Oxidative processes during enzymatic hydrolysis of cod protein and their influence on antioxidant and immunomodulating ability

Halldorsdottir, Sigrun M.; Sveinsdóttir, Herdís; Freysdóttir, Jona; Kristinsson, Hörður G.

doi:10.1016/j.foodchem.2013.07.053

Cited by 24 publications

(20 citation statements)

References 25 publications

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“…The oxygen radical absorbance capacity (ORAC) assay was performed according to Halldorsdottir et al (2014). The samples with the concentration range of 0.3-0.7 mg/mL were used in the assay and the measurement of area under the fluorescence decay curve was conducted using POLARstar Optima microplate reader (BMG Labtech, Offenburg, Germany).…”

Section: Chemical Antioxidant Activitiesmentioning

confidence: 99%

“…Cell ROS determination by DCFH-DA Intracellular formation of ROS was assessed using an oxidation sensitive dye DCFH-DA as the substrate according to the method of Halldorsdottir et al (2014) with some modification. HepG2 cells (6×10 4 cells/well) seeded in black 96-well plates were loaded with 100 μL DCFH-DA (1 μM in Hank's Balanced Salt Solution, HBSS) and incubated in the dark for 30 min.…”

Section: Cytotoxicity Effect Of H 2 O 2 or Aaph On Hepg2 Cellsmentioning

confidence: 99%

“…The result suggested that HepG2 cells were sensitive to H 2 O 2 and peroxyl radical, especially when the level was higher than 200 μM (P<0.05). In general, ROS can cause oxidative stress and damage of biomolecules in the cell, leading to cell death and serious chronic diseases (Halldorsdottir et al 2014). Zhang et al (2012) reported that the use of H 2 O 2 (50-200 μM) exhibited a dose dependent decrease in PC12 cell viability.…”

Section: Effect Of Oxidative Stressors On Cytotoxicity Of Hepg2 Cellmentioning

confidence: 99%

“…Numerous antioxidant compounds, particularly fish peptide, have been widely studied. Potent antioxidant peptides have been isolated from protein hydrolysates from the muscle of yellow stripe trevally (Selaroides leptolepis) (Klompong et al 2009), cod (Gadus morhua) (Halldorsdottir et al 2014) and spotless smooth hound (Mustelus griseus) (Wang et al 2014), etc. Protein, peptides and free amino acids released during enzymatic hydrolysis are capable of modulating specific biological function.…”

Section: Introductionmentioning

confidence: 99%

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Preventive effect of Nile tilapia hydrolysate against oxidative damage of HepG2 cells and DNA mediated by H2O2 and AAPH

et al. 2015

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Antioxidant activities of protein hydrolysate prepared from Nile tilapia protein isolate using Alcalase (HA), Alcalase followed by papain (HAPa) and their Sephadex G-25 fractions (FHA and FHAPa) were investigated in both chemical and cellular based models. Amongst all samples, FHAPa showed the highest chemical antioxidant activities, however it had no metal chelation activity. Cellular antioxidant ability of HA, HAPa and their fractions against H 2 O 2 and AAPH induced oxidative damage of HepG2 cell and DNA were tested. When cells were pretreated with all hydrolysates or fractions at different concentrations (0.5-2 mg/mL) in the absence and presence of 50 μM Trolox, cell viability was in the range of 91.10-111.40 %. However, no difference in cell viability was observed among samples having various concentrations (P>0.05). Cell reactive oxygen species (ROS) generation as mediated by H 2 O 2 and AAPH decreased with treatment of hydrolysates or their fractions, especially in combination with 50 μM Trolox. FHAPa effectively inhibited H 2 O 2 and peroxyl radical induced DNA scission in a dose dependent manner. Therefore, Nile tilapia protein hydrolysates could serve as a functional food ingredient.

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Section: Chemical Antioxidant Activitiesmentioning

confidence: 99%

Section: Cytotoxicity Effect Of H 2 O 2 or Aaph On Hepg2 Cellsmentioning

confidence: 99%

Section: Effect Of Oxidative Stressors On Cytotoxicity Of Hepg2 Cellmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Preventive effect of Nile tilapia hydrolysate against oxidative damage of HepG2 cells and DNA mediated by H2O2 and AAPH

et al. 2015

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“…Immunomodulatory peptides can enhance immune cell functions such as lymphocyte proliferation, natural killer (NK) cell activity, antibody synthesis, and cytokine regulation (Singh et al, 2014). Many immunomodulatory peptides have been isolated from enzymatic digests of various food proteins such as those found in fish, soy, and whey (Halldorsdottir et al, 2014;Kong et al, 2008;Saint-Sauveur et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Production optimization and characterization of immunomodulatory peptides obtained from fermented goat placenta

Hou¹,

Liu²,

Cheng³

et al. 2014

Food Sci. Technol (Campinas)

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Effect ofin vitrodigested cod liver oil of different quality on oxidative, proteomic and inflammatory responses in the yeastSaccharomyces cerevisiaeand human monocyte-derived dendritic cells

Larsson

Istenič

Wulff

et al. 2015

J. Sci. Food Agric.

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show abstract

Oxidative processes during enzymatic hydrolysis of cod protein and their influence on antioxidant and immunomodulating ability

Cited by 24 publications

References 25 publications

Preventive effect of Nile tilapia hydrolysate against oxidative damage of HepG2 cells and DNA mediated by H2O2 and AAPH

Preventive effect of Nile tilapia hydrolysate against oxidative damage of HepG2 cells and DNA mediated by H2O2 and AAPH

Production optimization and characterization of immunomodulatory peptides obtained from fermented goat placenta

Effect ofin vitrodigested cod liver oil of different quality on oxidative, proteomic and inflammatory responses in the yeastSaccharomyces cerevisiaeand human monocyte-derived dendritic cells

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