Effects of naturally oxidized corn oil on inflammatory reaction and intestinal health of broilers

Abstract

This study was conducted to investigate the effects of naturally oxidized corn oil on the inflammatory reaction and intestinal health of broilers. Total 450, one-day-old Arbor Acres male broilers were randomly divided into 5 treatments with 6 replicate cages (15 birds in each replicate cage). The dietary treatment array consisted of the varying ratio of nonoxidized corn oil to naturally oxidized corn oil from 0:100, 25:75, 50:50, 75:25, and 100:0, respectively. The experimental period was 42 d. Serum, jejunum, and contents of cecum samples were taken at the age of 42 d of broilers. The results showed no significant difference in the body weight gain ( BWG ) with a different proportion of oxidized corn oil compared with the 0% oxidized oil group on d 42. The feed intake ( FI ), the concentration of immunoglobulin G ( IgG ), interferon-γ ( IFN-γ ), and interleukin-10 ( IL10 ) in serum showed a significant quadratic response with the increase of oxidized oil concentration on d 42. The serum's concentration of IgG, IFN-γ, and IL-10 reached the highest value at 75% oxidized corn oil. In addition, the mRNA expression levels of interleukin-1β ( IL-1β ), IFN-γ, nuclear factor kappa B ( NF-κB ), tumor necrosis factor α ( TNF-α ), and myeloid differentiation factor-88 ( MyD88 ) in the jejunum were significantly affected by different proportions of oxidized corn oil, and the gene expression levels were highest at 75% oxidized corn oil on d 42. The mRNA expression of Bcl2-associated X ( Bax ) in the jejunum showed a significantly quadratic curve with the increase of oxidized oil concentration, and its gene expression was the highest after adding 50% oxidized corn oil according to the regression equation on d 42. The villus height/crypt depth and goblet cells of jejunum decreased linearly with the increasing proportion of oxidized corn oil and reached the lowest point after adding 100% oxidized corn oil on d 42. The β diversity showed the remarkable differentiation of microbial communities among 5 groups, and the microbial community of the 0% oxidized oil group was significantly separated from that of 75 and 100% oxidized oil groups in the cecum. Taken together, these results showed that a low dose of naturally oxidized corn oil is not harmful to the growth of broilers, while a high dose of oxidized corn oil will trigger the inflammatory response and adversely affect the gut health of broilers.

Short-term feeding of defatted bovine colostrum mitigates inflammation in the gut via changes in metabolites and microbiota in a chicken animal model

“…Additionally, epithelial cells can have levels of NT proteins with cell function deficits that vary by diet in the absence of detectable infectious disease [ 26 , 27 ]. Oxidative/nitration protein and lipid damage has been generated in chickens simply by low levels of partially oxidized corn oil or polyunsaturated fatty acids being in the diet [ 38 ]; similarly, that oxidized oil-induced inflammation was relieved with the addition of the flavonoid antioxidant quercetin to the chicken diet [ 39 ]. Mitigation of gut dysfunction by reducing inflammation is a goal of both human and veterinary medicine.…”

Selenium Nanoparticles Improved Intestinal Health Through Modulation of the NLRP3 Signaling Pathway

“…Ali et al ( 28 ) found that the reduction of claudin-1, ZO-1, and ZO-2 by SeNPs might reduce the sites to attaching the pathogen bacteria. Meanwhile, the products of oxidative stress also increased intestinal permeability by decreasing expression levels of tight junction proteins ( 19 , 37 ). The increase of Se in the diet reduced the influence of heat stress on the integrity of the intestinal barrier by reducing oxidative stress products ( 38 ).…”

“…Under the Leica microscope (DMI803250593, Heidelberg, Germany), the intestinal villus height (VH) and crypt depth (CD) were measured by randomly selecting 10 intact villi for every bird. The density of goblet cells was expressed as the number of goblet cells per 100 μm ( 19 ). The method was calculated by randomly selecting 10 intact villi and measuring the number of goblet cells in corresponding villi under the mid-villi area (100 μm in length) ( 19 ).…”

“…The density of goblet cells was expressed as the number of goblet cells per 100 μm ( 19 ). The method was calculated by randomly selecting 10 intact villi and measuring the number of goblet cells in corresponding villi under the mid-villi area (100 μm in length) ( 19 ).…”

R- Is Superior to S-Form of α-Lipoic Acid in Anti-Inflammatory and Antioxidant Effects in Laying Hens

“…In animal experimental studies, oxidative stress models are generally constructed by adding oxidized oils, including oxidized fish oil, oxidized corn oil, and oxidized soybean oil. In a test of the effect of using different ratios of oxidized corn oil on broiler chickens, Zhang et al found that serum and jejunal antioxidant factors showed a significant quadratic response with the increase in oxidized oil concentration [ 37 ]. Another trial using oxidized tuna oil as a stressor found that dietary oxidized tuna oil increased some lipid peroxidation markers in trout [ 38 ].…”