Oxidation of Benzo(a) Pyrene Catalyzed by Phenol Oxidases of Alga Nitella sp. and Potato Tubers

Abstract

Sunzmary:The kinetics of oxidation of carcinogenic benzo(a)pyrene (0.1 nM solution in phosphate buffer) catalyzed by enzyme protein of soil and water plants (potato tubers and alga Nitetla sp.) has been studied. The benzo(a)pyrene oxidation rate is determined by the phenoloxidase activity of the enzyme protein from both sources to a n equal degree and expressed by the Michaelis-Menten equation. The values for kcat and apparent Km were (0.97k0.14) Ms-1 and 33+GyM, respectively.The presence of other compounds (including protein substances) in acetone preparations of potato tubers considerably suppresses its catalytic activity in relation to benzo(a)pyrene.Consequently, in the polluted biosphere some water plants. such as alga Nitella sp., and potato tuber can transform carcinogenic benzo(a)pyrene (BP).As oxidation products, all three BP-quinoncs, i.e. 1,6-, 3,6and 6,12-diones, were identified.

Выделение Ферментного Препарата Из Водорослей Балтийского Моря Chara Aspera

“…Нами показана также роль макроводорослей в окислительном превращении ПАУ [ 3 ]. Можно полагать, что эти процессы катализируются оксидоредуктазными ферментами, которые, как установлено [ 4 ], способствуют деградации классического ПАУ бенз (а)пирена (БП). Отсюда вытекает цель настоящей работы: выделение ферментного белка из макроводорослей и проверка его активности относительно окислительной деградации БП.…”