Oxidation of fish oil exacerbates alcoholic liver disease by enhancing intestinal dysbiosis in mice

Feng, Ruibing; Ma, Lijuan; Wang, Meng; Liu, Conghui; Yang, Rujie; Su, Huanxing; Yang, Yan; Wan, Jian‐Bo

doi:10.1038/s42003-020-01213-8

Cited by 29 publications

(24 citation statements)

References 52 publications

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“…IL-1β level in the cell culture medium was measured using Human ELISA Standard kits according to the manufacturer’s instruction (Cloud-Clone, Wuhan, China). H&E staining was conducted to determine the histological changes of the liver as previously described [ 36 ].…”

Section: Methodsmentioning

confidence: 99%

Red yeast rice ameliorates non-alcoholic fatty liver disease through inhibiting lipid synthesis and NF-κB/NLRP3 inflammasome-mediated hepatic inflammation in mice

2022

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Background Red yeast rice (RYR), a nutraceutical with a profound cholesterol-lowering effect, was found to attenuate non-alcoholic fatty liver disease (NAFLD) in mice. Despite monacolin K in RYR being a specific inhibitor of hydroxymethylglutaryl-coenzyme A reductase (HMCGR), the mechanisms underlying the protective effects of RYR against NAFLD are not fully elucidated. Methods Using a mouse model of high-fat diet (HFD) feeding and a cellular model of HepG2 cells challenged by lipopolysaccharide (LPS) and palmitic acid (PA), the possible molecular mechanisms were exploited in the aspects of NF-κB/NLRP3 inflammasome and mTORC1-SREBPs signaling pathways by examining the relevant gene/protein expressions. Subsequently, the correlation between these two signals was also verified using cellular experiments. Results RYR ameliorated lipid accumulation and hepatic inflammation in vivo and in vitro. RYR improved lipid metabolism through modulating mTORC1-SREBPs and their target genes related to triglyceride and cholesterol synthesis. Furthermore, RYR suppressed hepatic inflammation by inhibiting the NF-κB/NLRP3 inflammasome signaling. Interestingly, the treatment with RYR or MCC950, a specific NLRP3 inhibitor, resulted in the reduced lipid accumulation in HepG2 cells challenged by LPS plus PA, suggesting that the inhibitory effects of RYR on NLRP3 inflammasome-mediated hepatic inflammation may partially, in turn, contribute to the lipid-lowering effect of RYR. Conclusions The modulation of NF-κB/NLRP3 inflammasome and lipid synthesis may contribute to the ameliorative effects of RYR against HFD-induced NAFLD.

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Section: Methodsmentioning

confidence: 99%

Red yeast rice ameliorates non-alcoholic fatty liver disease through inhibiting lipid synthesis and NF-κB/NLRP3 inflammasome-mediated hepatic inflammation in mice

2022

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“…Dietary addition of omega-3 PUFAs increases the abundance and percentage of Bifidobacteria in the gut of male Sprague-Dawley rats [ 19 ]. EPA and DHA treatment could prevent gut microbiota dysregulation in mice [ 20 ] and increase the number of potentially beneficial lactic acid-producing bacteria and Bifidobacteria in the gut of the mice fed a high-fat diet [ 21 , 22 ]. Omega-3 PUFAs alter the abundance of beneficial intestinal bacteria, particularly Akkermansia , improve the intestinal microenvironment, increase the intestinal mucosal thickness, improve the barrier function of the intestinal mucosa, and achieve weight loss by controlling the expression of genes related to fat metabolism [ 23 ].…”

Section: Omega-3 Pufas and The Gut Microbiotamentioning

confidence: 99%

Associations among Dietary Omega-3 Polyunsaturated Fatty Acids, the Gut Microbiota, and Intestinal Immunity

Wang

et al. 2021

Mediators of Inflammation

163

120

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Omega-3 polyunsaturated fatty acids (omega-3 PUFAs), which are essential fatty acids that humans should obtain from diet, have potential benefits for human health. In addition to altering the structure and function of cell membranes, omega-3 PUFAs (docosahexaenoic acid (DHA), eicosapentaenoic acid (EPA), alpha-linolenic acid (ALA), and docosapentaenoic acid (DPA)) exert different effects on intestinal immune tolerance and gut microbiota maintenance. Firstly, we review the effect of omega-3 PUFAs on gut microbiota. And the effects of omega-3 PUFAs on intestinal immunity and inflammation were described. Furthermore, the important roles of omega-3 PUFAs in maintaining the balance between gut immunity and the gut microbiota were discussed. Additional factors, such as obesity and diseases (NAFLD, gastrointestinal malignancies or cancer, bacterial and viral infections), which are associated with variability in omega-3 PUFA metabolism, can influence omega-3 PUFAs–microbiome–immune system interactions in the intestinal tract and also play roles in regulating gut immunity. This review identifies several pathways by which the microbiota modulates the gut immune system through omega-3 PUFAs. Omega-3 supplementation can be targeted to specific pathways to prevent and alleviate intestinal diseases, which may help researchers identify innovative diagnostic methods.

