Oxidative damage in trout erythrocyte in response to “in vitro” copper exposure

Fedeli, Donatella; Carloni, Manuel; Falcioni, Giancarlo

doi:10.1016/j.marenvres.2009.10.001

Cited by 20 publications

(16 citation statements)

References 19 publications

(8 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In our study, an increase of TAC in plasma of fish fed the diet supplemented with PCE diet was observed. We analyzed the expression of genes coding for antioxidant enzymes in fish erythrocytes, as they have been previously used to monitor cellular oxidative stress response toward environmental and nutritional cues (Gwoździński et al ; Roche and Boge ; Fedeli et al , , Trenzado et al ). Erythrocytes transport oxygen in blood via a reversible association to hemoglobin, and as a result they are exposed to ROS and consequently to oxidative stress (Saltman ).…”

Section: Discussionmentioning

confidence: 99%

Dietary Intake of Purple Corn Extract Reduces Fat Body Content and Improves Antioxidant Capacity and n‐3 Polyunsaturated Fatty Acid Profile in Plasma of Rainbow Trout, Oncorhynchus mykiss

Villasante

Patro

Chew

et al. 2015

J World Aquaculture Soc

View full text Add to dashboard Cite

A study was conducted to determine the benefits of the intake of purple corn extract (PCE), a natural source of anthocyanins, in rainbow trout, Oncorhynchus mykiss. A control or test diet supplemented with 5% PCE was fed to triplicate tanks with 25 fish each for 8 wk. The concentrations of three anthocyanins, cyanidin-3-glucoside, pelargonidin-3-glucoside, and peonidin-3-glucoside, were measured in PCE. The chemical composition and fatty acid profiles of fish, as well as the fatty acid profiles in plasma, liver, and muscle were determined. The total antioxidant capacity (TAC) and the concentration of oxidative damage biomarkers, for example, protein carbonyls, 8-hydroxydeoxyguanosine, and thiobarbituric acid reactive substances (TBARS), were determined in plasma. Transcription of two antioxidant enzymes, glutathione peroxidase 1 (gpx1) and superoxide dismutase 1 (sod1), was measured in erythrocytes. Significant lower adiposity and significant higher percentage of total n-3 and total n-6 polyunsaturated fatty acids (PUFA) in the body of fish fed the test diet were detected. Significant higher plasma percentage of total n-3 PUFA, significant higher plasma TAC, and significant higher expression of gpx1 in erythrocytes of fish fed the test diet were measured. Overall, our results suggest potential protection against in vivo lipid peroxidation in fish fed the PCE supplemented diet. This is especially true due to detection of an enhanced antioxidant protection in plasma and erythrocytes in fish, the reduced adiposity and greater proportion of total n-3 and n-6 PUFA in the fish body, as well as a tendency toward lower TBARS plasma concentration in fish fed the test diet when compared to the control group.

show abstract

Section: Discussionmentioning

confidence: 99%

Dietary Intake of Purple Corn Extract Reduces Fat Body Content and Improves Antioxidant Capacity and n‐3 Polyunsaturated Fatty Acid Profile in Plasma of Rainbow Trout, Oncorhynchus mykiss

Villasante

Patro

Chew

et al. 2015

J World Aquaculture Soc

View full text Add to dashboard Cite

show abstract

“…TBARS value increased as a result of poor antioxidative capability. Fedeli et al () and Tang et al () reported that an excess of copper may increase the formation of ROS leading to damage of proteins, lipids and DNA. Higher values of TBARS were noted in H. fossilis fed diets containing lower and higher levels of copper than those who were fed optimum dietary copper, indicating that oxidative stress was induced in fish fed both copper‐deficient diet as well as diet having excess levels of copper.…”

Section: Discussionmentioning

confidence: 99%

“…Surfeit amount of copper can also lead to damage of cells and organs such as severe lesions on the intestine, liver, brain and gills (Berntssen, Hylland, Wendelaar Bonga, & Maage, 1999;Clearwater et al, 2002). Damage of proteins, lipids and DNA by reactive oxygen species is activated by copper ions and its complexes (Damasceno et al, 2016;Fedeli, Carloni, & Falcioni, 2010;Tang et al, 2017). Copper is also a vital component of the ceruloplasmin enzyme and plays important role in iron metabolism.…”

mentioning

confidence: 99%

Dietary copper requirement of fingerlingHeteropneustes fossilisfor formulating copper‐balanced commercial feeds

