Overripe eggs and postovulatory corpora lutea in the threespine stickleback, Gasterosteus aculeatus L., form trachurus

Lam, T.J.; Nagahama, Yoshitaka; Chan, Kenny Chi Wai; Hoar, William S.

doi:10.1139/z78-273

Cited by 54 publications

(50 citation statements)

References 7 publications

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“…De acordo com Lam et al (1978), ovócitos que atingiram diâmetros maiores que aqueles da ovulação, podem ou não ser eliminados e são denominados de "overripe"; quando eliminados, não são fertilizáveis, pois já entraram em processo de degeneração ou atresia. McEvoy (1984) confirma este fato, declarando que esses ovócitos são inviáveis.…”

Section: Resultsunclassified

Reprodução induzida da piabanha, Brycon insignis (Steindachner, 1876), mantida em cativeiro

et al. 2002

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RESUMO -Estudos de reprodução induzida da piabanha, Brycon insignis, foram realizados em janeiro-fevereiro/96 e fevereiro/97, utilizando-se exemplares de 3 a 5 anos de idade, mantidos em cativeiro na Estação Experimental da Seção de Hidrobiologia e Aqüicultura, pertencente à CESP (Companhia Energética de São Paulo), localizada na cidade de Paraibuna, SP. As fêmeas selecionadas para indução da reprodução apresentaram ventre abaulado, papila genital saliente e avermelhada e 60% dos ovócitos com núcleo em posição excêntrica. Trinta e três fêmeas foram induzidas à reprodução com dose única de hCG de 5 UI/g (gonadotropina coriônica humana); 17 receberam duas aplicações de extrato bruto de hipófise de carpa, de 0,5 e 5,0 mg/kg, com intervalo de 10 horas entre as aplicações; 18 receberam 0,5 mg/kg de extrato bruto de hipófise de carpa e, após 10 horas, 5 UI/g de hCG; 5 fêmeas receberam dose única de solução salina a 0,9%. Cerca de 10 horas após a última aplicação hormonal, 43 fêmeas responderam positivamente aos tratamentos hormonais liberando óvulos por extrusão. A distribuição de freqüência porcentual dos diâmetros dos ovócitos da amostra inicial apresentou tendência unimodal, com moda em 1250 µm. Entre os hormônios testados, o mais eficiente foi o hCG, em dose única, e o menos eficiente, o de hipófise de carpa, com aplicação de duas doses. Os machos receberam dose única de 3 UI de hCG/g. Cada fêmea liberou, em média, 24.690 óvulos, que, após a fertilização e hidratação, se apresentaram esféricos, demersais e não adesivos, com diâmetro médio de 3785 a 3900 µm, grande espaço perivitelínico e córion resistente. A taxa de fertilização oscilou entre 20 e 96% e a de eclosão, entre 0 e 85%. A temperatura da água foi de 26 o C ± 1 o C. Palavras-chave: Brycon insignis, peixe de água doce, piabanha, reprodução induzida Induced Spawning of "Piabanha" (Brycon insignis Steindachner, 1876) in CaptivityABSTRACT -Studies of induced spawning of piabanha, Brycon insignis , were developed from January to February/96 and February/97, with 3 to 5 years old individuals maintained in captivity. The selected females presented swollen abdomen and red genital papilla, 60% of oocytes with eccentric germinal vesicle. Thirty-three females were induced to spawning through a single dose of 5 IU/ g of hCG (human Chorionic Gonadotropin); 17 females received two application: 0.5 and 5 mg/kg of crude carp pituitary extract in an interval of 10 hours; 18 received one application of 0.5 mg/kg of crude carp pituitary extract and, after 10 hours, 5 IU/g of hCG; and 5 females received a single dose of 0.9% saline solution. About 10 hours after the last hormonal application, 43 females responded positively to the hormonal treatment releasing eggs by extrusion. The percent frequency distribution of the oocytes diameter in the initial sample presented unimodal tendency, with mode in 1250 µm. Considering the different hormones utilized, the most efficient was hCG in a single dose and the less efficient was the hormone of carp pituitary applicated in two doses...

