Oxidative damages in erythrocytes of patients with metabolic syndrome

Abstract

The aim of the study was to estimate the changes caused by oxidative stress in structure and function of membrane of erythrocytes from patients with metabolic syndrome (MS). The study involved 85 patients with MS before pharmacological treatment and 75 healthy volunteers as a control group. Cholesterol level, lipid peroxidation, glutathione level (GSH), and antioxidant enzyme activities in erythrocytes were investigated. The damage to erythrocyte proteins was also indicated by means of activity of ATPase (total and Na+,K+ ATPase) and thiol group level. The membrane fluidity of erythrocytes was estimated by the fluorescent method. The cholesterol concentration and the level of lipid peroxidation were significantly higher, whereas the concentration of proteins thiol groups decreased in the patient group. ATPase and GSH peroxidase activities diminished compared to those in the control group. There were no differences in either catalase or superoxide dismutase activities. The membrane fluidity was lower in erythrocytes from patients with MS than in the ones from control group. These results show changes in red blood cells of patients with MS as a consequence of a higher concentration of cholesterol in the membrane and an increased oxidative stress.

Role of membrane cholesterol and lipid peroxidation in regulating the Na+/K+-ATPase activity in schizophrenia

“…Although many studies reported a direct correlation between the membrane cholesterol and NKA activity,[12] some have observed an increased value of membrane cholesterol with decreased NKA activity, particularly in cases of metabolic syndrome. [13] Hence, significant lacunae still exist regarding the relationship between alterations in membrane cholesterol and NKA activity in different pathophysiological conditions including neuropsychiatric disorders.…”

New covalent modifications of phosphatidylethanolamine by alkanals: mass spectrometry based structural characterization and biological effects

“…[13][14][15][16][17][18] Specifically, peroxidation of membrane (phospho)lipids (PLs) can impair membrane functions, decrease fluidity, lead to inactivation of membrane-bound receptors and increase permeability to ions and even cause membrane rupture. [19] High levels of oxidative stress can even induce cell death directly by necrosis, while mild oxidative stress can trigger the process of apoptosis. [20] Lipid peroxidation occurs when ROS react with the lipid-esterified fatty acyl residues, particularly with polyunsaturated ones.…”

In vitro effects of prolonged exposure to quercetin and epigallocatechin gallate of the peripheral blood mononuclear cell membrane

“…Ziobro et al observed that the concentration of thiol groups in erythrocyte membranes from patients with metabolic syndrome was significantly lower (about 16%) compared to the control group. The results were correlated with a substantially lower membrane fluidity (metabolic syndrome vs. control) [44]. The biochemical profile of the investigated patients indicated the increase in the CVD risk for HC patients compared to NC subjects.…”