Oxidation potentials of guanine, guanosine and guanosine-5′-monophosphate: Theory and experiment

Liška, Alan; Třísková, Iveta; Ludvı́k, Jiřı́; Trnková, Libuše

doi:10.1016/j.electacta.2019.06.052

Cited by 13 publications

(11 citation statements)

References 61 publications

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“…5, the oxidation peak current of [Ru(bpy) 2 dpp] 2+ →[Ru(bpy) 2 dpp] 3+ + e − remained constant with pH. On the contrary, the oxidation peak current of 5ʹ-GMP decreased significantly with increasing pH, which was consistent with studies related to the greater susceptibility of 5ʹ-GMP to oxidation under acidic conditions [31]. The oxidation peak current of 5ʹ-GMP was maximum at pH 3, which is consistent with the choline monolayer carrier and gold nanocavity functionalized carbon nanotube sensing interface (pH 4) and carboxylated multi-walled carbon nanotubes/AuNPs modified glassy carbon electrode (pH 3) in comparison [32,33].…”

Section: Optimization Experiments Optimization Analysis Of the Ph Of ...supporting

confidence: 87%

An electrochemical sensor based on [Ru(bpy)2dpp]2+/SMWCNTs/Au modified glassy carbon electrode for the detection of 5ʹ-GMP

Huan

Wang

et al. 2022

Appl Biol Chem

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A sensitive electrochemical sensor for the selective detection of 5ʹ-guanylic acid (5ʹ-GMP) was prepared by combining sulfonated-multiwalled carbon nanotubes (SMWCNTs) and [Ru(bpy)2dpp]Cl2, which were dripped on the surface of a glass carbon electrode (GCE) immobilized with gold nanoparticles. The 5ʹ-GMP electrochemical biosensor was fabricated using [Ru(bpy)2dpp]2+/SMWCNTs/Au/GCE as working, Ag/AgCl as reference and Pt as auxiliary electrode connected by an electrochemical workstation. The modified electrode was characterized by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The results showed the sensor’s response current had the best peak shape and maximum peak when the pH of electrolyte was 3, scan speed of CV was in the range of 100 to 180 mV/s, and the enrichment time was in the range of 200 to 300 s. Under the optimum conditions, a linear analytical curve was obtained for 5ʹ-GMP concentrations in the range of 0.01 to 0.5 mmol L−1, with a detection limit of 0.0014 mmol L−1. The analytical results of the 5ʹ-GMP sensor were exhibited good consistent with the data from liquid chromatography. The sensor has good reproducibility, long-term stability and strong immunity to interference, and may be a powerful device for 5ʹ-GMP detection, with great advantages such as simple preparation and operation, low equipment cost.

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Section: Optimization Experiments Optimization Analysis Of the Ph Of ...supporting

confidence: 87%

An electrochemical sensor based on [Ru(bpy)2dpp]2+/SMWCNTs/Au modified glassy carbon electrode for the detection of 5ʹ-GMP

Huan

Wang

et al. 2022

Appl Biol Chem

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“…The recorded CV curve showed the characteristic oxidation peak of MB at ∼−0.1/+0.1 V vs. the Ag/AgCl reference electrode, as shown in Figure 4a, as compared to the bare sensor. We additionally considered if the charge transfer arising from the oxidation/reduction of oligo bases, such as guanosine, 49 would interfere with the MB peak current. The response of two identical sensor strips functionalized with the same oligo sequence was tested with and without the MB functionality at its 3′ terminal.…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

Binding-Induced Folding of DNA Oligonucleotides Targeted to the Nucleocapsid Gene Enables Electrochemical Sensing of SARS-CoV-2

Alafeef

Skrodzki

Moitra

et al. 2023

ACS Appl. Bio Mater.

