Oxidative DNA damage in chronic renal failure patients

Stoyanova, Elitsa; Sandoval, Silvia Berenice; Zúñiga, L.; Yamani, Naouale El; Coll, Elisabet; Pastor, Susana; Reyes, Joselyn; Enrique, Andrés; Ballarín, J.; Xamena, N.; Marcos, Ricard

doi:10.1093/ndt/gfp575

Cited by 51 publications

(41 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The observed genetic damage may also be contributed by increased serum creatinine levels (which were 2.5x higher). Stoyanova et al [32] had also observed a significant positive correlation between serum creatinine levels and genomic damage being substantiated by observations of the present study.…”

Section: Discussionsupporting

confidence: 91%

DNA damage in a patient with Systemic Lupus Erythematosus and Nephropathy- A Case Report

Tung

Gandhi

2015

Int Jour of Biomed Res

View full text Add to dashboard Cite

Objectives: Systemic Lupus Erythematosus (SLE) is a complex, multisystem autoimmune inflammatory disease. The inflammatory response in SLE exacerbates oxidative stress which tends to promote lipid peroxidation, protein oxidation and damage to DNA. Documentation of manifestation of DNA damage in SLE patients is scarce and related studies have not come to attention from this part of the world, therefore the present study showcases a case report on SLE. Methods: Assessment of DNA damage in peripheral blood leukocytes and oxidative stress biomarkers in blood sera of a 12y-old male presenting with SLE and nephropathy (on dialysis therapy) was made. Results: Basal DNA damage in PBL scored as percent DNA in tail was higher (51.48%) compared to levels (40.38%) in an age-and sex-matched healthy control. Quantitative measures of DNA damage revealed DF of 96 vs. 94 and DI of 317 vs. 208 in the case and the control, respectively. Serum lipid peroxidation as estimated from MDA level was 3x higher (1.759µmol/l) compared to its level in a healthy control (0.571µmol/l). OSI was slightly higher in the patient (0.134 arbitrary units) compared to that in the control (0.132 arbitrary units). The atherogenic indices were higher in the present case compared to ratios in the control. Conclusion:The results from the case report on increased oxidative stress, dyslipidemia, genetic damage and atherogenic indices support observations from earlier studies and imply onset of other complications in patients with SLE. The assessment of various blood-based biomarkers (as carried out in the present case) in SLE patients may assist in disease diagnosis, prognosis and optimal disease management.

show abstract

Section: Discussionsupporting

confidence: 91%

DNA damage in a patient with Systemic Lupus Erythematosus and Nephropathy- A Case Report

Tung

Gandhi

2015

Int Jour of Biomed Res

View full text Add to dashboard Cite

show abstract

“…DNA breaks present in peripheral blood lymphocytes were measured using the comet assay performed following the standard protocol, as previously described (Singh et al, 1988;Stoyanova et al, 2010) with minor modifications. Briefly, isolated lymphocytes from 2 mL of blood from each patient were cryopreserved until use, in 500 µL of medium containing 90% serum and 10% DMSO.…”

Section: Comet Assaymentioning

confidence: 99%

Unfermented grape juice reduce genomic damage on patients undergoing hemodialysis

Corredor

Rodríguez-Ribera

Coll

et al. 2016

Food and Chemical Toxicology

View full text Add to dashboard Cite

“…Several lines of evidence indicate that nucleic acid damage associated with oxidative stress increases in CKD and HD patients. Stoyanova et al [102] reported recently that oxidative nucleic acid damage was higher in HD ( n = 77) than in CKD patients ( n = 176). Another study reported that patients with CKD exhibit upregulation of a number of genes involved in the oxidative phosphorylation system, suggesting that an impaired mitochondrial respiratory system contributes to increased oxidative stress [103].…”

Section: Consequences Of Oxidative Stress and Oxidative Nucleic Acmentioning

confidence: 99%

Oxidative Stress and Nucleic Acid Oxidation in Patients with Chronic Kidney Disease

Sung

Hsu

Chen

et al. 2013

Oxidative Medicine and Cellular Longevity

157

110

View full text Add to dashboard Cite

Patients with chronic kidney disease (CKD) have high cardiovascular mortality and morbidity and a high risk for developing malignancy. Excessive oxidative stress is thought to play a major role in elevating these risks by increasing oxidative nucleic acid damage. Oxidative stress results from an imbalance between reactive oxygen/nitrogen species (RONS) production and antioxidant defense mechanisms and can cause vascular and tissue injuries as well as nucleic acid damage in CKD patients. The increased production of RONS, impaired nonenzymatic or enzymatic antioxidant defense mechanisms, and other risk factors including gene polymorphisms, uremic toxins (indoxyl sulfate), deficiency of arylesterase/paraoxonase, hyperhomocysteinemia, dialysis-associated membrane bioincompatibility, and endotoxin in patients with CKD can inhibit normal cell function by damaging cell lipids, arachidonic acid derivatives, carbohydrates, proteins, amino acids, and nucleic acids. Several clinical biomarkers and techniques have been used to detect the antioxidant status and oxidative stress/oxidative nucleic acid damage associated with long-term complications such as inflammation, atherosclerosis, amyloidosis, and malignancy in CKD patients. Antioxidant therapies have been studied to reduce the oxidative stress and nucleic acid oxidation in patients with CKD, including alpha-tocopherol, N-acetylcysteine, ascorbic acid, glutathione, folic acid, bardoxolone methyl, angiotensin-converting enzyme inhibitor, and providing better dialysis strategies. This paper provides an overview of radical production, antioxidant defence, pathogenesis and biomarkers of oxidative stress in patients with CKD, and possible antioxidant therapies.

show abstract

Oxidative DNA damage in chronic renal failure patients

Cited by 51 publications

References 39 publications

DNA damage in a patient with Systemic Lupus Erythematosus and Nephropathy- A Case Report

DNA damage in a patient with Systemic Lupus Erythematosus and Nephropathy- A Case Report

Unfermented grape juice reduce genomic damage on patients undergoing hemodialysis

Oxidative Stress and Nucleic Acid Oxidation in Patients with Chronic Kidney Disease

Contact Info

Product

Resources

About