“…[ 33 , 34 ] Single‐stranded oligonucleotides (ssDNAs), which are fragments of nucleic acids, emerge as valuable tools in this process. [ 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 , 46 , 47 ] They can be precisely designed to exhibit high specificity and affinity for highly conserved plasmid and genomic regions of CT and NG. These ssDNAs that target the conserved regions play a critical role in achieving specific and sensitive detection of the pathogens, enabling accurate identification even in scenarios where target genetic concentrations are low and minimizing the occurrence of false positives.…”