Oxidative parameters of embryo culture media may predict treatment outcome in in vitro fertilization: a novel applicable tool for improving embryo selection

Wiener-Megnazi, Zofnat; Shiloh, Hanna; Avraham, Limor; Lahav-Baratz, Shirly; Koifman, Mara; Reznick, Abraham Z.; Auslender, Ron; Dirnfeld, Martha

doi:10.1016/j.fertnstert.2010.10.019

Cited by 15 publications

(15 citation statements)

References 37 publications

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“…The embryos of smoking patients may undergo more oxidative processes, resulting in higher oxidative levels in the cryopreservation medium. This finding is in line with our previous study assessing TCL oxidative parameters in embryo culture medium [16] and may reflect the maternally derived environmental influence on in vitro cultured embryos. Thus, we speculate that embryos from smoking patients Bsense^their mother's smoking status.…”

Section: Discussionsupporting

confidence: 92%

“…In our previous work [16] evaluating oxidative parameters in embryo culture media from IVF, the TCL H1 amplitude was the most predictive parameter for treatment success. Therefore, in this study evaluating oxidative parameters in embryo cryopreservation medium, we assumed that the TCL H1 amplitude may also potentially impact treatment outcome.…”

Section: Discussionmentioning

confidence: 94%

“…In a previous study [15], we found an association between oxidative parameters in follicular fluid as measured by the TCL assay, ovarian reserve parameters and the ability to conceive. In another study [16], we found that TCL oxidative parameters in the embryo culture media may serve as quick, simple, and accurate tools for embryo selection in IVF. The objective of the present study was to evaluate the possible association between TCL oxidative parameters in embryo cryopreservation medium and laboratory and clinical parameters of treatment success.…”

Section: Introductionmentioning

confidence: 94%

“…The TCL Analyzer is a sensitive, simple, and fast (7 min) method for assessing oxidizability in a given sample of biological fluid. Its convenient size (30 cm × 35 cm × 40 cm) enables its use in an IVF laboratory setup, and sample volumes as small as 10 μL can be analyzed [16]. In a previous study [15], we found an association between oxidative parameters in follicular fluid as measured by the TCL assay, ovarian reserve parameters and the ability to conceive.…”

Section: Introductionmentioning

confidence: 94%

“…However, these changes are currently assessed only by classical morphological grading and blastomere survival. The thermochemiluminescence (TCL) assay developed in 1994 by Shnizer et al has been used to investigate oxidative stress in biological fluids [11] and was validated in several studies using various biological samples such as the following: bovine serum albumin, polyunsaturated fatty acids [11], human and rat blood serum [12,13], human seminal plasma [14], human bronchoalveolar lavage fluid, amniotic fluid, follicular fluid [15], and embryo culture media [16].…”

Section: Introductionmentioning

confidence: 99%

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Oxidative markers in cryopreservation medium from frozen-thawed embryos: a possible tool for improved embryo selection in in vitro fertilization?

Wiener-Megnazi

Lahav-Baratz

Blais

et al. 2016

J Assist Reprod Genet

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Purpose The present study evaluated the association between oxidative parameters in embryo cryopreservation medium and laboratory and clinical outcomes. Methods This prospective laboratory study was conducted in an IVF unit in a university-affiliated hospital with 91 IVF patients undergoing a frozen-thawed embryo transfer cycle. Following thawing, 50 μL of embryo cryopreservation medium was retrieved from each cryotube and tested by the thermochemiluminescence (TCL) assay. TCL amplitudes after 50 (H1), 150 (H2), and 280 s (H3) were recorded in counts per second (CPS) and the TCL ratio determined for comparison with implantation and pregnancy rates. Results A total of 194 embryos were transferred in 85 frozenthaw cycles. Twenty-one pregnancies (24.7 %) occurred. Implantation and overall and clinical pregnancy rates were higher when the median TCL H1 amplitude was <32 CPS compared to ≥32 CPS (14.6 vs. 5.3 %, 37.5 vs. 17 %, 28.1 vs. 9.4 %, respectively). No pregnancies occurred when the H1 amplitude was ≥40 CPS. Logistic regression multivariate analysis found that only the median TCL H1 amplitude was associated with the occurrence of pregnancy (OR = 2.93, 95 % CI 1.065-8.08). The TCL ratio inversely correlated with the duration of embryo cryopreservation (r = −0.37).Conclusions The results indicate that thawed embryos may express oxidative processes in the cryopreservation medium, and higher oxidative levels are associated with lower implantation rates. These findings may aid in the improved selection of frozen-thawed embryos for IVF.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 94%

Section: Introductionmentioning

confidence: 94%

Section: Introductionmentioning

confidence: 94%

Section: Introductionmentioning

confidence: 99%

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Oxidative markers in cryopreservation medium from frozen-thawed embryos: a possible tool for improved embryo selection in in vitro fertilization?

Wiener-Megnazi

Lahav-Baratz

Blais

et al. 2016

J Assist Reprod Genet

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Synthesis of 1,2‐Dioxetanes as Thermochemiluminescent Labels for Ultrasensitive Bioassays: Rational Prediction of Olefin Photooxygenation Outcome by Using a Chemometric Approach

Andronico

Quintavalla

Lombardo

et al. 2016

Chemistry A European J

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Great interest in new thermochemiluminescent (TCL) molecules, for example, in bioanalytical assays, has prompted the design and synthesis of a small library of more than 30 olefins to be subjected to photooxygenation, with the aim of obtaining new 1,2-dioxetane-based TCL labels with optimized properties. Fluorine atoms on the acridan system remarkably stabilize 1,2-dioxetanes when they are located in the 3- and/or 6-position (4 h and 4 i). On the other hand, 2,7-difluorinated acridan dioxetane (4 j) showed a significantly enhanced fluorescence quantum yield with respect to the unsubstituted dioxetane (4 a). Some of the synthesized olefins did not undergo singlet oxygen addition and a rationale was sought to ease the photooxygenation step, leading to the TCL dioxetanes. A chemometric approach has been adopted to exploit principal component analysis and linear discriminant analysis of the structural and electronic molecular descriptors obtained by DFT optimizations of olefins 3. This approach allows the steric and electronic parameters that govern dioxetane formation to be revealed.

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Effect of Oxidative Stress on ART Outcome

Hammadeh

Hamad

Refaat

et al. 2012

Studies on Men's Health and Fertility

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Oxidative parameters of embryo culture media may predict treatment outcome in in vitro fertilization: a novel applicable tool for improving embryo selection

Cited by 15 publications

References 37 publications

Oxidative markers in cryopreservation medium from frozen-thawed embryos: a possible tool for improved embryo selection in in vitro fertilization?

Oxidative markers in cryopreservation medium from frozen-thawed embryos: a possible tool for improved embryo selection in in vitro fertilization?

Synthesis of 1,2‐Dioxetanes as Thermochemiluminescent Labels for Ultrasensitive Bioassays: Rational Prediction of Olefin Photooxygenation Outcome by Using a Chemometric Approach

Effect of Oxidative Stress on ART Outcome

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