Effect of docosahexaenoic acid and eicosapentaenoic acid supplementation on oxidative stress levels during pregnancy

Shoji, Hiromichi; Franke, Corinna; Campoy, Cristina; Rivero, Monstserrat; Demmelmair, Hans; Koletzko, Berthold

doi:10.1080/10715760500539147

Cited by 23 publications

(12 citation statements)

References 38 publications

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“…Our results showing no increase in in vivo lipid peroxidation after DHA supplementation agree with those of several studies with fish oils or individual ( n -3) PUFAs in which either ex vivo LDL peroxidation or plasma or urinary isoprostane concentrations were measured. (12–20) Our results differ from those of studies showing an increase or decrease in ex vivo markers of oxidation after supplementation with ( n -3) PUFAs. (22–32) Our findings with DHA also differ from those of in vitro studies in which DHA increased lipid peroxidation when used at concentrations of 50–200 µmol/L, (40,42,43) but not at 25 µmol/L.…”

Section: Discussioncontrasting

confidence: 99%

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DHA concentration of red blood cells is inversely associated with markers of lipid peroxidation in men taking DHA supplement

Shichiri¹,

Adkins²,

Ishida³

et al. 2014

J. Clin. Biochem. Nutr.

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An increase in the proportion of fatty acids with higher numbers of double bonds is believed to increase lipid peroxidation, which augments the risk for many chronic diseases. (n-3) Polyunsaturated fatty acids provide various health benefits, but there is a concern that they might increase lipid peroxidation. We examined the effects of docosahexaenoic acid [22:6 (n-3)] supplementation on lipid peroxidation markers in plasma and red blood cells (RBC) and their associations with red blood cell and plasma fatty acids. Hypertriglyceridemic men (n = 17 per group) aged 39–66 years participated in a double-blind, randomized, placebo-controlled, parallel study. They received no supplements for the first 8 days and then received 7.5 g/day docosahexaenoic acid oil (3 g/day docosahexaenoic acid) or olive oil (placebo) for 90 days. Fasting blood samples were collected 0, 45, and 91 days after supplementation. Docosahexaenoic acid supplementation did not change plasma or RBC concentrations of lipid peroxidation markers (total hydroxyoctadecadienoic acid, total hydroxyeicosatetraenoic acid, total 8-isoprostaglandin F2α, 7α-hydroxycholesterol, 7β-hydroxycholesterol) when pre- and post-supplement values were compared. However, the post-supplement docosahexaenoic acid (DHA) concentration was inversely associated with RBC concentrations of ZE-HODE, EE-HODE, t-HODE, and total 8-isoprostaglandin F2α, (p<0.05). RBC concentration of hydroxycholesterol was also inversely associated with DHA but it did not attain significance (p = 0.07). Our results suggest that increased concentration of DHA in RBC lipids reduced lipid peroxidation. This may be another health benefit of DHA in addition to its many other health promoting effects.

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Section: Discussioncontrasting

confidence: 99%

“…Results from human studies with ( n -3) PUFAs regarding their effects on lipid peroxidation have been inconsistent; that is, no change, (12–21) increase, (22–28) or decrease in lipid peroxidation was observed. (29–32) In another study, the effect of DHA ranged from protection to increased lipid peroxidation depending on its dose.…”

Section: Introductionmentioning

confidence: 99%

DHA concentration of red blood cells is inversely associated with markers of lipid peroxidation in men taking DHA supplement

Shichiri¹,

Adkins²,

Ishida³

et al. 2014

J. Clin. Biochem. Nutr.

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“…Human studies indicate that dietary supplementation with n-3 fatty acids (fish oil) in pregnancy does not appear to increase the levels of markers of oxidative stress in maternal urine (Shoji et al 2006), but that it can reduce cord plasma F2-isoprostane levels in the infant ). This supplementation is associated with an increase in the length of gestation period and a reduced risk of preterm birth (Olsen et al 2000, Smuts et al 2003, Szajewska et al 2006, Horvath et al 2007, Makrides et al 2010, although the majority of studies have been limited by small sample sizes.…”

