Oxidation of Ethylene Glycol by a Salt-Requiring Bacterium

Caskey, William H.; Taber, Willard A.

doi:10.1128/aem.42.1.180-183.1981

Cited by 12 publications

(7 citation statements)

References 6 publications

(10 reference statements)

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“…Under aerobic conditions, PEG is degraded by dehydrogenation to either carboxylated intermediates (15,16) or glycolaldehyde (26), by acetaldehyde production (20), and by extracellular hydrolytic cleavage with production of ethylene glycol (EG) and diethylene glycol (11). Monomeric EG is degraded either by oxidation to glycolic acid with further metabolism through the glycerate pathway (4,10,29) or by dehydration to acetaldehyde with metabolism through the tricarboxylic acid cycle (28).…”

mentioning

confidence: 99%

Metabolism of polyethylene glycol by two anaerobic bacteria, Desulfovibrio desulfuricans and a Bacteroides sp

Dwyer¹,

Tiedje²

1986

Appl Environ Microbiol

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Two anaerobic bacteria were isolated from polyethylene glycol (PEG)-degrading, methanogenic, enrichment cultures obtained from a municipal sludge digester. One isolate, identified as Desulfovibrio desulfuricans (strain DG2), metabolized oligomers ranging from ethylene glycol (EG) to tetraethylene glycol. The other isolate, identified as a Bacteroides sp. (strain PG1), metabolized diethylene glycol and polymers of PEG up to an average molecular mass of 20,000 g/mol [PEG 20000; HO-(CH2-CH2-O-).HJ. Both strains produced acetaldehyde as an intermediate, with acetate, ethanol, and hydrogen as end products. In coculture with a Methanobacterium sp., the end products were acetate and methane. Polypropylene glycol [HO-(CH2-CH2-CH2-O-),H] was not metabolized by either bacterium, and methanogenic enrichments could not be obtained on this substrate. Cell extracts of both bacteria dehydrogenated EG, PEGs up to PEG 400 in size, acetaldehyde, and other monoand dihydroxylated compounds. Extracts of Bacteroides strain PG1 could not dehydrogenate long polymers of PEG (.1,000 g/mol), but the bacterium grew with PEG 1000 or PEG 20000 as a substrate and * Corresponding author. t Journal article 11973 of the Michigan Agricultural Experiment Station.

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confidence: 99%

Metabolism of polyethylene glycol by two anaerobic bacteria, Desulfovibrio desulfuricans and a Bacteroides sp

Dwyer¹,

Tiedje²

1986

Appl Environ Microbiol

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“…These find use as reactive building blocks in the production of agro-, aroma-, and polymer chemicals, or pharmaceuticals (Sajtos, 1991;Mattioda and Christidis, 2000;Yue et al, 2012). Several microorganisms have been reported to utilize EG such as those from Acetobacter and Gluconobacter (DeLey and Kersters, 1964), Acinetobacter and halophilic bacterium, T-52 (ATCC 27042) (Gonzalez et al, 1972;Caskey and Taber, 1981), Flavobacterium species (Child and Willetts, 1978), Hansenula (Harada and Hirabayashi, 1968), Candida, Pichia naganishii AKU4267, and Rhodotorula sp. 3Pr-126 (Kataoka et al, 2001).…”

Section: Pet Degrading Microorganismsmentioning

confidence: 99%

Microbial and Enzymatic Degradation of Synthetic Plastics

Montazer²,

et al. 2020

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Synthetic plastics are pivotal in our current lifestyle and therefore, its accumulation is a major concern for environment and human health. Petroleum-derived (petro-)polymers such as polyethylene (PE), polyethylene terephthalate (PET), polyurethane (PU), polystyrene (PS), polypropylene (PP), and polyvinyl chloride (PVC) are extremely recalcitrant to natural biodegradation pathways. Some microorganisms with the ability to degrade petro-polymers under in vitro conditions have been isolated and characterized. In some cases, the enzymes expressed by these microbes have been cloned and sequenced. The rate of polymer biodegradation depends on several factors including chemical structures, molecular weights, and degrees of crystallinity. Polymers are large molecules having both regular crystals (crystalline region) and irregular groups (amorphous region), where the latter provides polymers with flexibility. Highly crystalline polymers like polyethylene (95%), are rigid with a low capacity to resist impacts. PET-based plastics possess a high degree of crystallinity (30–50%), which is one of the principal reasons for their low rate of microbial degradation, which is projected to take more than 50 years for complete degraded in the natural environment, and hundreds of years if discarded into the oceans, due to their lower temperature and oxygen availability. The enzymatic degradation occurs in two stages: adsorption of enzymes on the polymer surface, followed by hydro-peroxidation/hydrolysis of the bonds. The sources of plastic-degrading enzymes can be found in microorganisms from various environments as well as digestive intestine of some invertebrates. Microbial and enzymatic degradation of waste petro-plastics is a promising strategy for depolymerization of waste petro-plastics into polymer monomers for recycling, or to covert waste plastics into higher value bioproducts, such as biodegradable polymers via mineralization. The objective of this review is to outline the advances made in the microbial degradation of synthetic plastics and, overview the enzymes involved in biodegradation.

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“…Tabor reported additional data on the salt-requiring bacterium, T-52 (Caskey and Tabor, 1981). Cells grown on EG had elevated enzyme activities for EG dehydrogenase and glycolate oxidase.…”

Section: A-7mentioning

confidence: 99%