Oviduct-derived Embryo Growth Promoting Activity: A Comparison of Three Ductal Organs.

Goto, Yasuo; Noda, Yoichi; Narimoto, Kazutaka; Shiotani, Masahide; Tokura, Takashi; Umaoka, Yoh; Natsuyama, S.; Mori, Takahide; Ogawa, Kazuo

doi:10.1262/jrd.39.85

Cited by 3 publications

(1 citation statement)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Five-week-old FVB/N female mice were sacrificed as embryo donors because of their good offspring vitality and coat color (white), which was distinguishable from the pseudopregnant recipients (black). The day a vaginal plug was observed following mating was designated 0.5 dpc [9]. Two-to three-month-old F1 C57BL/6 × DBA/2 (B6D2F1) female mice were used as pseudopregnant recipients at 0.5 dpc.…”

Section: Animalsmentioning

confidence: 99%

Success of Murine Embryo Transfer Increased by a Modified Transfer Pipette

Zhang

Wang

et al. 2009

Journal of Reproduction and Development

View full text Add to dashboard Cite

Abstract. Embryos transfer is one of the most critical steps for generating genetically modified mice. Because of the limitations of the current transfer equipment and techniques, such as discharge of freshly transferred embryos and the necessity of sophisticated transfer skills, the success ratio of implantation may not well satisfy the requirements for mass production of high quality animals in the field of genetically modified mice. In this study, we describe a modified embryo transfer pipette with a syringe-like tip that can easily be applied to mouse embryo transfer. Using this improved method, we show that the procedures for mouse embryo implantation are simplified and easier to perform; moreover, the viability rate of mouse embryos is 20% higher than that achieved with conventional methods. Our modified tool and improved transfer technique are effective, time-saving and less invasive, resulting in increased success of embryo transfer.

show abstract

Section: Animalsmentioning

confidence: 99%

Success of Murine Embryo Transfer Increased by a Modified Transfer Pipette

Zhang

Wang

et al. 2009

Journal of Reproduction and Development

View full text Add to dashboard Cite

show abstract

Spatio-temporal expression of pamlin during early embryogenesis in sea urchin and importance of N-linked glycosylation for the glycoprotein function

Katow

Komazaki

1996

Roux's Arch Dev Biol

View full text Add to dashboard Cite

Expression of pamlin, a heterotrimeric primary mesenchyme cell (PMC) adhesion glycoprotein, and its role during early embryogenesis were examined using immunochemistry and microinjection of pamlin to tunicamycin-treated embryos of the sea urchin, Hemicentrotus pulcherrimus. Pamlin faintly detected in egg cortex before fertilization was strongly expressed in the hyaline layer after fertilization. The embryonic apical surface retained pamlin throughout early embryogenesis, whereas pamlin on the basal surface showed a dynamic change of spatio-temporal distribution from morula to gastrula stage. Pamlin distributed on the entire basal surface of the ectoderm before onset of invagination gradually disappeared from the presumptive archenteron during gastrulation, and then was restricted to the apical tuft region and the PMC sessile sites in early gastrulae. Tunicamycin, an inhibitor of N-glycosydically linked carbohydrate formation, inhibited PMC migration and gastrulation. Tunicamycin also inhibited the assembly of mannose moieties of 180 and 52 kDa subunits of pamlin. Pamlin microinjection to the tunicamycin-treated embryos rescued them from this morphogenetic disturbance. PMCs did not bind to pamlin isolated from the tunicamycin-treated embryos. The present study indicated that pamlin plays an essential role in PMC migration, its termination and gastrulation, and the presence of N-glycosydically linked carbohydrate moieties that contain mannose are necessary to preserve the biological function of pamlin.

show abstract

Estudo Comparativo Da Sensibilidade De Culturas Primárias De Células Epiteliais De Oviduto Bovino E Murino Ao Herpesvírus Bovino Tipo 1 (Bhv-1)

D'Angelo,

Galuppo,

Gonçalves

2002

Arq. Inst. Biol.

View full text Add to dashboard Cite

RESUMO A co-cultura de células epiteliais de oviduto bovino (BOEC) supre as necessidades de desenvolvimento dos embriões bovinos do estádio de mórula até blastocisto expandido no processo de fecundação in vitro (FIV). Este estudo avaliou a sensibilidade das células epiteliais de oviduto bovino e murino ao vírus da rinotraqueíte infecciosa bovina. As culturas primárias de células epiteliais de oviduto bovino e murino foram infectadas com o vírus da rinotraqueíte infecciosa bovina (105.6 TCID 50/mL). O efeito citopático foi observado 48 horas após a infecção, e o vírus titulado em células MDBK. A avaliação preliminar mostrou que tanto as células de oviduto bovino quanto as de oviduto murino apresentaram efeito citopático característico do vírus da rinotraqueíte infecciosa bovina, com título, respectivamente de 106.5 TCID 50/mL e 104.8 TCID 50/ mL. Este estudo mostra que as células de oviduto murino são menos susceptíveis ao vírus da rinotraqueíte infecciosa bovina, as quais podem apresentar vantagens no co-cultivo de embriões bovinos fecundados in vitro

show abstract

Oviduct-derived Embryo Growth Promoting Activity: A Comparison of Three Ductal Organs.

Cited by 3 publications

References 17 publications

Success of Murine Embryo Transfer Increased by a Modified Transfer Pipette

Success of Murine Embryo Transfer Increased by a Modified Transfer Pipette

Spatio-temporal expression of pamlin during early embryogenesis in sea urchin and importance of N-linked glycosylation for the glycoprotein function

Estudo Comparativo Da Sensibilidade De Culturas Primárias De Células Epiteliais De Oviduto Bovino E Murino Ao Herpesvírus Bovino Tipo 1 (Bhv-1)

Contact Info

Product

Resources

About