Oxacillin-susceptible methicillin-resistant Staphylococcus aureus (OS-MRSA), a hidden resistant mechanism among clinically significant isolates in the Wessex region/UK

Saeed, Kordo; Ahmad, Nusreen; Dryden, Matthew; Cortes, Nicholas; Marsh, Peter; Sitjar, A.; Wyllie, Sarah; Bourne, S.; Hemming, J.; Jeppesen, C.; Green, S.

doi:10.1007/s15010-014-0641-1

Cited by 40 publications

(34 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…These results were not consistent with a similar study done in Nigeria that 25% of cases were resistant to amoxicillin-clavulanic acid and 23% to erythromycin [19]. The difference observed between resistance rate to cefoxitin (11/60), and frequency of mecA (36/60) was related to the spread of the cefoxitin/oxacillin susceptible mecA positive OS-MRSA isolates in healthy population consistent with previous studies [20,21].…”

Section: Discussioncontrasting

confidence: 71%

The Prevalence of ACME-arcA and PVL Genes Among Staphylococcus Aureus Isolates in a Student Population from North-West of Iran

Dehnad¹,

Rad²,

Rahbarnia³

et al. 2020

Preprint

View full text Add to dashboard Cite

Objectives: Staphylococcus aureus (S. aureus) is the most prevalent cause of skin infections, especially in colonized individuals. Panton–Valentine leukocidin (PVL) and Arginine catabolic mobile element (ACME) are known as the most common virulence factors of S. aureus. This cross-sectional study was conducted to examine the prevalence of ACME-arcA and PVL genes among S.aureus isolates in the student population. Nasal swab samples were randomly collected from 400 healthy students from Tabriz, Iran. The antibiotic resistance pattern of S.aureus isolates was examined by the disk diffusion method. The presence of ACME-arcA, PVL, and mecA genes was detected by PCR reaction.Results: overall, 15% (60/400) students were nasal carriage of S. aureus and 2.75 % (11/400) were MRSA carriage. The frequency of mecA, ACME-arcA, and PVL genes was 54.54% (36/60), 46.66% (28/60), and 16.66% (10/60) respectively. The prevalence of ACME-arcA and PVL genes was independent of gender (P =0.142, P=0.337, respectively). A notable association was observed between the existence of ACME-arcA gene and the frequency of mecA gene (P <0.05), while the incidence of PVL was independent on mecA. These findings highlight the necessity of monitoring nasal carriers in a healthy community to prevent subsequent infections.

show abstract

Section: Discussioncontrasting

confidence: 71%

The Prevalence of ACME-arcA and PVL Genes Among Staphylococcus Aureus Isolates in a Student Population from North-West of Iran

Dehnad¹,

Rad²,

Rahbarnia³

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

“…Vyletelova et al (2011a) confirmed 23 isolated strains as MRSA by the PCR method (mecA gene), but 25 strains were resistant to oxacillin by the disk diffusion method. Saeed et al (2014) also found six (1.2%) OS-MRSA isolates phenotypically identified and reported as MRSA by conventional methods, originated from human samples. The occurrence of OS-MRSA means a hidden resistant mechanism that can have the impact on the correct treatment of S. aureus infections.…”

Section: Discussionmentioning

confidence: 86%

Occurrence of Staphylococcus aureus in cattle, sheep, goat, and pig rearing in the Czech Republic

et al. 2017

View full text Add to dashboard Cite

The study focused on the prevalence of Staphylococcus aureus and resistant strains in livestock. In this study, 114 different samples from three cattle farms (84 from two farms of dairy cows and 30 from one farm of suckler cows), 132 samples from one sheep farm, 120 samples from one goat farm, and 82 samples from three pig farms were examined. Strains identified as Staphylococcus aureus were further analysed by the polymerase chain reaction method for detection of the mecA gene and for confirmation of the sequence type 398. Positive incidence of Staphylococcus aureus was confirmed in farms of suckler cows, sheep, goats and pigs. The incidence of methicilinresistant Staphylococcus aureus was confirmed at a goat farm, with all strains belonging to the sequence type 398. Repetitive element palindromic-polymerase chain reaction analysis was performed to compare the relatedness of selected human and animal S. aureus strains at the goat and sheep farms. The obtained data from repetitive element-polymerase chain reaction analysis showed significant clonal similarity among the tested isolates and indicated the possibility of mutual transmission between animals or animal and human and possible transfer in the food chain. Breeding environment, food animals, methicillin resistant S. aureus

