Oxacillin Resistance and Antimicrobial Susceptibility Profile of Staphylococcussaprophyticus and Other Staphylococci Isolated from Patients with Urinary Tract Infection

Ferreira, Adriano Martison; Bonesso, Mariana Fávero; Mondelli, Alessandro Lia; Camargo, Carlos Henrique; Cunha, Maria de Lourdes Ribeiro de Souza da

doi:10.1159/000346529

Cited by 25 publications

(18 citation statements)

References 46 publications

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“…In addition, 30.1% of the isolates were resistant to erythromycin, 23.0% to gentamicin, and 15.3% to sulfamethoxazole/trimethoprim. Our results on oxacillin resistance are consistent with those reported by Ferreira et al [ 17 ]. Oxacillin resistance was also observed in the single S. hominis isolate of the present study and in one of the three S. warneri isolates.…”

Section: Discussionsupporting

confidence: 93%

Antimicrobial Resistance Profile of Planktonic and Biofilm Cells of Staphylococcus aureus and Coagulase-Negative Staphylococci

Oliveira

Pereira

Pinheiro

et al. 2016

IJMS

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The objective of the present study was to determine the antimicrobial resistance profile of planktonic and biofilm cells of Staphylococcus aureus and coagulase-negative staphylococci (CoNS). Two hundred Staphylococcus spp. strains were studied, including 50 S. aureus and 150 CoNS strains (50 S. epidermidis, 20 S. haemolyticus, 20 S. warneri, 20 S. hominis, 20 S. lugdunensis, and 20 S. saprophyticus). Biofilm formation was investigated by adherence to polystyrene plates. Positive strains were submitted to the broth microdilution method to determine the minimum inhibitory concentration (MIC) for planktonic and biofilm cells and the minimal bactericidal concentration for biofilm cells (MBCB). Forty-nine Staphylococcus spp. strains (14 S. aureus, 13 S. epidermidis, 13 S. saprophyticus, 3 S. haemolyticus, 1 S. hominis, 3 S. warneri, and 2 S. lugdunensis) were biofilm producers. These isolates were evaluated regarding their resistance profile. Determination of planktonic cell MIC identified three (21.4%) S. aureus strains that were resistant to oxacillin and six (42.8%) that were resistant to erythromycin. Among the CoNS, 31 (88.6%) strains were resistant to oxacillin, 14 (40%) to erythromycin, 18 (51.4%) to gentamicin, and 8 (22.8%) to sulfamethoxazole/trimethoprim. None of the planktonic isolates were resistant to vancomycin or linezolid. MICs were 2-, 4-, 8-, and up to 16-fold higher for biofilm cells than for planktonic cells. This observation was more common for vancomycin and erythromycin. The MBCB ranged from 8 to >256 µg/mL for oxacillin, 128 to >128 µg/mL for vancomycin, 256 to >256 µg/mL for erythromycin and gentamicin, >64 µg/mL for linezolid, and 32/608 to >32/608 µg/mL for sulfamethoxazole/trimethoprim. The results showed considerably higher MICs for S. aureus and CoNS biofilm cells compared to planktonic cells. Analysis of MBCM confirmed that even high concentrations of vancomycin were unable to eliminate the biofilms of S. aureus and CoNS species. Linezolid was the most effective drug in inhibiting staphylococci in the biofilm, without an increase in the MIC, when compared to planktonic cells. None of the isolates were resistant to this drug.

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Section: Discussionsupporting

confidence: 93%

Antimicrobial Resistance Profile of Planktonic and Biofilm Cells of Staphylococcus aureus and Coagulase-Negative Staphylococci

Oliveira

Pereira

Pinheiro

et al. 2016

IJMS

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“…Although 3 S. epidermidis strains were linezolid susceptible (MIC: 2-4 mg/l), they were positive for the presence of the C2534T mutation. In a study by Ferreira et al [14] among 101 staphylococci phenotypically tested, the range of linezolid MIC was 0.5-4 mg/l, but no molecular analysis was performed. In another study, linezolid nonsusceptibility among staphylococci was associated with the G2576T mutation in 23S rDNA, whereas 5 isolates carried the mobile cfr element [15].…”