Section: Discussionmentioning

confidence: 99%

Differential effects of docosahexaenoic acid on preterm and term placental pro-oxidant/antioxidant balance

2013

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Docosahexaenoic acid (DHA) supplementation in pregnancy may confer some clinical benefits; however, this compound can exert pro-oxidant effects. In this study, we investigated the effects of DHA on pro-oxidant/antioxidant balance in term and preterm placental explants, assessing oxidative stress marker concentrations, antioxidant capacity and pro-inflammatory cytokine production. Term (nZ8) and preterm (nZ9) placental explants were exposed to lipopolysaccharide (LPS, 1 ng/ml), DHA (1, 10 and 100 mM), and DHA and LPS simultaneously or pre-treated with DHA for 24 h prior to LPS treatment. The production of malondialdehyde (MDA, lipid peroxidation), 8-hydroxy-2-deoxy guanosine (8-OHdG, oxidative DNA damage) and pro-inflammatory cytokines (tumour necrosis factor a (TNFa), interleukin 6 and interferon-g) and total antioxidant capacity were measured. DHA at a concentration of 100 mM induced oxidative stress in term placentas, while at all the three concentrations, it induced oxidative stress in preterm placentas. DHA and LPS resulted in reduced MDA levels in term (P!0.005) and preterm (PZ0.004) placentas and reduced 8-OHdG levels in preterm placentas (PZ0.035). DHA pre-treatment, but not co-treatment with LPS, reduced 8-OHdG levels (P!0.001) in term placentas. DHA increased antioxidant capacity only in term placentas (P!0.001), with lower antioxidant capacity being observed overall in preterm placentas compared with term placentas (P%0.001). In term placentas, but not in preterm ones, DHA co-treatment and pre-treatment reduced LPS-induced TNFa levels. The ability of DHA to alter placental pro-oxidant/antioxidant balance is dependent on the DHA concentration used and the gestational age of the placental tissue. DHA has a greater capacity to increase oxidative stress in preterm placentas, but it offers greater protection against inflammation-induced oxidative stress in term placentas. This appears to be a result of DHA altering placental antioxidant capacity. These data have implications for the timing and concentration of DHA supplementation in pregnancy.

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“…A recent randomized control trial [14] investigated markers of oxidative stress in pregnant women who received a daily supply of vitamins (including 3 mg of vitamin E and 30 mg of vitamin C) and either 500 mg of DHA and 150 mg of EPA from modified fish oil (n ¼ 23) or placebo (n ¼ 23) from 20 weeks of gestation until delivery. Urinary excretions of 8-hydroxy-2 0 -deoxyguanosine (8-OHdG), a marker of oxidative DNA damage, and of MDA, a marker of lipid peroxidation, were measured at 20 and 30 weeks of gestation as well as at the time of delivery.…”

Section: Oxidative Stress In Pregnancymentioning

confidence: 99%

Oxidative stress and antioxidant protection in the perinatal period

Shoji¹,

Koletzko

2007

Current Opinion in Clinical Nutrition &Amp; Metabolic Care

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Increased levels of oxidative stress and reduced antioxidative capacities may contribute to the pathogenesis of disorders in the perinatal period. Based on the available evidence, supplementation of pregnant women with high dosages of both vitamin C and E cannot be recommended. Asphyxiated newborn infants should be resuscitated with room air rather than with 100% oxygen. Breastfeeding appears beneficial in providing antioxidative protection.

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Effect of docosahexaenoic acid and eicosapentaenoic acid supplementation on oxidative stress levels during pregnancy

Cited by 23 publications

References 38 publications

DHA concentration of red blood cells is inversely associated with markers of lipid peroxidation in men taking DHA supplement

DHA concentration of red blood cells is inversely associated with markers of lipid peroxidation in men taking DHA supplement

Differential effects of docosahexaenoic acid on preterm and term placental pro-oxidant/antioxidant balance

Oxidative stress and antioxidant protection in the perinatal period

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