Oxidative Damage in the Aging Heart: an Experimental Rat Model

Marques, Gustavo Lenci; Neto, Francisco Filipak; Ribeiro, Ciro Alberto de Oliveira; Liebel, Samuel; Fraga, R.; Bueno, Ronaldo da Rocha Loures

doi:10.2174/1874192401509010078

Cited by 4 publications

(6 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Відповідно до мітохондріальної теорії старіння, основний фактор у розвитку вікових змін в органах і тканинах цілісного організмузміни функціонального стану мітохондрій, що викликає надмірне утворення активних форм оксигену (Judge and Leeuwenburgh, 2007;Marques et al, 2015;Anayt et al, 2017).…”

Section: вступunclassified

“…Каталазаодин із найважливіших ензимів антиоксидантної системи, який вже при мінімальних кількостях перекису гідрогену проявляє свою активність і таким чином захищає клітину від розвитку окисного стресу (Marni et al, 2013;Serova et al, 2016;Dyomshina et al, 2017). Співавтори (Marques et al, 2015) розглядають каталазу разом із супероксиддисмутазою та непротеїновими тіолами цитоплазми як єдине окисно-відновне середовище клітини. У своїй праці автори стверджують, що активність каталази зменшується у щурів віком 24 місяці на тлі збільшення концентрації непротеїнових тіолів (головним чином представлених глутатіоном).…”

Section: вступunclassified

“…З огляду на це, встановлений рівень ТБК-активних продуктів означає, що процеси ПОЛ і руйнування протеїнів клітинних мембран уже відбулися, і система антиоксидантного захисту намагається пристосуватися до таких навантажень для забезпечення енантіостазу. Це підтверджується літературними даними (Judge and Leeuwenburgh, 2007;Marques et al, 2015;Anayt et al, 2017).…”

Section: обговоренняunclassified

See 2 more Smart Citations

The role of mitochondria in the myocardium of senescent Meriones unguiculates

Potapenko¹,

Dyomshina²,

Ушакова³

2017

Regul. Mech. Biosyst.

View full text Add to dashboard Cite

According to the mitochondrial theory of aging, changes in the functional state of mitochondria, which lead to excessive formation of active forms of oxygen, are the main factor in the development of age changes in organs and tissues of the whole organism. The assessment of the mitochondria state of the heart of senescent gerbils (Mongolian Gerbilia, Meriones unguiculates Milne-Edwards, 1867). It is proved that the aging of the heart is preceded by the appearance of dysfunction in mitochondria. The disturbance of metabolic processes in the myocardium of gerbils over the age of two years was established, which was accompanied by activation of oxidative stress by increasing the concentration of TBK-active compounds. An increase in the concentration of cytochrome C in cytosol has been shown due to the destructive effects of oxidized products on the outer membrane of mitochondria and enhancement of its permeability. The violation of bioenergetic processes, increase of the anaerobic respiration and the accumulation of lactate and unoxidative metabolites, which increases oxidative stress and cell damage, are determined. We established that for gerbils the critical age for senescence is 24 months. Major metabolic changes in the heart occur mostly at this age. This is marked by activation of prooxidants formation, proteolytic processes (decrease in total protein concentration) and inhibition of aminotransferase activity in cytosol. The switching of metabolic processes in the mitochondria of the heart with the participation of aminotransferases has been registered: increased activity of the mitochondrial isoenzyme alanine aminotransferase and reciprocal reduction of aspartate aminotransferase. After two years of age in the experimental gerbils the intensification of adaptive processes was established: activation of catalase, γ-glutamyltransferase, relative restoration of the activity of alanine and aspartate aminotransferase, thus maintaining the processes of the vital activity of the whole organism, but at a new metabolic level. With age, irreversible damage to cardiomyocytes occurs, which gradually lose the ability to convert lactate, resulting in its increase, and the processes of its utilization are inhibited. The results confirm the key role of mitochondria in the process of aging of the myocardium. However, when gerbils reach the 36 months of age the metabolic disturbances in the myocardium reach their peak, resulting in large-scale cell damage. Роль мітохондрій міокарда піщанок упродовж фізіологічного старінняЄ. О. Потапенко, О. О. Дьомшина, Г. О. Ушакова Дніпровський національний університет імені Олеся Гончара, Дніпро, УкраїнаНаведено дані щодо оцінки мітохондрій та біохімічного стану серця монгольських піщанок (Mongolian Gerbilia, Meriones unguiculatus Milne-Edwards, 1867) в умовах старіння. Основний фактор розвитку вікових змін в органах і тканинах цілісного організмунадмірне утворення активних форм оксигену, що провокує зміни функціонального стану мітохондрій, зниження ефективності процесу аутофагії / мітофаг...

