Oxidative damage of 18S and 5S ribosomal RNA in digestive gland of mussels exposed to trace metals

Kournoutou, Georgia G.; Giannopoulou, Panagiota; Sazakli, Eleni; Leotsinidis, Michalis; Kalpaxis, Dimitrios L.

doi:10.1016/j.aquatox.2017.09.007

Cited by 15 publications

(15 citation statements)

References 92 publications

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“…Trace metals are serious stressors in aquatic ecosystems that can interfere with various physiological processes in the marine organism mussel Mytilus galloprovincialis. In a previous study we evaluated the impact of these metals on the RNA modifications of the protein synthesis machinery Preprints (www.preprints.org) | NOT PEER-REVIEWED | Posted: 26 September 2020 doi:10.20944/preprints202009.0642.v1 [10]. In this project we explored the damage in the same machinery under the same exposure conditions from the viewpoint of protein expression and modification.…”

Section: Discussionmentioning

confidence: 99%

“…The pellet including ribosomes and polysomes was treated with puromycin (0.5 mM) in the presence of 0.5 M ammonium chloride [15]. After a centrifugation at 100,000×g for 7.5 h at 4 °C, the pellet was used for ribosomal preparation [10], while the supernatant was concentrated with ammonium sulphate treatment and used as fraction enriched in translation factors, named Washed Ribosomal Factors (FWR) fraction.…”

Section: B Fwr Protein Fractionmentioning

confidence: 99%

“…However, large concentrations of Cu become harmful [3,4], because of its propensity to mediate the formation of reactive oxygen species (ROS) [4,5]. In addition, Cu reacts with thiol groups, and thus is able to bind cysteine and inactivates proteins [6], induces the expression of metallothionein (MT) genes [7,8], cause lysosomal membrane destabilization [9,10,11,12] and perturbs the nucleus integrity [10,11,12,13,14,15]. Similarly, Cd inactivates many functional proteins by inducing widespread misfolding and aggregation through binding to S, N and O protein metal concentration was maintained.…”

Section: Introductionmentioning

confidence: 99%

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Oxidative Damage of Mussels Living in Seawater Enriched with Trace Metals, from the Viewpoint of Proteins Expression and Modification

Kournoutou¹,

Giannopoulou²,

Sazakli³

et al. 2020

Preprint

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The impact of metals bioaccumulation on marine organisms is under investigation. This study was designed to determine the association of oxidative stress in mussels Mytilus galloprovincialis induced by seawater enriched with trace metals with protein synthesis. Mussels were exposed to 40 μg/L Cu, 30 μg/L Hg, or 100 μg/L Cd for 5 and 15 days, and the pollution effect was evaluated by measuring established oxidative biomarkers. The results showed damage on the protein synthesis machine integrity and specifically, on translation factors and ribosomal proteins expression and modifications. Exposure of mussels to all metals caused oxidative damage that was milder in the cases of Cu and Hg, and more pronounced for Cd. However, after prolonged exposure of mussels to Cd (15 days), the effects receded. These changes that perturb protein biosynthesis can serve as a great tool for elucidating the mechanisms of toxicity and could be integrated in biomonitoring programs.

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Section: Discussionmentioning

confidence: 99%

Section: B Fwr Protein Fractionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Oxidative Damage of Mussels Living in Seawater Enriched with Trace Metals, from the Viewpoint of Proteins Expression and Modification

Kournoutou¹,

Giannopoulou²,

Sazakli³

et al. 2020

Preprint

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show abstract

“…However, large concentrations of Cu become harmful [3,4] because of its propensity to mediate the formation of reactive oxygen species (ROS) [4,5]. In addition, Cu reacts with thiol groups; thus, it is able to bind cysteine and inactivates proteins [6], induces the expression of metallothionein (MT) genes [7,8], causes lysosomal membrane destabilization [9][10][11][12], and perturbs the nucleus integrity [10][11][12][13][14][15]. Similarly, Cd inactivates many functional proteins by inducing widespread misfolding and aggregation through binding to S, N, and O protein atoms [16], destabilizes lysosomal membranes [10], and induces the expression of MT genes [8].…”

