Overview of Urea and Creatinine

Salazar, Jose H.

doi:10.1309/lm920sbnzpjrjgut

Cited by 133 publications

(101 citation statements)

References 5 publications

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“…The concentration of creatinine and urea illustrates the filtration capacity of the glomerulus, and it can be used as an indicator of renal function (Salazar, 2014). Our enzyme has no effect on the level of urea, creatinine, cholesterol and triglyceride in the blood of Sprague Dawley rats, and these results were in complete agreement with that recorded for the effect of glutaminase on rhesus monkeys and rabbits by (Hambleton et al (1980).…”

Section: Discussionsupporting

confidence: 90%

Purification, characterization and anticancer efficiency of L-glutaminase from Aspergillus flavus

Abu‐Tahon

Isaac

2019

J. Gen. Appl. Microbiol.

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The aim of this study was to purify L-glutaminase from Aspergillus flavus. The enzyme was purified 12.47-fold from a cell-free extract with a final specific activity of 613.3 U/mg and the yield was 51.11%. The molecular weight of the enzyme, as determined by SDS-PAGE, was found to be 69 kDa. The maximal activity of L-glutaminase was recorded at pH 8 and 40 o C. The highest activity was reported towards its natural substrate, L-glutamine, with an apparent Km value of 4.5 mmol and V max was 20 Uml-1. The enzyme was activated by Na + and Co +2 , while it was strongly inhibited by iodoacetate, NEM, Zn +2 and Hg +2 at 10 mM. L-glutaminase activity increased gradually with an increase of NaCl concentration up to 15%. In vivo, the median lethal dose (LD 50) was approximately 39.4 mg/kg body weight after intraperitoneal injection in Sprague Dawley rats. Also, L-glutaminase showed no observed changes in liver and kidney functions and hematological parameters on rates. Purified A. flavus L-glutaminase had neither an appreciable effect on human platelet aggregation nor hemolytic activity. In addition, MTT assay showed that the purified L-glutaminase has a high toxic effect on Hela and Hep G2 cell lines with an IC 50 value 18 and 12 µg/ml, respectively, and a moderate cytotoxic effect on HCT-116 and MCF7 cells, with an IC 50 value 44 and 58 µg/ml, respectively.

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Section: Discussionsupporting

confidence: 90%

Purification, characterization and anticancer efficiency of L-glutaminase from Aspergillus flavus

Abu‐Tahon

Isaac

2019

J. Gen. Appl. Microbiol.

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“…Основним продуктом розпаду білків є сечовина -невелика органічна молекула, що включає дві аміногрупи (NH2) і пов'язану карбамільну групу (C-O) [2,3]. На частку сечовини припадає більшість (80-90%) небілкових азотистих сполук, що переважно виводяться організмом із сечею.…”

Section: аналіз останніх досліджень і публікаційunclassified

“…Рівень сечовини у крові залежить від вмісту білка в раціоні, здатності організму катаболізувати білок і адекватної екскреції сечовини нирками. Вважається, що підвищення вмісту сечовини у крові може бути результатом згодовування раціону з високим вмістом білка або зниження рівня екскреції нирками [3]. Реакція організму здорової тварини на дію технологічного подразника залежить від його сили, походження, тривалості дії.…”

Section: мета завдання та методика дослідженьunclassified

Dynamics of urea content in blood of sows depending on peculiarities of nervous system activity