Section: Discussionmentioning

confidence: 99%

Factors Influencing Linezolid-Nonsusceptible Coagulase-Negative Staphylococci Dissemination Among Patients in the Intensive Care Unit: A Retrospective Cohort Study

Papadimitriou-Olivgeris

Giormezis

Fligou³

et al. 2013

Chemotherapy

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Background: The aim of the present study was to identify risk factors for linezolid-nonsusceptible coagulase-negative staphylococci (CNS) dissemination in the intensive care unit. Methods: Among the 246 patients included, 33 revealed a linezolid-nonsusceptible CNS-positive culture specimen, 68 were positive for linezolid-susceptible CNS and 145 served as controls. Isolates were characterized by phenotypic and genotypic methods to species level, susceptibility to antistaphylococcal agents and clones. Results: Among the 33 linezolid-nonsusceptible CNS patients, 29 revealed Staphylococcus epidermidis and 4 Staphylococcus capitis. All S. epidermidis strains belonged to the ST22 clone (by multilocus sequence typing), 26 carried both C2534T and T2504A and 3 strains were C2543T mutations. S. capitis strains were stratified as a common pulsed-field gel electrophoresis type and carried the G2576T mutation. Risk factors for linezolid-nonsusceptible CNS isolation were linezolid administration and mean number of linezolid-nonsusceptible CNS-positive patients in nearby beds per day. Conclusions: These results reinforce the aspect of rational antibiotic usage, but also highlight the need for strict infection control measures to prevent the dissemination of linezolid-nonsusceptible CNS.

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“…is not only because of its pathogenicity but these species show high resistance in the treatment of UTIs (Ferreira et al, 2012;Lunacek et al, 2014). Hence, considering the situation of this pathogenic agents and evaluating its risk factors are of great importance.…”

Section: Introductionmentioning

confidence: 99%

Incidence and Antibiotic Susceptibility Pattern of Staphylococcus spp. in Urinary Tract Infections (UTI), IRAN, 2013-2014

Erfani¹,

Ghasemi²,

Mirnejad³

et al. 2015

Current Research in Bacteriology

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The persistence of this study is to investigate the pattern of antibiotic resistance Staphylococcus spp. causing urinary tract infection in 1675 of samples in common pathogens that cause urinary tract infection. This study is the first to evaluate the incidence of antibiotic resistance is such a large number of samples in Iran. The susceptibility of samples obtained from 14332 patients with urinary tract infections admitted to different medical diagnostic laboratories of Iran, was measured using disk diffusion method for 18 common antibiotics and multiplex PCR for Staphylococcus identification. Most of the identified Staphylococcus spp. were Staphylococcus saprophyticus (949 samples) and Staphylococcus epidermidis (481 samples). The most resistant antibiotics were identified as methicillin and penicillin, 84.1 and 94.2%, respectively. The findings of this study indicate that Staphylococcus spp. were second cause of common infection among outpatients. There are also bacteria with high resistance that is but interfere with prescription of drugs in order to treat urinary tract system infection. Also increasing of resistance to drugs among bacterial pathogens is evolving and requires an inspectoral and research procedure which could provide more information for doctors to treat the infection more efficiently.

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Oxacillin Resistance and Antimicrobial Susceptibility Profile of Staphylococcussaprophyticus and Other Staphylococci Isolated from Patients with Urinary Tract Infection

Cited by 25 publications

References 46 publications

Antimicrobial Resistance Profile of Planktonic and Biofilm Cells of Staphylococcus aureus and Coagulase-Negative Staphylococci

Antimicrobial Resistance Profile of Planktonic and Biofilm Cells of Staphylococcus aureus and Coagulase-Negative Staphylococci

Factors Influencing Linezolid-Nonsusceptible Coagulase-Negative Staphylococci Dissemination Among Patients in the Intensive Care Unit: A Retrospective Cohort Study

Incidence and Antibiotic Susceptibility Pattern of Staphylococcus spp. in Urinary Tract Infections (UTI), IRAN, 2013-2014

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