapeutic targets

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