Oxidative stress adaptation in aggressive prostate cancer may be counteracted by the reduction of glutathione reductase

Freitas, Mariana; Baldeiras, Inês; Proença, Teresa; Alves, Vera; Mota-Pinto, Anabela; Sarmento‐Ribeiro, Ana Bela

doi:10.1016/j.fob.2012.05.001

Cited by 49 publications

(46 citation statements)

References 57 publications

(114 reference statements)

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“…A dose-response relationship has been observed in regards to protection from DNA damage, with increasing vitamin C benefiting those with baseline low levels (Herbert et al, 2006;Freitas et al, 2012). In our study a significant inverse association was apparent between vitamin C intake and DNA damage (p = 0.007) at study end.…”

Section: Discussionsupporting

confidence: 58%

Do New Zealand men with prostate cancer benefit from a Mediterranean-style diet?

Erdrich

Bishop

Karunasinghe

et al. 2015

Preprint

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Carcinoma of the prostate is the most commonly diagnosed malignancy and the third leading cause of mortality in New Zealand men, making it a significant health issue in this country. Global distribution patterns suggest that diet and lifestyle factors may be linked to the development and progression of this cancer. Twenty men with diagnosed prostate cancer adhered to a Mediterranean diet, with specific adaptations, for three months. Dietary data, prostate-specific antigen, C-reactive protein and DNA damage were evaluated at baseline after three months of following the diet. A significant reduction in DNA damage compared to baseline was apparent, with particular benefit noted for overall adherence to the diet (p = 0.013), increased intake of folate (p = 0.023), vitamin C (p = 0.007), legumes (p = 0.004) and green tea (p = 0.002). Higher intakes of red meat and dairy products were inversely associated with DNA damage (p = 0.003 and p = 0.008 respectively). This small study demonstrated that a high-antioxidant diet, modelled on Mediterranean traditions, may be of benefit for men with prostate cancer. Protection against DNA damage appears to be associated with the diet implemented, ostensibly due to reduction in reactive oxidant species. These findings warrant further exploration in a longer trial, with a larger cohort.

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Section: Discussionsupporting

confidence: 58%

Do New Zealand men with prostate cancer benefit from a Mediterranean-style diet?

Erdrich

Bishop

Karunasinghe

et al. 2015

Preprint

View full text Add to dashboard Cite

show abstract

“…The role of vitamin C in regards to cancer is conflicting (Fraga et al, 1991;Herbert et al, 2006) making the finding of this study difficult to compare with other research. A dose-response relationship has been observed in regards to protection from DNA damage, with increasing vitamin C benefiting those with baseline low levels (Herbert et al, 2006;Freitas et al, 2012). In our study a significant inverse association was apparent between vitamin C intake and DNA damage (p = 0.007) at study end.…”

Section: Discussionsupporting

confidence: 58%

Do New Zealand men with prostate cancer benefit from a Mediterranean-style diet?

Erdrich

Bishop

Karunasinghe

et al. 2015

Preprint

View full text Add to dashboard Cite

Carcinoma of the prostate is the most commonly diagnosed malignancy and the third leading cause of mortality in New Zealand men, making it a significant health issue in this country. Global distribution patterns suggest that diet and lifestyle factors may be linked to the development and progression of this cancer. Twenty men with diagnosed prostate cancer adhered to a Mediterranean diet, with specific adaptations, for three months.Dietary data, prostate-specific antigen, C-reactive protein and DNA damage were evaluated at baseline after three months of following the diet. A significant reduction in DNA damage compared to baseline was apparent, with particular benefit noted for overall adherence to the diet (p = 0.013), increased intake of folate (p = 0.023), vitamin C (p = 0.007), legumes (p = 0.004) and green tea (p = 0.002). Higher intakes of red meat and dairy products were inversely associated with DNA damage (p = 0.003 and p = 0.008 respectively). This small study demonstrated that a high-antioxidant diet, modelled on Mediterranean traditions, may be of benefit for men with prostate cancer. Protection against DNA damage appears to be associated with the diet implemented, ostensibly due to reduction in reactive oxidant species. These findings warrant further exploration in a longer trial, with a larger cohort.PeerJ PrePrints | http://dx.doi.org/10.7287/peerj.preprints.783v1 | CC-BY 4.0 Open Access | rec

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“…Therefore it is possible that these thiol metabolites contribute to the remaining free thiol in LNCaP cells. Other reporters have also indicated that glutathione increase in response to increased ROS in metastatic cell lines [38,39], and that it contributes to a reduced percentage of the total free thiol in LNCaP cells [40].…”

Section: Figurementioning

confidence: 99%

Determination of cellular glutathione:glutathione disulfide ratio in prostate cancer cells by high performance liquid chromatography with electrochemical detection

Childs

Haroune

Williams

et al. 2016

Journal of Chromatography A

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A validated method has been developed for the simultaneous measurement of reduced and oxidized glutathione in de-proteinised cellular extracts. This has been used to compare models of malignant and non-malignant human prostate cell lines. Analysis of LNCaP and DU145 cells showed a glutathione to glutathione disulfide ratio of 8:1 and 32:1 respectively, whilst the control cell line, PZ-HPV7 displayed a ratio of 93:1. Results indicate that the more aggressive phenotype displays adaptation to increased oxidative stress via up regulation of glutathione turnover. It was also noted that in the LNCaP and DU145 cell line, glutathione was only responsible for ca. 60% and 79% respectively, of the total cellular reduced thiol; indicating the presence of other biological thiols.

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Oxidative stress adaptation in aggressive prostate cancer may be counteracted by the reduction of glutathione reductase

Cited by 49 publications

References 57 publications

Do New Zealand men with prostate cancer benefit from a Mediterranean-style diet?

Do New Zealand men with prostate cancer benefit from a Mediterranean-style diet?

Do New Zealand men with prostate cancer benefit from a Mediterranean-style diet?

Determination of cellular glutathione:glutathione disulfide ratio in prostate cancer cells by high performance liquid chromatography with electrochemical detection

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