Metabolism of polyethylene glycol by two anaerobic bacteria, Desulfovibrio desulfuricans and a Bacteroides sp

Dwyer, Daryl F.; Tiedje, James M.

doi:10.1128/aem.52.4.852-856.1986

Cited by 87 publications

(40 citation statements)

References 31 publications

(33 reference statements)

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“…In line with this, it has been found that several bacteria are able to use PPGs as carbon and energy source [2]. Since most PEG-utilizing bacteria do not grow on PPG [3,4], the biological degradation pathways of PEG and PPG could be dissimilar.…”

Section: Introductionmentioning

confidence: 99%

Involvement of a quinoprotein (PQQ-containing) alcohol dehydrogenase in the degradation of polypropylene glycols by the bacterium Stenotrophomonas maltophilia

Tachibana

2003

FEMS Microbiology Letters

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Previous work has shown that when the bacterium Stenotrophomonas maltophilia is grown on polypropylene glycol, different dye-linked polypropylene glycol dehydrogenase (PPG-DH) activities are induced during growth. Here the purification and characterization of the dehydrogenase activity induced in the stationary phase, and present in the periplasmic space, is described. The homogeneous enzyme preparation obtained consists of a homodimeric protein with a molecular mass of about 123 kDa and an isoelectric point of 5.9. The cofactor of the enzyme appeared to be pyrroloquinoline quinone (PQQ), no heme c was present, and holo-enzyme contained two PQQ molecules per enzyme molecule. In these respects, PPG-DH described here is similar to already known quinoprotein alcohol dehydrogenases, but in other respects, it is different. Therefore, it is suggested that PPG-DH could be a new type of quinoprotein alcohol dehydrogenase. Based on its strong preference for polyols, PPG-DH seems well fitted to carry out the first step in the degradation of PPGs, synthetic polymers containing a variety of hydroxyl groups.

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Section: Introductionmentioning

confidence: 99%

Involvement of a quinoprotein (PQQ-containing) alcohol dehydrogenase in the degradation of polypropylene glycols by the bacterium Stenotrophomonas maltophilia

Tachibana

2003

FEMS Microbiology Letters

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“…Several acetogenic (4-6, 9-11, 16, 19, 22) and nonacetogenic (8,12,13,20) bacteria cleave aromatic and aliphatic 0-methyl ethers under anaerobic conditions. These growth experiments were carried out after the first report on the selective isolation of Acetobacterium woodii with methoxybenzoates as carbon sources was published (1).…”

mentioning

confidence: 99%

Corrinoid-Dependent Methyl Transfer Reactions Are Involved in Methanol and 3,4-Dimethoxybenzoate Metabolism by Sporomusa ovata

Stupperich

Konle

1993

Appl Environ Microbiol

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Washed and air-oxidized proteins from Sporomusa ovata cleaved the CO bond of methanol or methoxyaromatics and transferred the methyl to DL-tetrahydrofolate. The reactions strictly required a reductive activation by titanium citrate, catalytic amounts of ATP, and the addition of DL-tetrahydrofolate. Methylcorrinoidcontaining proteins carried the methanol methyl, which was transferred to DL-tetrahydrofolate at a specific rate of 120 nmol h-' mg of protein-'. Tetrahydrofolate methylation diminished after the addition of 1-iodopropane or when the methyl donor methanol was replaced by 3,4-dimethoxybenzoate. However, whole Sporomusa cells utilize the methoxyl groups of 3,4-dimethoxybenzoate as a carbon source by a sequential 0 demethylation to 4-hydroxy-3-methoxybenzoate and 3,4-dihydroxybenzoate. The in vitro 0 demethylation of 3,4-[4-methazyl-14C]dimethoxybenzoate proceeded via two distinct corrinoid-containing proteins to form 5-[14C]methyltetrahydrofolate at a specific rate of 200 nmol h-1 mg of protein-'. Proteins from 3,4-dimethoxybenzoategrown cells efficiently used methoxybenzoates with vicinal substituents only, but they were unable to activate methanol. These results emphasized that specific enzymes are involved in methanol activation as well as in the activation of various methoxybenzoates and that similar corrinoid-dependent methyl transfer pathways are employed in 5-methyl-tetrahydrofolate formation from these substrates. Methyl-tetrahydrofolate could be demethylated by a distinct methyl transferase. That enzyme activity was present in washed and air-oxidized cell extracts from methanol-grown cells and from 3,4-dimethoxybenzoate-grown cells. It used cob(I)alamin as the methyl acceptor in vitro, which was methylated at a rate of 48 nmol min-' mg of protein-' even when ATP was omitted from the assay mixture. This methyl-cob(III)alamin formation made possible a spectrophotometric quantification of the preceding methyl transfers from methanol or methoxybenzoates to DL-tetrahydrofolate.

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“…Isolated from a municipal sludge digester, it could metabolize diethylene glycol and polyethylene glycol polymers into acetate, ethanol, and hydrogen. 29 These two species could play their roles in complex biopolymer degradation. Pseudomonas veronii strain might be responsible for bacterial self-aggregation and may accelerate granules formation and integrity.…”

Section: Identification Of Dominant Species In the Agsmentioning

confidence: 99%

Effects of influent loads on performance and microbial community dynamics of aerobic granular sludge treating piggery wastewater

Chen

Huang

Tian

et al. 2017

J of Chemical Tech & Biotech

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BACKGROUND Understanding how influent loads affect the microbial community may promote applications of aerobic granular sludge (AGS). Herein, performance and microbial community dynamics of the AGS were assessed with stepwise increasing influent loads. RESULTS AGS exhibited good structure stability and effective removal efficiency; when the influent load increased from 6.5 kg COD m‐3 d‐1 (0.255 NH4+‐N m‐3 d‐1) to 10.1 kg COD m‐3 d‐1 (0.455 kg NH4+‐N m‐3 d‐1). COD and NH4+‐N removal efficiencies reached 97.1–97.9% and 84.5–98%, respectively. As indicated from the PCR‐DGGE results, the microbial community and dominant species in the AGS also gradually evolved along with the increase of influent loads. A coincidence was observed, namely, three main distinct clusters of the phylogenetic tree appeared respectively in the three stages of influent loads. The microbial diversity attained a maximum in the medium loads stage. The identified bacteria mainly belonged to Proteobacteria, Actinobacteria, Bacteroidetes and Firmicutes. The dominant species were responsible for the AGS stability and pollutant removal. CONCLUSION Variation in the influent load strongly affected the microbial community dynamics, and hence the AGS performance. These results and understanding could lead to potential improvements in guiding AGS wastewater treatment. © 2017 Society of Chemical Industry

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Metabolism of polyethylene glycol by two anaerobic bacteria, Desulfovibrio desulfuricans and a Bacteroides sp

Cited by 87 publications

References 31 publications

Involvement of a quinoprotein (PQQ-containing) alcohol dehydrogenase in the degradation of polypropylene glycols by the bacterium Stenotrophomonas maltophilia

Involvement of a quinoprotein (PQQ-containing) alcohol dehydrogenase in the degradation of polypropylene glycols by the bacterium Stenotrophomonas maltophilia

Corrinoid-Dependent Methyl Transfer Reactions Are Involved in Methanol and 3,4-Dimethoxybenzoate Metabolism by Sporomusa ovata

Effects of influent loads on performance and microbial community dynamics of aerobic granular sludge treating piggery wastewater

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