Ovicidal and larvicidal activity of extracts from medicinal-plants against Haemonchus contortus

Váradyová, Zora; Pisarčíková, Jana; Babják, Michal; Hodges, Alfred; Mravčáková, Dominika; Kišidayová, Svetlana; Königová, Alžbeta; Vadlejch, Jaroslav; Várady, Marián

doi:10.1016/j.exppara.2018.10.009

Cited by 34 publications

(38 citation statements)

References 47 publications

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“…However, the methanogenesis in vitro is not necessarily related to the density of the protozoa population (Bhatta et al, 2013). All the medicinal plants used in the present experiment have different content of kaempferol (Váradyová, Pisarčíková, Babják, et al, 2018), which was found to influence ruminal microbes (Lowry & Kennedy, 1996). Additionally, our qualitative phytochemical screening did not reveal any flavonoids in A. absinthium (Table 2).…”

Section: Discussionmentioning

confidence: 99%

“…The four traditional medicinal plants in the present study were selected based on their antiparasitic properties from our recent in vitro study (Váradyová, Pisarčíková, Babják, et al, 2018). Supplementing the diets of sheep with these medicinal plants has therefore been hypothesized to influence the microbial ecosystems in the rumen and large intestine and also to affect ruminal antioxidant capacity, fermentation patterns and the protozoan population in sheep.…”

Section: Introductionmentioning

confidence: 99%

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Effect of dry medicinal plants (wormwood, chamomile, fumitory and mallow) on in vitro ruminal antioxidant capacity and fermentation patterns of sheep

Petrič

Mravčáková

Kucková

et al. 2020

Animal Physiology Nutrition

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The objective of this study was to determine the effect of dry medicinal plants (wormwood, chamomile, fumitory and mallow) and dietary substrates containing a mix of the plants on the end products of in vitro ruminal and intestinal fermentation, rumen protozoan population and ruminal antioxidant capacity of sheep. The experiment consisted of fermentations with the four plants used individually as the sole substrate and fermentation of a mix of medicinal plants (Plants): meadow hay:barley grain (MH:B), 700/300 w/w and Plants:MH:B, 100/600/300 w/w/w. The experiment was conducted using the in vitro gas production technique (IVGPT) with 35 ml of buffered inocula and approximately 250 mg (DM basis) of substrate incubated for 24 hr at 39°C in anaerobic conditions. Quantitative analyses of the bioactive compounds by ultra‐high‐resolution mass spectrometry in Plants identified three main groups: flavonoids (22 mg/g DM), phenolic acids (15 mg/g DM) and alkaloids (3 mg/g DM). The total antioxidant capacity (TAC) of the plant extracts and rumen fluid was analysed using a ferric reducing antioxidant power assay. The values of total and individual short‐chain fatty acids, acetate:propionate ratio, pH and total gas production were significantly affected by the single plant substrates and inocula (p < .001). Apart from these parameters, the values of ammonia N, methane production and total gas produced were decreased in Plants:MH:B in comparison with MH:B (p < .001). A positive correlation was recorded between total polyphenols content and TAC of plant extracts (R2 = .778, p < .001). The counts of the total ciliate protozoan population in rumen fluid after 24 hr of fermentation were not significantly different (p > .05). Results suggest that the dietary substrate containing the medicinal plant mix possessed strong ruminal antioxidant capacity, had the potential to reduce methane emission and ammonia concentration and caused desirable changes in the gastrointestinal ecosystem.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effect of dry medicinal plants (wormwood, chamomile, fumitory and mallow) on in vitro ruminal antioxidant capacity and fermentation patterns of sheep

Petrič

Mravčáková

Kucková

et al. 2020

Animal Physiology Nutrition

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“…In traditional medicine, whole plants or mixtures of plants are used rather than isolated compounds, and therefore more research is needed on all types of interaction between plant constituents. The ultra-high-resolution mass spectrometry (UHRMS) analyses of dry medicinal plants or plant mixtures in our recent studies with H. contortus [15][16][17] identified a wide range of bioactive compounds with important pharmacological activities, mainly flavonoids, phenolic acids, diterpenes, and alkaloids. These experiments, which combined chromatographic analyses with the determination of antioxidant capacity, are helpful in identifying plants with consistent concentrations of anthelmintic and antioxidant compounds for in vitro and in vivo studies.…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, in the present study, we hypothesize that some medicinal plants from these mixtures are by themselves multicomponent mixes and can elicit effects via pharmacological activity on H. contortus infected lambs. The medicinal plants, wormwood and mallow, were chosen based on their previously described best phytotherapeutic properties and anthelmintic activity in vitro [15]. The goal is to determine the effect of dietary supplementation with wormwood and mallow on parasitological status and inflammatory parameters of lambs experimentally infected with H. contortus.…”