Postoi¹,

Karpovskyi²,

Данчук³

et al. 2019

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The article presents the results of studies on the dynamics of urea content in blood serum of sows depending on the type of higher nervous activity and the initial tone of the autonomic nervous system under exposure to technological stimulus. Dry sows of large white breed were used for experiments. Types of higher nervous activity in pigs were determined using the method of conditioned food reflexes. According to the studying of conditioned reflex activity 4 experimental groups were formed, 5 animals in each. The 1-st group consisted of sows with strong balanced mobile, the 2-ndstrong balanced inert, the 3-rdstrong unbalanced, and the 4-thweak types of higher nervous activity. Then in experimental animals we studied the tone of the autonomic nervous system using trygeminovagal test, by the results of which we formed 3 experimental groups (normotonics, sympathicotonics, vagotonics), 5 animals in each. Before exposure to technological stimulus and in 1, 3, 7, 14 and 28 days after its impact, blood samples were taken for biochemical studies in all experimental animals. Regrouping and moving of pigs into another building were used as a technological stimulus. In the conditions of relative rest in sows, depending on the type of autonomous regulation of the cardiovascular system, the significant differences of urea content in blood serum were not detected. Instead, in the conditions of relative rest in the representatives with weak type of higher nervous activity, lower urea content in blood serum was established than in the representatives of strong types. Due to the exposure to technological stimulus in animals of all groups, an increase in urea level in blood serum was detected. However, the degree of changes manifestation in urea content in blood serum differed in sows, depending on the type of higher nervous activity and the initial tone of autonomic nervous activity. Despite that found differences were statistically significant, they were within the normal range. Thus, the urea content in blood serum of sows is somewhat limited by the typological features of the nervous system.

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“…Kreatinin dihasilkan secara endogen di dalam tubuh dan difiltrasi di glomerolus. Adanya penurunan GFR akan menyebabkan peningkatan konsentrasi kreatinin plasma 18 . Faktor lain yang mempengaruhi kadar kreatinin serum di antaranya faktor diit, aktivitas fisik dan perombakan masa otot 17 .…”

Section: Pembahasanunclassified

“…Faktor lain yang mempengaruhi kadar kreatinin serum di antaranya faktor diit, aktivitas fisik dan perombakan masa otot 17 . Selain akibat penurunan fungsi ginjal, peningkatan kadar ureum serum dapat dipengaruhi diit tinggi protein dan keadaan dehidrasi 18 .…”

Section: Pembahasanunclassified

Left Ventricular Hypertrophy dan Fungsi Ginjal pada Penderita Hipertensi

2018

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Introduction: Chronic hypertension can cause complication such as left ventricular hypertrophy (LVH). LVH can lead renal artery vasoconstriction, impaire blood perfusion to glomerulus and change glomerulus filtration coeficient. The aim of this study was to compare kidney function between hypertensive patient with LVH and without LVH Methods: This was a case control study that conducted at Dr. Moewardi General Hospital Surakarta. All subjects has been diagnosed as hypertension more than 5 years. The diagnosis of LVH was based on electrocardiography (ECG) result. Kidney function was assessed based on serum ureum, creatinin, and uric acid levels. Results: The subject of this study was 26 hypertension patients with LVH and 13 hypertension patients without LVH. Serum ureum level in patients with LVH was higher than hypertension patients without LVH(24.92 ± 7.99 Vs 32.33±9.91, p= 0.004). Serum uric acid level in hypertension patients with LVH was higher compare to hypertension patients without LVH as well (5.26 ± 1.15 Vs 6.98 ± 1.83, p= 0.029). Whereas serum creatinine levels were not significantly different between two groups of subjects. Sulastomo et.al., Left Ventricular Hypertrophy dan Fungsi Ginjal pada Penderita Hipertensi Conclusion: : Serum ureum and uric acid levels in hypertensive subjects with LVH are higher than subjects without LVH. There is no difference between serum creatinin level in hypertensive subject with LVH and without LVH.

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Overview of Urea and Creatinine

Cited by 133 publications

References 5 publications

Purification, characterization and anticancer efficiency of L-glutaminase from <i>Aspergillus flavus</i>

Purification, characterization and anticancer efficiency of L-glutaminase from <i>Aspergillus flavus</i>

Dynamics of urea content in blood of sows depending on peculiarities of nervous system activity

Left Ventricular Hypertrophy dan Fungsi Ginjal pada Penderita Hipertensi

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