Oxidation of plasma proteins is not increased after supplementation with eicosapentaenoic and docosahexaenoic acids

Wander, Rosemary C.; Du, Shi-Hua

doi:10.1093/ajcn/72.3.731

Cited by 75 publications

(56 citation statements)

References 36 publications

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“…Several human nutritional studies have indicated that supplementation with n 2 3 LCPUFA enhanced the marker of lipid peroxidation at dietary intakes of n 2 3 LCPUFA higher than those supplied in our study [31][32][33]. However, the influence of n 2 3 LCPUFA supplementation on the oxidative stress is still controversial.…”

Section: Discussionmentioning

confidence: 59%

Effect of docosahexaenoic acid and eicosapentaenoic acid supplementation on oxidative stress levels during pregnancy

Shoji¹,

Franke²,

Campoy³

et al. 2006

Free Radical Research

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Docosahexaenoic acid (DHA) is an indispensable component of cell membranes with high requirements during pregnancy. DHA supplementation is thought to enhance oxidative stress because of increased likelihood of lipid peroxidation. We estimated the oxidative stress levels in two groups of pregnant women who received daily supply of required vitamins with (n ¼ 23) or without (n ¼ 23) 500 mg of DHA and 150 mg of eicosapentaenoic acid (EPA) from 20 weeks of gestation to the time of delivery. Urinary excretions of 8-hydroxy-2 0 -deoxyguanosine (8-OHdG), a marker of oxidative DNA damage and of malondialdehyde (MDA), a marker of lipid peroxidation, were measured at 20, 30 weeks and at the time of delivery. Urinary MDA excretion remained unchanged throughout the study period in both groups. Urinary 8-OHdG excretion at delivery was significantly higher than at 20 and 30 weeks ( p , 0.05), but there were no group differences at the three time points. There were no differences between the two groups in plasma a-tocopherol levels. We conclude that under the conditions studied, a daily supplementation of 500 mg DHA and 150 mg EPA with vitamins to pregnant women did not enhance lipid peroxidation or oxidative DNA damage.

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Section: Discussionmentioning

confidence: 59%

Effect of docosahexaenoic acid and eicosapentaenoic acid supplementation on oxidative stress levels during pregnancy

Shoji¹,

Franke²,

Campoy³

et al. 2006

Free Radical Research

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“…Vários estudos têm relatado resultados contraditórios sobre o efeito do consumo de EPA e DHA na oxidação plasmática in vivo (14,34). Um estudo prévio demonstrou que a suplementação com esses áci-dos graxos aumentou discretamente a lipoperoxidação verificada pelo teste de TBARS, mas não a oxidação proteica.…”

Section: Tabelaunclassified

“…Os ácidos graxos de cadeia longa n-3, tais como o EPA e o DHA, oxidam mais prontamente que os outros ácidos graxos menos insaturados, tais como o ácido linoleico e, dessa forma, poderiam aumentar o estresse oxidativo (14).…”

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Efeito dos ácidos graxos n-3 no perfil glicêmico e lipídico, no estresse oxidativo e na capacidade antioxidante total de pacientes com síndrome metabólica

Simão

Godeny

Lozovoy

et al. 2010

Arq Bras Endocrinol Metab

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OBJETIVO: A síndrome metabólica (SM) é um conjunto de fatores que favorecem o desenvolvimento de doenças cardiovasculares. Estudos prévios demonstram que os ácidos graxos poliinsaturados (PUFAs) podem melhorar alguns desses fatores. O objetivo foi avaliar o efeito do óleo de peixe rico em PUFAs nos perfis glicêmico e lipídico, no estresse oxidativo e na capacidade antioxidante total (TRAP) no plasma em pacientes com SM. SUJEITOS E MÉTODOS: Foi realizado um ensaio clínico em 40 pacientes com SM (20 controles e 20 pacientes que consumiram 3 g/dia de PUFAs). RESULTADOS: O grupo que recebeu tratamento apresentou redução significativa nos níveis de triacilgliceróis e aumento no TRAP, mas sofreu aumento nos níveis de LDL, glicose e na resistência à insulina. CONCLUSÃO: Conclui-se que a ingestão de óleo de peixe foi capaz de diminuir os níveis de triacilgliceróis e aumentar o TRAP de pacientes com SM, porém verificou-se aumento nos níveis de LDL e na resistência à insulina.

