Mutualism – the disposition to cooperate in ways that benefit both actor and recipient – has been proposed as a key construct in the evolution of cooperation, with distinct adaptations for 1) partner choice, 2) division, 3) punishment, and 4) helping. However, no psychological measure or validation of this psychological structure exists. To fill this need, in two pre-registered studies (total N = 902), we: (A) Develop and administer items assessing each of the four mutualist adaptations; (B) Show good fit to the predicted four factor model; (C) Demonstrate reliability and stability across time; (D) Evidence discriminant validity from existing constructs, including compassion and utilitarianism; (E) Establish external validity by predicting proportional choices in catch division, opposition to partner coercion, and reduced support for redistribution; and (F) Replicate each of these findings. Jointly, these results support the validity of mutualism, including a motive to maintain the freedom to choose, and provide reliable scales for use in integrating, further developing, and applying mutualism.