Oxidative damage and antioxidant response of <i>Allium cepa</i> meristematic and elongation cells exposed to metronidazole

Andrioli, Nancy Beatriz; Sabatini, Sebastián E.; Mudry, Marta Dolores; Molina, Marı́a del Carmen

doi:10.1002/etc.1799

Cited by 4 publications

(1 citation statement)

References 39 publications

(44 reference statements)

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“…In vivo experiment without neutrophils, neither dose-dependence nor antioxidant effects were observed [28]. Andrioli et al studied the toxicity of metronidazole on the roots of Allium cepa for 30 h. They found a significant increase in the antioxidant defence parameters attributed to metronidazole-induced oxidative stress [45]. Metronidazole applied against Trichomoniasis hydrogen peroxide, superoxide and nitrogen-free radicals were activated [20,21].…”

Section: Discussionmentioning

confidence: 99%

Metronidazole does not show direct antioxidant activity in in vitro global systems

Szentmihályi

Süle

Egresi

et al. 2021

Heliyon

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Metronidazole has been widely used topically and systemically for more than 50 years but data on its antioxidant properties are still incomplete, unclear and contradictory. Its antioxidant properties are primarily hypothesized based on in vivo results, therefore, studies have been performed to determine whether metronidazole has antioxidant activity in vitro. We used so-called global spectrophotometric and luminometric methods. Fe 3þ /Fe 2þreducing ability, hydrogen donor activity, hydroxyl radical scavenging property and lipid peroxidation inhibitory activity were investigated. Under the condition used, metronidazole has negligible iron-reducing ability and hydrogen donor activity. The hydroxyl radical scavenging capacity cannot be demonstrated. It acts as a prooxidant in the H 2 O 2 / . OH-microperoxidase-luminol system, but it can inhibit the induced lipid peroxidation. According to our results, metronidazole has not shown antioxidant activity in vitro but can affect redox homeostasis by a ROS-independent mechanism due to its non-direct antioxidant properties.

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Section: Discussionmentioning

confidence: 99%

Metronidazole does not show direct antioxidant activity in in vitro global systems

Szentmihályi

Süle

Egresi

et al. 2021

Heliyon

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Detrimental Effects of Metronidazole on the Liver of Freshwater Common Carp (Cyprinus carpio L.)

Han

Cai

Wang

et al. 2013

Bull Environ Contam Toxicol

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The widely used antibiotic metronidazole (MTZ) was investigated for its toxic effects on the liver of the common carp (Cyprinus carpio L.). The fish were exposed to 0.1, 0.5, and 2.5 mg/L MTZ in water for 30 days, and parameters that are indicative of liver damage and oxidative stress were measured. MTZ increased liver ethoxyresorufin-O-deethylase, malondialdehyde, and glutathione levels, and elevated the activities of superoxide dismutase, catalase, aspartate aminotransferase, and alanine aminotransferase. These parameters usually showed significant differences in the 0.5 and 2.5 mg/L MTZ groups compared to the control group (p < 0.05). These findings indicated that MTZ induced oxidative stress and caused liver damage in common carp, suggesting that measures should be taken to avoid contamination of surface waters with MTZ.

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Glucose-6-phosphate dehydrogenase plays a pivotal role in tolerance to drought stress in soybean roots

Liu

Wang

et al. 2012

Plant Cell Rep

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KEY MESSAGE : Two soybean cultivars showed markedly different drought tolerance. G6PDH plays a central role in the process of H ( 2 ) O ( 2 ) regulated GR, DHAR, and MDHAR activities to maintain GSH and Asc levels. Glucose-6-phosphate dehydrogenase (G6PDH) plays a pivotal role in plant resistance to environmental stresses. In this study, we investigated the role of G6PDH in modulating redox homeostasis under drought stress induced by polyethylene glycol 6000 (PEG6000) in two soybean cultivars JINDOU21 (JD-21) and WDD00172 (WDD-172). The G6PDH activity markedly increased and reached a maximum at 96 h in JD-21 and 72 h in WDD-172 during PEG6000 treatments, respectively. Glucosamine (Glucm, a G6PDH inhibitor) obviously inhibited G6PDH activity in both soybeans under PEG6000 treatments. After PEG6000 treatment, JD-21 showed higher tolerance than WDD-172 not only in higher activities of superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), glutathione reductase (GR), dehydroascorbate reductase (DHAR), and monodehydroascorbate reductase (MDHAR), but also in higher content of glutathione (GSH) and ascorbate (Asc). And we found that hydrogen peroxide (H(2)O(2)) regulated the cell length in root elongation zone. Diphenylene iodonium (DPI, a plasma membrane NADPH oxidase inhibitor) counteracted the PEG6000-induced H(2)O(2) accumulation and decreased the activities of GR, DHAR, and MDHAR as well as GSH and Asc content. Furthermore, exogenous application of H(2)O(2) increased the GR, DHAR, and MDHAR activities that were decreased by Glucm under drought stress. Western blot analysis showed that the G6PDH expression was stimulated by PEG6000 and buthionine sulfoximine (BSO, glutathione biosynthesis inhibitor), and blocked by Glucm, DPI and N-acetyl-L-cysteine (NAC, GSH precursor) in both cultivars. Taken together, our evidence indicates that G6PDH plays a central role in the process of H(2)O(2) regulated GR, DHAR, and MDHAR activities to maintain GSH and Asc levels.

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Oxidative damage and antioxidant response of Allium cepa meristematic and elongation cells exposed to metronidazole

Cited by 4 publications

References 39 publications

Metronidazole does not show direct antioxidant activity in in vitro global systems

Metronidazole does not show direct antioxidant activity in in vitro global systems

Detrimental Effects of Metronidazole on the Liver of Freshwater Common Carp (Cyprinus carpio L.)

Glucose-6-phosphate dehydrogenase plays a pivotal role in tolerance to drought stress in soybean roots

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