Zafar

Khan

2019

Aquacult Nutr

View full text Add to dashboard Cite

Dietary copper requirement of Heteropneustes fossilis (6.74 ± 0.03 g) was determined by feeding purified diets containing same protein (400 g/kg) and gross energy (17.89 kJ/g) but different levels of copper for 12 weeks. Graded amount of CuSO4.5H2O (0, 1.96, 3.93, 5.89, 7.86, 9.82, 11.79 mg/kg) was supplemented to basal diet to attain desired dietary copper levels (0, 0.5, 1.0, 1.5, 2.0, 2.5 and 3.0 mg/kg). Analysed dietary copper concentrations were 4.28, 4.63, 5.28, 5.70, 6.19 and 6.69 mg/kg. Absolute weight gain, feed conversion ratio and protein gain improved with the increasing levels of dietary copper up to 5.28 mg/kg. Further inclusion of copper at a level of 5.70 mg/kg did not improve the above parameters. Significantly higher (p < .05) plasma ceruloplasmin, liver copper‐zinc superoxide dismutase, catalase activities and lower thiobarbituric acid reactive substances were evident in fish receiving diets with 5.28 and 5.70 mg/kg copper compared to other groups. Whole body and liver copper concentrations increased significantly (p < .05) with increasing dietary copper levels. Quadratic regression analysis of absolute weight gain, feed conversion ratio, protein gain and broken‐line regression analysis of plasma ceruloplasmin activity and liver TBARS value against the variable dietary copper levels depicted the dietary copper requirements for fingerling H. fossilis in the range of 5.24–5.68 mg/kg.

show abstract

“…Enhanced erythrocyte MNi frequencies were also observed in Channa punctata exposed to 0.4 mg/L copper for 24 to 168 h (Yadav and Trivedi 2009) and in the estuarine guppy Poecilia vivipara after a 96-h exposure to 9 mg/L Cu (Machado et al 2013). Finally, in vitro studies showed that incubation of rainbow trout (O. mykiss) gill homogenates with cooper resulted in altered DNA integrity by inducing single-and doublestranded DNA breaks (Arabi and Alaeddini 2005), though DNA damage was not observed in erythrocyte of this same species in response to copper exposure (Fedeli et al 2010). Taken altogether, these findings indicate that as related to lethality and the induction of MN, there is a differential sensitivity to Cu by different fish species and also a differential response to different metals in each fish species, with D. labrax being highly sensitive.…”

Section: Discussionmentioning

confidence: 99%

Early genotoxic response and accumulation induced by waterborne copper, lead, and arsenic in European seabass, Dicentrarchus labrax

Canalejo

Díaz-de-Alba

Granado-Castro

et al. 2015

Environ Sci Pollut Res

View full text Add to dashboard Cite

Cu, Pb, and As, which are among the most abundant metals in the aquatic environment, are also among the most health-threatened by causing diverse cellular injuries. The aim of this study was to assess and compare the potential early induction of genotoxic effects after waterborne Cu, Pb, and As exposure in European seabass, Dicentrarchus labrax, a commercial widely cultured fish, using the micronucleus (MN) assay in peripheral blood erythrocytes. Fish were exposed under laboratory conditions to nominal solutions ranging 0-10 mg/L for 24 and 96 h. Furthermore, actual metal ion concentrations were measured by inductively coupled plasma atomic emission spectroscopy (ICP-AES) or differential pulse anodic stripping voltammetry (DPASV) in water and four fish tissues differentially related to environmental exposition and metal accumulation, i.e. the gills, liver, muscle, and brain. Dose-dependent increases of micronuclei (MNi) frequency were observed after these very short exposures; based on measured metal concentrations in water, the genotoxic effect ordered as Cu > As > Pb. Significant genotoxic effect at 0.009 mg/L Cu, 0.57 mg/L Pb, and 0.01 mg/L As was seen. For Cu and Pb these are only slightly higher, but for As it is notably lower than the USEPA criteria of maximum concentration to prevent acute toxicity in aquatic organisms.Furthermore, genotoxicity was differentially related to metal accumulation. MNi frequency correlated positively with the content of Pb in all the organs, with the content of As in liver and gills and only with the content of Cu in the brain. In conclusion, our findings raised environmental concerns because these depicted a genotoxic potential of Cu, Pb, and As after a very short exposure to low but environmentally relevant concentrations, too close to regulatory thresholds. In addition, the MN test in D. labrax could be considered an early biomarker of genotoxicity induced by these metals in fish.

show abstract

Oxidative damage in trout erythrocyte in response to “in vitro” copper exposure

Abstract: 1The oxidative action of copper on different trout Oncorhynchus mykiss erythrocyte components was 2 studied. The results indicate that:

Cited by 20 publications

References 19 publications

Dietary Intake of Purple Corn Extract Reduces Fat Body Content and Improves Antioxidant Capacity and n‐3 Polyunsaturated Fatty Acid Profile in Plasma of Rainbow Trout, Oncorhynchus mykiss

Dietary Intake of Purple Corn Extract Reduces Fat Body Content and Improves Antioxidant Capacity and n‐3 Polyunsaturated Fatty Acid Profile in Plasma of Rainbow Trout, Oncorhynchus mykiss

Dietary copper requirement of fingerlingHeteropneustes fossilisfor formulating copper‐balanced commercial feeds

Early genotoxic response and accumulation induced by waterborne copper, lead, and arsenic in European seabass, Dicentrarchus labrax

Contact Info

Product

Resources

About