Section: Introductionmentioning

confidence: 99%

Anthelmintic Activity of Wormwood (Artemisia absinthium L.) and Mallow (Malva sylvestris L.) against Haemonchus contortus in Sheep

Mravčáková

Komáromyová

Babják

et al. 2020

Animals

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The objective of this study is to evaluate the effect of dry wormwood and mallow on the gastrointestinal parasite of small ruminants Haemonchus contortus. Twenty-four experimentally infected lambs were randomly divided into four groups of six animals each: unsupplemented lambs, lambs supplemented with wormwood, lambs supplemented with mallow and animals supplemented with a mix of both plants. Faecal samples from the lambs were collected on day 23, 29, 36, 43, 50, 57, 64 and 75 post-infection for quantification of the number of eggs per gram (EPG). The mix of both plants contained phenolic acids (10.7 g/kg DM) and flavonoids (5.51 g/kg DM). The nematode eggs were collected and in vitro egg hatch test was performed. The aqueous extracts of both plants exhibited strong ovicidal effect on H. contortus, with ED50 and ED99 values of 1.40 and 3.76 mg/mL and 2.17 and 5.89 mg/mL, respectively, in the in vitro tests. Despite the great individual differences between the treated lambs in eggs reduction, the mean EPG of the untreated and treated groups did not differ (p > 0.05). Our results indicate that using wormwood and mallow as dietary supplements do not have a sufficient effect on lambs infected with H. contortus.

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“…The roles of the various components of lipophilic molecule transport systems (passive diffusion, active influx, and active efflux) need to be investigated further, as well as the role and production of lipids in nematodes. This knowledge may therefore make it possible to identify new targets for anthelmintics, like other targets described in recent research in order to counter multiple resistance [8,14,46,53,57,79].…”

Section: Discussionmentioning

confidence: 96%

Effects of cholesterol content on activity of P-glycoproteins and membrane physical state, and consequences for anthelmintic resistance in the nematodeHaemonchus contortus

et al. 2020

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Eukaryote plasma membranes protect cells from chemical attack. Xenobiotics, taken up through passive diffusion, accumulate in the membranes, where they are captured by transporters, among which P-glycoproteins (Pgps). In nematodes such as Haemonchus contortus, eggshells and cuticles provide additional protective barriers against xenobiotics. Little is known about the role of these structures in the transport of chemical molecules. Pgps, members of the ABC transporter family, are present in eggshells and cuticles. Changes in the activity of these proteins have also been correlated with alterations in lipids, such as cholesterol content, in eggshells. However, the cellular mechanisms underlying these effects remain unclear. We show here that an experimental decrease in the cholesterol content of eggshells of Haemonchus contortus, with Methyl-beta-CycloDextrin (MβCD), results in an increase in membrane fluidity, favouring Pgp activity and leading to an increase in resistance to anthelmintics. This effect is modulated by the initial degree of anthelminthic resistance of the eggs. These results suggest that eggshell fluidity plays a major role in the modulation of Pgp activity. They confirm that Pgp activity is highly influenced by the local microenvironment, in particular sterols, as observed in some vertebrate models. Thus, eggshell barriers could play an active role in the transport of xenobiotics.

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Ovicidal and larvicidal activity of extracts from medicinal-plants against Haemonchus contortus

Cited by 34 publications

References 47 publications

Effect of dry medicinal plants (wormwood, chamomile, fumitory and mallow) on in vitro ruminal antioxidant capacity and fermentation patterns of sheep

Effect of dry medicinal plants (wormwood, chamomile, fumitory and mallow) on in vitro ruminal antioxidant capacity and fermentation patterns of sheep

Anthelmintic Activity of Wormwood (Artemisia absinthium L.) and Mallow (Malva sylvestris L.) against Haemonchus contortus in Sheep

Effects of cholesterol content on activity of P-glycoproteins and membrane physical state, and consequences for anthelmintic resistance in the nematodeHaemonchus contortus

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