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In the wake of the COVID-19 pandemic, millions of confirmed cases and deaths have been reported around the world. COVID-19 spread can be slowed and eventually stopped by a rapid test to diagnose positive cases of the disease on the spot. It is still important to test for COVID-19 quickly regardless of the availability of the vaccine. Using the binding-induced folding principle, we developed an electrochemical test for detecting SARS-CoV-2 with no RNA extraction or nucleic acid amplification. The test showed high sensitivity with a limit of detection of 2.5 copies/μL. An electrode mounted with a capture probe and a portable potentiostat are used to conduct the test. To target the N-gene of SARS-CoV-2, a highly specific oligo-capturing probe was used. Based on the binding-induced “folding” principle, the sensor detects binding between the oligo and RNA. When the target is absent, the capture probe tends to form a hairpin as a secondary structure, retaining the redox reporter close to the surface. This can be seen as a large anodic and cathodic peak current. When the target RNA is present, the hairpin structure will open to hybridize with its complementary sequence, causing the redox reporter to pull away from the electrode. Consequently, the anodic/cathodic peak currents are reduced, indicating the presence of the SARS-CoV-2 genetic material. Validation of the test performance was performed using 122 COVID-19 clinical samples (55 positives and 67 negatives) and benchmarked to the gold standard reverse transcription-polymerase chain reaction (RT-PCR) test. As a result of our test, the accuracy, sensitivity, and specificity have been measured at 98.4%, 98.2%, and 98.5%, respectively.

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“…Guanosine, a purine nucleoside, is an important metabolite with a nucleobase guanine that is attached to an anomeric carbon of ribofuranose (a ribose) via a β‐N 9 ‐glycosidic bond. It is one of the important neuromodulators and neouroprotectors in the brain that reduces the effects of neurological events such as traumatic injuries and strokes . It triggers several important activities.…”

Section: Introductionmentioning

confidence: 99%

“…When it comes to guanosine monitoring and detection, there have been very few attempts using electrochemical techniques. Guanosine is an electroactive compound . For this reason, electrochemical approach can serve certain and significant benefits upon other techniques.…”

Section: Introductionmentioning

confidence: 99%

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Direct Electrochemistry and Sensitive Detection of Guanosine on Nanopolymeric Surfaces Bearing Boronic Acid Groups

Kuralay

Gürsoy

2020

ChemistrySelect

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This paper describes the use of novel boronic acid containing polymeric surfaces for following improved direct electrochemistry and highly sensitive and selective electrochemical detection of guanosine. Effective and robost boronic acid containing electroactive polymeric layers based on poly(3‐aminophenylboronic acid) (PAPBA) in the existence of a convenient nanomaterial (multi‐walled carbon nanotubes, MWCNTs) were fabricated onto graphite surfaces with a simple and reproducible single‐step electro‐fabrication method for the first time. So‐formed electrodes were then utilized for direct electrochemistry of guanosine and determination of guanosine. The electrode presented a linearity to guanosine from 1 μM to 120 μM and the detection limit was calculated as 0.14 μM (n=3). It was feasible to differentiate guanine and guanosine with a good peak separation value of 0.31 V. Furthermore, analytical performance of this novel electrode was compared with the performances of graphene added polymeric layer modified graphite electrode and unmodified graphite electrode.

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Oxidation potentials of guanine, guanosine and guanosine-5′-monophosphate: Theory and experiment

Cited by 13 publications

References 61 publications

An electrochemical sensor based on [Ru(bpy)2dpp]2+/SMWCNTs/Au modified glassy carbon electrode for the detection of 5ʹ-GMP

An electrochemical sensor based on [Ru(bpy)2dpp]2+/SMWCNTs/Au modified glassy carbon electrode for the detection of 5ʹ-GMP

Binding-Induced Folding of DNA Oligonucleotides Targeted to the Nucleocapsid Gene Enables Electrochemical Sensing of SARS-CoV-2

Direct Electrochemistry and Sensitive Detection of Guanosine on Nanopolymeric Surfaces Bearing Boronic Acid Groups

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