show abstract

“…The occurrence of OS-MRSA also raises concerns about optimal therapeutics to target infections. It is commonly believed that most OS-MRSA strains exhibit hetero-resistance to oxacillin, which results in treatment failure of β-lactam antibiotics [8]. Notably, mupirocin could lead to conversion of OS-MRSA from a heterogeneous into a highly homogeneous resistant phenotype [34].…”

Section: Optimized Detection Procedures For Os-mrsamentioning

confidence: 99%

“…organisms in S. aureus, such as oxacillin-susceptible MRSA (OS-MRSA), which carry mecA or mecC but show MIC of oxacillin in the susceptible range (≤2 mg/L), have been described worldwide over recent years [7][8][9][10]. However, the prevalence of OS-MRSA in Chinese hospitals was reported to be 1.6% (34 of 2068) only in a single study [10], limited study has investigated the characteristics of OS-MRSA currently prevalent within Chinese hospitals and offered insights into the management of this special group of MRSA for clinical laboratories.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Current status of oxacillin-susceptible mecA-positive Staphylococcus aureus infection in Shanghai, China: A multicenter study

Liu

Zhong

et al. 2019

Preprint

View full text Add to dashboard Cite

The worldwide reported oxacillin-susceptible mecA -positive Staphylococcus aureus (OS-MRSA) represents a distinctly important challenge to detection and treatment of MRSA, but finite data on current status of OS-MRSA infection in Chinese hospitals are available. The present multicenter study carried out a battery of phenotypic susceptibility tests as well as diagnostic tests (PBP2a detection, mecA , and mecC PCR) for a collection of 956 clinical S. aureus isolates from 10 hospitals in Shanghai, molecular typing was performed for all identified OS-MRSA strains. OS-MRSA represented 1.8% (17/956) of total isolates and were commonly borderline oxacillin-susceptible (Oxacillin-MIC of 1 or 2 mg/L). 10 of 17 OS-MRSA were ST59 lineages, followed by ST965 (3/17). Unlike oxacillin-resistant MRSA that commonly exhibit a multidrug resistant (MDR) phenotype, OS-MRSA were less likely to be MDR and displayed MIC pattern remarkably differential from OR-MRSA. OS-MRSA showed oxacillin-inducible oxacillin resistance and the majority of them (15/17) were cefoxitin-resistant by cefoxitin disk diffusion. S. aureus with borderline susceptible oxacillin MICs (1 or 2 mg/L) should be confirmed by cefoxitin disk diffusion in clinical practice, whereas susceptible isolates reclassified by cefoxitin disk diffusion should still be subjected to PBP2a testing and (or) mecA (mecC) PCR. The presented study has characterized phenotypically and molecularly an atypical type of MRSA showing cryptic but inducible resistance to oxacillin, but its underlying mechanisms warrant further elucidation.

show abstract

Oxacillin-susceptible methicillin-resistant Staphylococcus aureus (OS-MRSA), a hidden resistant mechanism among clinically significant isolates in the Wessex region/UK

Cited by 40 publications

References 31 publications

The Prevalence of ACME-arcA and PVL Genes Among Staphylococcus Aureus Isolates in a Student Population from North-West of Iran

The Prevalence of ACME-arcA and PVL Genes Among Staphylococcus Aureus Isolates in a Student Population from North-West of Iran

Occurrence of Staphylococcus aureus in cattle, sheep, goat, and pig rearing in the Czech Republic

Current status of oxacillin-susceptible mecA-positive Staphylococcus aureus infection in Shanghai, China: A multicenter study

Contact Info

Product

Resources

About