Success of Murine Embryo Transfer Increased by a Modified Transfer Pipette

Abstract

Abstract. Embryos transfer is one of the most critical steps for generating genetically modified mice. Because of the limitations of the current transfer equipment and techniques, such as discharge of freshly transferred embryos and the necessity of sophisticated transfer skills, the success ratio of implantation may not well satisfy the requirements for mass production of high quality animals in the field of genetically modified mice. In this study, we describe a modified embryo transfer pipette with a syringe-like tip that can easily be applied to mouse embryo transfer. Using this improved method, we show that the procedures for mouse embryo implantation are simplified and easier to perform; moreover, the viability rate of mouse embryos is 20% higher than that achieved with conventional methods. Our modified tool and improved transfer technique are effective, time-saving and less invasive, resulting in increased success of embryo transfer.

Decontamination of genetically modified mice strains by embryo transfer for obtaining SPF colonies in a Brazilian animal facility

“…The high skill requirements can be considered a limiting factor. Some studies provide alternatives with good results for the execution of embryo transfer using modified transfer pipettes or diversified methodologies, including the transfer of embryos in more advanced stages (Davis, 1981;Chin & Wang, 2001;Zhang et al, 2009;Sarvari et al, 2013;Cui et al, 2014). In our opinion, we considered that routine practice and appropriate training contributed to the acquisition of necessary skills for the surgical technique.…”

<b>Rederivação de linhagens de camundongos por transferência embrionária para obtenção de colônias livres de patógenos específicos (SPF)</b>

“…A técnica envolveu habilidade do executor em microcirurgia com horas de prática e treinamento. Como a habilidade em microcirurgia pode ser considerado um fator limitante, alguns trabalhos fornecem alternativas com bons resultados, para a realização da transferência embrionária com o uso de pipetas modificadas ou metodologias diversificadas, como a transferência de embriões em estágio mais avançado (DAVIS, 1981;CHIN;WANG, 2001;ZHANG et al, 2009;SARVARI et al, 2013;CUI et al, 2014). Consideramos que a prática rotineira e o treinamento adequado contribuíram para a aquisição das habilidades necessárias para a realização da técnica cirúrgica.…”