Effect of Oxidative Stress on ART Outcome

Hammadeh, Mohamad Eid; Hamad, Mohammed; Refaat, Khaled; Said, Tamer M.; Fischer-Hammadeh, C.

doi:10.1007/978-1-61779-776-7_21

Cited by 2 publications

(2 citation statements)

References 207 publications

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“…Furthermore, heavy metals and metalloids can induce oxidative stress which generate reactive oxygen species (ROS) ( 58 ). ROS generate peroxidation products which can cause depletion of antioxidant systems designed to protect the sperm from oxidative reactions and deterioration of sperm cells ( 59 , 60 ). High levels of redox activity and aneuploidy rates due to heavy metals and metalloids exposure have been detected in defective spermatozoa and failure on oocyte interaction ( 61 , 62 ).…”

Section: Discussionmentioning

confidence: 99%

Heavy metals and metalloids exposure and in vitro fertilization: Critical concerns in human reproductive medicine

Obasi

Frazzoli

Orisakwe

2022

Front. Reprod. Health

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Exposures to heavy metals and metalloids have been associated with decreased fecundity and fertility in couples conceiving via assisted reproduction. Heavy metals and metalloids can alter the homeostasis of critical hormones controlling sexual maturation by binding to critical hormones and receptors. This may disrupt the time course of sexual maturation directly or indirectly affecting reproductive competence in males and females. The present review aims to provide a summarized overview of associations between heavy metal exposure, reproductive concerns, and IVF outcomes. A systematic review was conducted according to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) in Google Scholar, Scopus, EMBASE and PubMed databases. Initial search produced 1,351 articles from which 30 articles were eligible to be included in the systematic review. From our results, 16 articles reported associations between selected heavy metals and IVF outcomes, while 14 articles summarized the role of heavy metals in reproductive concerns. For the studies on IVF outcomes, different human samples were examined for heavy metals. Heavy metals and metalloids (Pb, Hg, Cd, Cr, Mn, As) correlated negatively with oocyte fertilization/pregnancy rates in hair, follicular fluid, serum, urine and seminal plasma samples, while Cd and Hg in whole blood samples showed no associations. For the studies on reproductive concerns, high levels of heavy metals/metalloids were implicated in the following conditions: infertility (Cd, Pb, Ba, U), spontaneous abortion/miscarriage (Pb, Cd, Sb), congenital heart disease (Al, Mg, Cd), PCOS (As, Cd, Hg, Pb), endometriosis (Pb) and uterine leiomyomata (Hg). Taken together, the results of our study suggest that the impact of heavy metals and metalloids exposure on reproductive health may contribute to the failure rates of in vitro fertilization.

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Section: Discussionmentioning

confidence: 99%

Heavy metals and metalloids exposure and in vitro fertilization: Critical concerns in human reproductive medicine

Obasi

Frazzoli

Orisakwe

2022

Front. Reprod. Health

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“…The imbalance of ROS and antioxidants will trigger metabolic disorders, damage to the spermatozoa membrane so that the spermatozoa will become immotile to death. If more and more sperm are immotile and die every hour, the higher the ROS level, the more immotile and dead sperm will produce 10-15 times more ROS than motile sperm (Bourne 2009, Pyrzak 2010, Marques et al 2014, Hammadeh et al 2009. It was seen in the results of the study that progressive motility in the sperm preparation group without ALA was worse than the sperm preparation group with the addition of ALA, and it was also seen that the difference widened with increasing time.…”

Section: Discussionmentioning

confidence: 99%

Effect of In-Vitro Alpha Lipoic Acid Addition on Spermatozoa Motility in Sperm Preparation Process

Buanayuda¹,

Lunardhi²,

Mansur³

2020

FMI

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Infertility is a problem for husband and wife, in the last 20 years the number of infertile couples has tended to increase by around 6.5 million pairs. The infertile couple can use the intrauterine insemination method to obtain offspring if a conventional method approach cannot be performed. Insemination requires a sperm preparation stage in which there are centrifugation and resuspension procedures that tend to produce excess reactive oxygen species (ROS). Excessive ROS will damage the motility of the spermatozoa. This study aims to prove the addition of alpha lipoic acid (ALA) as an antioxidant in the process of sperm preparation to improve and maintain better sperm motility. This research is a laboratory study with an experimental research design. The sample consisted of 10 infertile men who visited the Andrology section of the Sayyidah Jakarta Mother and Child Hospital (RSIA), where each ejaculate from the patient would be divided into 3 groups namely (k1) fresh semen as a control group, (k2) sperm preparation group without ALA, (k3) group of sperm preparation with the addition of ALA. The motility of spermatozoa was observed with the WHO 1999 method for 4 hours in units of percent. Progressive motility in k3 (47.95 ± 3.617) was higher than in k2 (38.05 ± 3.278) statistically significantly different after 3 hours of observation (p<0.0001). Progressive motility in k3 (78.8 ± 5.841) was higher than k1 (56.55 ± 7.511) from the initial observation (p <0.0001). The progressive motility of k2 (76.05 ± 6.768) was higher than k1 (56.55 ± 7.511) from the start of the observation (0.0001). It can be concluded that the addition of ALA in the sperm preparation process increases and maintains progressive motility that is better than sperm preparation without ALA addition after 3 hours of observation.

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Effect of Oxidative Stress on ART Outcome

Cited by 2 publications

References 207 publications

Heavy metals and metalloids exposure and in vitro fertilization: Critical concerns in human reproductive medicine

Heavy metals and metalloids exposure and in vitro fertilization: Critical concerns in human reproductive medicine

Effect of In-Vitro Alpha Lipoic Acid Addition on Spermatozoa Motility in Sperm Preparation Process

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