Section: Introductionmentioning

confidence: 99%

“…Alterations in gene expression under oxidative stress have been extensively analyzed in mollusks through RNA profiling techniques, microarrays, and RNA sequencing [28][29][30][31][32].Moreover, translational regulation generally contributes to quick responses related to the maintenance of proteome homeostasis, in contrast to transcriptional regulation that is mostly associated with long-term changes in cell physiology [33][34][35]. It has been also recognized that the molecular mechanisms underlying the metal toxicity on translation are associated with the vulnerability of the ribosomal components to oxidative damage [10,[36][37][38][39]. Proteins can scavenge up to 75% of oxidative insults, with ribosomal proteins being the most likely class of proteins to be oxidized [39,40].…”

Section: Introductionmentioning

confidence: 99%

Oxidative Damage of Mussels Living in Seawater Enriched with Trace Metals, from the Viewpoint of Proteins Expression and Modification

Kournouto

Giannopoulou

Sazakli

et al. 2020

Toxics

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The impact of metals bioaccumulation in marine organisms is a subject of intense investigation. This study was designed to determine the association between oxidative stress induced by seawater enriched with trace metals and protein synthesis using as a model the mussels Mytilus galloprovincialis. Mussels were exposed to 40 μg/L Cu, 30 μg/L Hg, or 100 μg/L Cd for 5 and 15 days, and the pollution effect was evaluated by measuring established oxidative biomarkers. The results showed damage on the protein synthesis machine integrity and specifically on translation factors and ribosomal proteins expression and modifications. The exposure of mussels to all metals caused oxidative damage that was milder in the cases of Cu and Hg and more pronounced for Cd. However, after prolonged exposure of mussels to Cd (15 days), the effects receded. These changes that perturb protein biosynthesis can serve as a great tool for elucidating the mechanisms of toxicity and could be integrated in biomonitoring programs.

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Effects of 4‐Br‐A23187 on Bacillus subtilis cells and unilamellar vesicles reveal it to be a potent copper ionophore

et al. 2022

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Ionophores are small molecules or peptides that transport metal ions across biological membranes. Their transport capabilities are typically characterized in vitro using vesicles and single ion species. It is difficult to infer from these data which effects ionophores have on living cells in a complex environment (e.g., culture medium), since net ion movement is influenced by many factors including ion composition of the medium, concentration gradients, pH gradient, and protein-mediated transport processes across the membrane. To gain insights into the antibacterial mechanism of action of the semisynthetic polyether ionophore 4-Br-A23187, known to efficiently transport zinc and manganese in vitro, we investigated its effects on the gram-positive model organism Bacillus subtilis. In addition to monitoring cellular ion concentrations, the physiological impact of treatment was assessed on the proteome level. 4-Br-A23187 treatment resulted in an increase in intracellular copper levels, the extent of which depended on the copper concentration of the medium. Effects of copper accumulation mirrored by the proteomic response included oxidative stress, disturbance of proteostasis, metal and sulfur homeostasis. The antibiotic effect of 4-Br-A23187 is further aggravated by a decrease in intracellular manganese and magnesium. A liposome model confirmed that 4-Br-A23187 acts as copper ionophore in vitro.

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Oxidative damage of 18S and 5S ribosomal RNA in digestive gland of mussels exposed to trace metals

Cited by 15 publications

References 92 publications

Oxidative Damage of Mussels Living in Seawater Enriched with Trace Metals, from the Viewpoint of Proteins Expression and Modification

Oxidative Damage of Mussels Living in Seawater Enriched with Trace Metals, from the Viewpoint of Proteins Expression and Modification

Oxidative Damage of Mussels Living in Seawater Enriched with Trace Metals, from the Viewpoint of Proteins Expression and Modification

Effects of 4‐Br‐A23187 on Bacillus subtilis cells and unilamellar vesicles reveal it to be a potent copper ionophore

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