Determination of cellular glutathione:glutathione disulfide ratio in prostate cancer cells by high performance liquid chromatography with electrochemical detection

Childs, Stephen; Haroune, Nicolas; Williams, Lisa-Mae; Gronow, Michael

doi:10.1016/j.chroma.2016.01.050

Cited by 34 publications

(18 citation statements)

References 38 publications

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“…The results demonstrated that the more aggressive phenotype of prostate cancer cells shows adaptation to increased oxidative stress via up-regulation of glutathione turnover [32]. To the extent of our knowledge, there is no literature evaluating the relationship between thiol and PSA levels.…”

Section: Discussionmentioning

confidence: 94%

Dynamic thiol/disulphide homeostasis before and after radical prostatectomy in patients with prostate cancer

Hanikoglu¹,

Hanikoğlu

Kucuksayan

et al. 2016

Free Radical Research

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Thiol groups are important anti-oxidants and essential molecules protecting organism against the harmful effects of reactive oxygen species (ROS). The aim of our study is to evaluate thiol-disulphide homeostasis with a novel recent automated method in patients with localized prostate cancer (PC) before and six months after radical prostatectomy (RP). 18 patients with PC and 17 healthy control subjects were enrolled into the study. Blood samples were collected from the controls subjects and patients before and six months after RP. Thiol-disulphide homeostasis was determined using a recently developed novel method. Prostate-specific antigen (PSA), albumin, total protein, total thiol, native thiol, disulphide and total antioxidant status (TAS) were measured and compared between the groups. Native thiol, total thiol and TAS levels were significantly higher in the control group than the patients before RP (p < .001). There was a non-significant increase in the native thiol, total thiol and TAS levels in the patients six months after RP in comparison to the levels before RP (p values .3, .3 and .09, respectively). We found a significant negative correlation between PSA and thiol levels. Our study demonstrated that the decreased thiol and TAS levels weakened anti-oxidant defence mechanism in the patients with PC as indicated. Increased oxidative stress in prostate cancer patients may cause metabolic disturbance and have a role in the aetiopathogenesis of prostate cancer. ARTICLE HISTORY

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Section: Discussionmentioning

confidence: 94%

Dynamic thiol/disulphide homeostasis before and after radical prostatectomy in patients with prostate cancer

Hanikoglu¹,

Hanikoğlu

Kucuksayan

et al. 2016

Free Radical Research

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“…The amount of GSH present in the ASF was higher than previously found; possibly due to 2 hours re-incubation of confluent cells in fresh medium employed before harvesting. However the presence of significant quantities of cysteine (which usually quickly incorporated into GSH) and cysteinylglycine indicates that abnormal/aberrant GSH metabolic pathways exist in this tumor cell line [19]. In the normal cellular synthesis of GSH the only cysteinyl dipeptide involved is γ-glutamylcysteine; GSH synthetase adds glycine to this to give the tripeptide.…”

Section: Discussionmentioning

confidence: 99%

Investigation of the Low Molecular Weight Thiol Composition in a Metastatic Prostate Cancer Cell Line (LNCaP) by LC-UV-MS and NMR after Labelling with the Ellman Reagent

Childs¹,

Haroune²,

Williams³

et al. 2017

AJAC

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The low molecular weight thiols present in the deproteinized extract of a prostate cancer cell line (LNCaP-FGC) were analysed after derivatization with the Ellman reagent (ESSE). The mixed disulphides formed (RSSE) were fractionated, characterized and quantified by liquid chromatography on a C-18 column using UV detection. This revealed the presence, in femtomoles per cell, of glutathione (8.30 ± 0.73), cysteine (2.71 ± 0.04) and cysteinylglycine (0.83 ± 0.10), accounting for the bulk of the thiol present. Further analysis of the cell extracts using a novel and sensitive mass spectrometry technique allowed the detection of low level of an additional derivative which was identified as cysteinylglycerate using NMRspectroscopy.

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“…To date, a number of analytical methods have been established for GSH measurement, including high-performance liquid chromatography [5], mass spectrometry [6], surface enhanced Raman scattering [7], enzyme-linked immunosorbent assay [8], electrochemical analysis [9], and fluorescence spectroscopy [10]. Fluorescence spectrometry has been found to be a powerful tool for GSH detection because of its simplicity, high sensitivity, good flexibility, and nondestructive readout [11,12].…”

Section: Introductionmentioning

confidence: 99%

A Fluorescence Inner-Filter Effect Based Sensing Platform for Turn-On Detection of Glutathione in Human Serum

Tang

You

Fang

et al. 2019

Sensors

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A novel turn-on fluorescence assay was developed for the rapid detection of glutathione (GSH) based on the inner-filter effect (IFE) and redox reaction. Molybdenum disulfide quantum dots (MoS2 QDs), which have stable fluorescent properties, were synthesized with hydrothermal method. Manganese dioxide nanosheets (MnO2 NSs) were prepared by exfoliating the bulk δ-MnO2 material in bovine serum albumin (BSA) aqueous solution. The morphology structures of the prepared nanoparticles were characterized by transmission electron microscope (TEM). Studies have shown that the fluorescence of MoS2 QDs could be quenched in the presence of MnO2 NSs as a result of the IFE, and is recovered after the addition of GSH to dissolve the MnO2 NSs. The fluorescence intensity showed a good linear relationship with the GSH concentration in the range 20–2500 μM, the limit of detection was 1.0 μM. The detection method was applied to the analysis of GSH in human serum samples. This simple, rapid, and cost-effective method has great potential in analyzing GSH and in disease diagnosis.

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Determination of cellular glutathione:glutathione disulfide ratio in prostate cancer cells by high performance liquid chromatography with electrochemical detection

Cited by 34 publications

References 38 publications

Dynamic thiol/disulphide homeostasis before and after radical prostatectomy in patients with prostate cancer

Dynamic thiol/disulphide homeostasis before and after radical prostatectomy in patients with prostate cancer

Investigation of the Low Molecular Weight Thiol Composition in a Metastatic Prostate Cancer Cell Line (LNCaP) by LC-UV-MS and NMR after Labelling with the Ellman Reagent

A Fluorescence Inner-Filter Effect Based Sensing Platform for Turn-On Detection of Glutathione in Human Serum

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