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“…Research has shown that oxidized diets enhance plasma inflammatory markers and activate nuclear factor kappa B ( NF-κB ) in the small intestine, and consumption of oxidized n-3 PUFA results in 4-HHE accumulation in blood after its intestinal absorption and triggers oxidative stress and inflammation in the upper intestine ( Awada et al, 2012 ). A recent study showed that oxidized fish oil could cause intestinal dysbiosis, barrier dysfunction, and hepatic inflammation mediated by gut-derived endotoxin ( Feng et al, 2020 ). Importantly, induction of oxidative stress can be associated with intestinal inflammation and changes in host microbiota.…”

Section: Introductionmentioning

confidence: 99%

Effects of naturally oxidized corn oil on inflammatory reaction and intestinal health of broilers

Zhang

Mahmood²,

Tang

et al. 2022

Poultry Science

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This study was conducted to investigate the effects of naturally oxidized corn oil on the inflammatory reaction and intestinal health of broilers. Total 450, one-day-old Arbor Acres male broilers were randomly divided into 5 treatments with 6 replicate cages (15 birds in each replicate cage). The dietary treatment array consisted of the varying ratio of nonoxidized corn oil to naturally oxidized corn oil from 0:100, 25:75, 50:50, 75:25, and 100:0, respectively. The experimental period was 42 d. Serum, jejunum, and contents of cecum samples were taken at the age of 42 d of broilers. The results showed no significant difference in the body weight gain ( BWG ) with a different proportion of oxidized corn oil compared with the 0% oxidized oil group on d 42. The feed intake ( FI ), the concentration of immunoglobulin G ( IgG ), interferon-γ ( IFN-γ ), and interleukin-10 ( IL10 ) in serum showed a significant quadratic response with the increase of oxidized oil concentration on d 42. The serum's concentration of IgG, IFN-γ, and IL-10 reached the highest value at 75% oxidized corn oil. In addition, the mRNA expression levels of interleukin-1β ( IL-1β ), IFN-γ, nuclear factor kappa B ( NF-κB ), tumor necrosis factor α ( TNF-α ), and myeloid differentiation factor-88 ( MyD88 ) in the jejunum were significantly affected by different proportions of oxidized corn oil, and the gene expression levels were highest at 75% oxidized corn oil on d 42. The mRNA expression of Bcl2-associated X ( Bax ) in the jejunum showed a significantly quadratic curve with the increase of oxidized oil concentration, and its gene expression was the highest after adding 50% oxidized corn oil according to the regression equation on d 42. The villus height/crypt depth and goblet cells of jejunum decreased linearly with the increasing proportion of oxidized corn oil and reached the lowest point after adding 100% oxidized corn oil on d 42. The β diversity showed the remarkable differentiation of microbial communities among 5 groups, and the microbial community of the 0% oxidized oil group was significantly separated from that of 75 and 100% oxidized oil groups in the cecum. Taken together, these results showed that a low dose of naturally oxidized corn oil is not harmful to the growth of broilers, while a high dose of oxidized corn oil will trigger the inflammatory response and adversely affect the gut health of broilers.

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Oxidation of fish oil exacerbates alcoholic liver disease by enhancing intestinal dysbiosis in mice

Cited by 29 publications

References 52 publications

Red yeast rice ameliorates non-alcoholic fatty liver disease through inhibiting lipid synthesis and NF-κB/NLRP3 inflammasome-mediated hepatic inflammation in mice

Red yeast rice ameliorates non-alcoholic fatty liver disease through inhibiting lipid synthesis and NF-κB/NLRP3 inflammasome-mediated hepatic inflammation in mice

Associations among Dietary Omega-3 Polyunsaturated Fatty Acids, the Gut Microbiota, and Intestinal Immunity

Effects of naturally oxidized corn oil on inflammatory reaction and intestinal health of broilers

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