Oxidation of dobutamine and dopamine by horseradish peroxidase

In vitro Production of Hemin‐Based Artificial Metalloenzymes

“…The kinetic profile of the peroxidation of dopamine was revealed by fluorescence and UV‐Vis measurements, yielding similar results (Figure S11). We determined a K M value of 43±11 μM, which is significantly low compared to other natural enzymes such as catechol oxidases, and other peroxidases, with values that range in the mM [35–37] . Finally, we also observed that the ArM outperformed the uncoordinated metalorganic porphyrin in terms of TOF, with a value of 0.22±0.01 s −1 compared to 0.099±0.005 s −1 , respectively (Figure S12, Table S2) [38] .…”

“…We determined a K M value of 43 � 11 μM, which is significantly low compared to other natural enzymes such as catechol oxidases, and other peroxidases, with values that range in the mM. [35][36][37] Finally, we also observed that the ArM outperformed the uncoordinated metalorganic porphyrin in terms of TOF, with a value of 0.22 � 0.01 s À 1 compared to 0.099 � 0.005 s À 1 , respectively (Figure S12, Table S2). [38] Due to the significantly higher affinity of CTPR3 Hhemin towards dopamine compared to other systems (Table S1), the limited autoxidation of dopamine in the absence of the catalysts (Figure S13), and the significant difference in the catalytic rates compared to free hemin, we concluded that dopamine is a convenient substrate for the droplet-based screening developed in this work.…”

Electrochemical transformations catalyzed by cytochrome P450s and peroxidases

“…HRP can catalyze oxidation of organic compounds like phenols, alcohols, aldehydes, and chlorinated organics. 60,[365][366][367][368][369][370][371][372] HRP can be covalently immobilized on beads. The covalently bound HRP can be activated by H 2 O 2 produced electrochemically to catalyze the oxidation of organic compounds.…”