Oxidative DNA cleavage mediated by a new unexpected [Pd(BAPP)][PdCl₄] complex (BAPP = 1,4-bis(3-aminopropyl)piperazine): crystal structure, DNA binding and cytotoxic behavior

Abstract

Synthesis, structural characterization, docking simulation, theoretical calculations, and biological evaluation of a new palladium(ii) complex have been reported.

Brief Research on the Biophysical Study and Anticancer Behavior of Pt(II) Complexes: Their DNA/BSA Binding, Molecular Docking, and Cytotoxic Property

“…The following Wolfe–Shimer equation is employed to determine the value of the binding constant K b . false[ DNA false] ε normala − ε normalf = false[ DNA false] ε normalb − ε normalf − 1 K normalb ( ε b − ε f ) where [DNA] denotes the concentration of DNA, ε a = A observed /[complex], ε b describes the free complex extinction coefficient, and ε f is the extinction coefficient when the complex is fully bound with DNA. The binding constants K b are calculated from the slope/intercept ratio of the [DNA]/(ε a – ε f ) versus [DNA] plot.…”

Water soluble transition metal [Ni(ii), Cu(ii) and Zn(ii)] complexes of N-phthaloylglycinate bis(1,2-diaminocyclohexane). DNA binding, pBR322 cleavage and cytotoxicity

“…In addition, different ROS scavenging agents viz. , hydroxyl (˙OH) radical scavengers (DMSO, EtOH), a superoxide (O 2 ˙ − ) radical scavenger (SOD) and a singlet oxygen ( 1 O 2 ) scavenger (NaN 3 ) were used to authenticate the type of cleavage exhibited by the complexes ( 1–3 ) 61,62 (Fig. 12).…”

Anticancer Behavior of Pyrrolidine-Based Palladium(II) Complexes and Biophysical Approach on Their DNA, BSA Binding Activity, Molecular Docking, and DFT Study

“…23,24 The complex concentration was kept constant (20 μM), with the variation of DNA concentration (20−200 μM) for measurement of absorption. The Wolfe−Shimer equations (eqs S1 and S2) were used to calculate the intrinsic binding constant (k b ) 25 Ethidium bromide (EtBr) is a standard fluorescent probe for DNA−EtBr displacement experiment to investigate the mode of binding and their binding constants with the metal complex. 26 A standard protocol was followed to observe the fluorescence intensity of different solutions of the DNA−EtBr adduct.…”

“…To prepare the DNA solution, a standard protocol was followed. , The complex concentration was kept constant (20 μM), with the variation of DNA concentration (20–200 μM) for measurement of absorption. The Wolfe–Shimer equations (eqs S1 and S2) were used to calculate the intrinsic binding constant ( k b ) and Gibbs free energy (Δ G ), respectively.…”