Ovotransferrin Antibacterial Peptide Coupling Mesoporous Silica Nanoparticle as an Effective Antibiotic Delivery System for Treating Bacterial Infection In Vivo

Abstract

Antibiotic-resistant pathogens are a serious threat to global public health. The emergence of drug-resistant pathogens is due to the improper use of antibiotics, making the treatment of bacterial infections very challenging. Here, we reported an efficient antibiotic delivery nanoparticle to minimize antibiotic resistance. The nanoparticle was designed to target the bacterial membrane using mesoporous silica nanoparticles (MSNs) modified with an ovotransferrin-derived antimicrobial peptide (OVTp12), enabling the antibiotic to be delivered to the vicinity of the pathogenic bacteria. Moreover, we observed that OVTp12-modified nanoparticles effectively inhibited the growth of Escherichia coli in vitro and in vivo. The nanoparticle with high biosafety could significantly downregulate the expression of inflammation-related cytokines in infected tissues. Thus, this novel bacterial targeted nanoparticle provides advantages in minimizing bacterial drug resistance and treating bacterial infection.

Recent Advances and Mechanistic Insights into Antibacterial Activity, Antibiofilm Activity, and Cytotoxicity of Silver Nanoparticles

“…Recently, several antimicrobial agents such as polymeric cations, − antimicrobial peptides, − photodynamic or photothermal agents, − quaternary ammonium-based compounds, , and inorganic NPs − were reported. The rapid development in nanomaterials has set up a new ambit in the biomedical field and manifested possibilities in the research based on bacterial infections. − Efforts are forged to generate green synthesized nanomaterials with different metals like zinc, copper, titanium, , gold, , and silver. , Among them, inorganic NPs (e.g., copper, silver, and gold NPs) brought new practicability in the development of efficient and safe antimicrobial agents. − Especially, silver nanoparticles (AgNPs), an extensive antibacterial agent, ,− have recently fascinated much attention due to their excellent antimicrobial upshot on drug-resistant bacteria − and auspicious germicidal potency in bacterial infections. , The silver ions (Ag + ) discharged from AgNPs play a pivotal role in antimicrobial activity , by enhancing the membrane permeability and producing reactive oxygen species (ROS) to damage cell walls. − However, the tendency of aggregation of AgNPs and disturbance of Ag + release in the aqueous solution may diminish the antimicrobial efficiency of AgNPs during long-term use. ,− As a consequence, Ag-incorporated nanocomposites with high stability have aroused significant interest as bactericidal agents. − ...…”

Advances of Antimicrobial Peptide‐Based Biomaterials for the Treatment of Bacterial Infections

“…These results show that mesoporous silica-delivered AMPs have advantages for the treatment of bacterial infections. [73] However, the coupling of peptides to their porous materials requires the consideration of various factors. For example, the study by Atefyekta et al also found that the matching between pore size and peptides is crucial.…”

“…Reproduced with permission. [ 73 ] Copyright 2022, American Chemical Society. B) Biodistribution of AMPs in mice.…”

Recent Advances in Antimicrobial Peptide Hydrogels

“…In addition, MSNs have a large surface area and a large porous volume, which improve the ability to load therapeutic cargo [ 132 , 133 ]. In a recent study by Ma and coworkers, the objective was to design an MSNs modified with an ovotransferrin-derived AMP that could act as a mechanism to interact with the bacteria and produce antimicrobial activities against them [ 134 ]. The amphipathic AMP OVTp12 was derived from egg white ovotransferrin, which displayed excellent antimicrobial activity through the disruption of the bacterial cell membrane [ 135 ].…”