show abstract

Section: вступunclassified

Section: обговоренняunclassified

See 1 more Smart Citation

The role of mitochondria in the myocardium of senescent Meriones unguiculates

Potapenko¹,

Dyomshina²,

Ушакова³

2017

Regul. Mech. Biosyst.

View full text Add to dashboard Cite

show abstract

“…Consequently, due to the absence of fish death, LD50 was not able to estimate when the maximum dose of diclazuril, but according to the results of the study, it is greater than 15 g/kg weight. In accordance with the classification proposed by Medved' , Kagan and Spynu (1968) and adopted by the WHO, the toxicity of the test drug can be classified as non-expressed, and regarding the state standard (GOST 12.1.007-76) diclazuril can be classified as toxicity class IV.…”

mentioning

confidence: 97%

“…It could be explained by occurring of compensatory expenditure of a pool of endogenous structural antioxidants (at TAC decreasing) and adaptogenic enhancement of catalase activity: the maximum expressed changes in the values of the parameters were found on the 28 th and 14 th day of the experiment. It is known that catalase plays a major role in the adaptation of cells to the increased intensity of catabolic and destruction (Marques et al, 2015), and the determined dynamics of this enzyme as a result of the introduction of the drug is cells adaptation to maintain the processes of lipoperoxidation in their membranes at the physiological level.…”

mentioning

confidence: 99%

Acute and sub-acute toxicity assessment of ‘Rybozuril’ on common carp

Yevtushenko

Boyko

Romanko

2019

J. Vet. Med. Biotechnol. Biosafety

View full text Add to dashboard Cite

The goal of the work was to study of acute and sub-acute toxicity parameters of ‘Rybozuril’ biological product (AI — diclazuril) on the model of carp. This drug is effective in the treatment of diseases caused by parasitic Eimeriidae. Carp scales of two years old were used in experiments. To determine acute toxicity, the fish were prescribed with diclazuril in doses of 1 g/kg, 5, 10, and 15 g/kg of live weight. Two experimental and control fish groups of 30 individuals each were formed to determine sub-acute toxicity of ‘Rybozuril’. Experimental groups of fish were prescribed with ‘Rybozuril’ in a dose (by AI) of 50 and 10 mg/kg for two consecutive days. Blood samples were collected from six fish from each group for clinical and biochemical indicators after 2, 7, 14, 21, and 28 days. The hemoglobin content, number of red blood cells and leukocytes blood was determined. The intensity of peroxide oxidation of lipids (PОL), catalase activity, level of total antioxidant capacity (TAC), total proteins, albumin, globulins and glucose, circulating immune complexes (CІC) and seromucoids concentration, level of enzymatic activity: aspartate transaminase (АSТ), alanine transaminase (АLТ), α-amylase blood plasma were determined. The acute toxicity of diclazuril for carp was estimated, LD50 is more than 15,000 mg/kg of live weight, the toxicity of diclazuril can be classified as undifferentiated and, in terms of toxicity, it can be classified as hazard class IV. Two administrations of the drug ‘Rybozuril’ in a daily dose of 50.0 mg/kg of live weight, the maximum expression of metabolic changes in fish was detected from the initial terms of the studies and up to day 21. According to the results the toxic effect of the drug in fish was estimated, which did not influence to a number of indicators. The drug in such dose was shown immunosuppression and membrane-toxic effects in fish. Two-time administration of the ‘Rybozuril’ drug in a daily dose of 10.0 mg/kg body weight leads to metabolic alterations in fish due to the activation of detoxification processes and lipoperoxidation maintenance in cell membranes at the physiological level. At the end of the experiment the toxic effect of ‘Rybozuril’ in fish characterized by stable parameters in comparing to the control group.

show abstract