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“…More importantly, in vivo, a large increase in tissue and plasma accumulation of both omega-3 fatty acids and fatty acid oxidation products is noted in subjects consuming fish oil, even after addition of antioxidant supplements to the diet. [7][8][9][10] This suggests the possibility that oxidized omega-3 fatty acids may be an important component of the observed antiinflammatory effects of fish oil. Indeed, our previous studies have shown that oxidized EPA, and not unoxidized EPA, potently inhibits leukocyte-endothelial interactions, both in vitro and in vivo, through a peroxisome proliferator-activated receptor (PPAR)␣-dependent mechanism.…”

mentioning

confidence: 99%

Oxidized Omega-3 Fatty Acids Inhibit NF-κB Activation Via a PPARα-Dependent Pathway

Mishra

Chaudhary

Sethi

2004

ATVB

168

109

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Objective-The aim of this study was to determine the effects of oxidized versus native omega-3 fatty acids on the endothelial expression of chemokines MCP-1 and IL-8, and, if effective in inhibiting chemokine expression, to determine the mechanism for the inhibition of chemokine expression. Methods and Results-Using enzyme-linked immunosorbent assays, we show that oxidized EPA and DHA but not unoxidized EPA or DHA inhibit cytokine-induced endothelial expression of monocyte chemoattractant protein (MCP)-1 and, to a lesser extent, IL-8. In electrophoretic mobility shift assays, oxidized EPA but not unoxidized EPA potently inhibited cytokine-induced activation of endothelial nuclear factor-B (NF-B). Using Western blot analyses, we show that the inhibition of NF-B activation was not caused by prevention of phosphorylation of I B␣ because oxidized EPA did not inhibit cytokine-induced phosphorylation and ubiquination of I B␣. Furthermore, oxidized EPA inhibited NF-B activation in endothelial cells derived from wild-type mice but had no inhibitory effects on NF-B activation in endothelial cells derived from peroxisome proliferator-activated receptor ␣ (PPAR␣)-deficient mice, indicating that oxidized EPA requires PPAR␣ for its inhibitory effects on NF-B. Conclusions-These studies show that the antiinflammatory effects of fish oil may result from the inhibitory effects of oxidized omega-3 fatty acids on NF-B activation via a PPAR␣-dependent pathway. (Arterioscler Thromb Vasc Biol. 2004;24:1621-1627.)Key Words: monocyte chemoattractant protein-1 Ⅲ oxidized omega-3 fatty acids Ⅲ oxidized eicosapentaenoic acid Ⅲ nuclear factor-B Ⅲ PPARa C onsumption of marine fish oil has been reported to improve the prognosis of several chronic inflammatory diseases characterized by leukocyte accumulation and leukocyte-mediated tissue injury, including atherosclerosis, IgA nephropathy, inflammatory bowel disease, rheumatoid arthritis, etc. [1][2][3][4] These beneficial effects of fish oil have been associated with the omega-3 polyunsaturated fatty acids (PUFAs), eicosapentaenoic acid (EPA), and docosahexaenoic acid (DHA), which are abundant in marine fish oil.EPA and DHA are highly polyunsaturated and easily undergo auto-oxidation. 5,6 In fact, it is very difficult to avoid the oxidation of these very labile fatty acids. More importantly, in vivo, a large increase in tissue and plasma accumulation of both omega-3 fatty acids and fatty acid oxidation products is noted in subjects consuming fish oil, even after addition of antioxidant supplements to the diet. [7][8][9][10] This suggests the possibility that oxidized omega-3 fatty acids may be an important component of the observed antiinflammatory effects of fish oil. Indeed, our previous studies have shown that oxidized EPA, and not unoxidized EPA, potently inhibits leukocyte-endothelial interactions, both in vitro and in vivo, through a peroxisome proliferator-activated receptor (PPAR)␣-dependent mechanism. 11,12 One of the early events in inflammation is the upregulation of endothelial ch...

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Oxidation of plasma proteins is not increased after supplementation with eicosapentaenoic and docosahexaenoic acids

Cited by 75 publications

References 36 publications

Effect of docosahexaenoic acid and eicosapentaenoic acid supplementation on oxidative stress levels during pregnancy

Effect of docosahexaenoic acid and eicosapentaenoic acid supplementation on oxidative stress levels during pregnancy

Efeito dos ácidos graxos n-3 no perfil glicêmico e lipídico, no estresse oxidativo e na capacidade antioxidante total de pacientes com síndrome metabólica

Oxidized Omega-3 Fatty Acids Inhibit NF-κB Activation Via a PPARα-Dependent Pathway

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