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Section: Resultsunclassified

Reprodução induzida da piabanha, Brycon insignis (Steindachner, 1876), mantida em cativeiro

et al. 2002

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“…The short-lived nature of postovulatory follicles in goldfish was also reported by Nagahama et al (1976) based on ultrastructural studies. An association between overripening and degenerative changes in postovulatory follicles was first noted by Lam et al (1978) in the threespined stickleback, Gasterosteus aculeatus. Lam et al (1978Lam et al ( , 1979 postulated that POFs while active secrete steroids which prevent the overripening of ovulated eggs in the ovarian cavity.…”

Section: Discussionmentioning

confidence: 97%

“…However, the underlying causes are still not understood. Lam et al (1978Lam et al ( , 1979 have shown a relationship between overripening and the degeneration of postovulatory follicles (POFs) in the threespined stickleback (Gasterosteus acculeatus), and postulated that steroids (e.g., progesterone) secreted by the POFs stimulate the secretion of ovarian fluid which helps maintain the viability of ovulated eggs in the ovarian cavity. In goldfish, Nagahama et al (1976) have shown that POFs degenerate rapidly (within 30h) based on ultrastructural and histochemical evidence.…”

Section: Introductionmentioning

confidence: 99%

“…This bears a relationship to the time course of overripening obtained in this study. Therefore, to further examine the hypothesis of Lam et al (1978Lam et al ( , 1979, changes in the levels of serum and ovarian fluid steroid hormones during overripening, together with the histology of the POFs, were studied in goldfish. In vivo and in vitro experiments were also carried out to test the possible involvement of certain steroids in the process of overripening.…”

Section: Introductionmentioning

confidence: 99%

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Overripening of ovulated eggs in goldfish, Carassius auratus: II. Possible involvement of postovulatory follicles and steroids

Formacion

Venkatesh

Tan

et al. 1995

Fish Physiol Biochem

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Changes in steroid hormone levels in the serum and ovarian fluid during overripening were studied in goldfish. Ovulated eggs retained in the ovarian cavity became overripe at around 12h after ovulation based on loss of fertilizability, with advanced degeneration by 24h. Blood and ovarian fluid were taken at 0, 3, 6, 12, 18 and 24h after ovulation. Both serum and ovarian fluid progesterone (P) showed a highly significant decline by 18h with a further decline by 24h; P levels were higher in the ovarian fluid. Serum 17α,20β-dihydroxy-4-pregnen-3-one(17,20β-P) levels showed a progressive and more rapid decline, decreasing significantly by 12h with further decreases by 18h and 24h. Serum testosterone (T) levels increased significantly at 3h and remained high till 18h, thereafter they declined to the 0h level. No significant changes in estradiol-17β (E2) levels were observed in the serum, except for a significant difference between 6 and 24h. There were no significant changes in ovarian fluid E2, T or 17,20β-P levels.The postovulatory follicles (POFs) showed degenerative changes which corresponded to the decline in P and 17,20β-P. The hypothesis that overripening may be associated with declining levels of P or 17,20β-P was tested in vivo by immersing just ovulated females (0h) in P or 17,20β-P solutions for 12h, and in vitro by immersing just-ovulated eggs (0h) in ovarian fluid with an anti-serum against P (Anti-P). In the former, P at 0.05 ppm significantly enhanced the fertilization rate of ovulated eggs stripped from the females at 12h while 17,20β-P did not produce a significant effect at the tested concentrations (0.025 and 0.05 ppm) on the fertilization rate. In the latter, anti-P significantly lowered the fertilization rate of 0h ovulated eggs after 6h incubation. The evidence suggests a role of P in the maintenance of viability of ovulated eggs.

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“…Linhart and Billard 1995). The reduction in permeability of essential ions (Lam et al 1978), error in the chromosomal distribution (Saito et al 1993), and chromosomal aberration (Yamazaki et al 1989) are some of the causes reported during aging phenomenon responsible for abnormal larval production. As few as 4%-5% of deformed larvae were observed when the female was injected with 4000 IU hCG and stripped at 14-17 h post-injection.…”

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confidence: 99%

Observation on larval deformity during induced spawning of walking catfish, Clarias batrachus (Actinopterygii: Siluriformes: Clariidae), at different combinations of human chorionic gonadotropin dose and latency period

Sahoo¹,

Giri²,

Chandra³

et al. 2009

Acta Icth et Piscat

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The walking catfish, Clarias batrachus (L.), is a potential species for aquaculture. Its production has been documented at few occasions (Areerat 1987, Thakur andDas 1986). Availability of quality seed is one of the important pre-requisites for successful culture of any species. So induced spawning is always advisable for getting optimum quantity and quality of the stocking material.The breeding performance and egg quality of C. batrachus has been reported by the use of different dose of inducing agents with latency combinations in several occasions (Zonneveld et al. 1988, Sahoo et al. 2005, 2007. The deformed larvae like bend trunk and tail, acephalic and tunicate larvae are often encountered along with the normal larvae during breeding operations in carp (Rath et al. 1995) and catfish (Sahoo et al. 2004). The quantity of deformed larvae in Silurus glanis was observed as high as 50% during hatchery production (Linhart and Billard 1995). The deformity in larvae is originated due to poor egg quality, impact of environment or due to fertilization of over-ripped eggs. The over-ripening of eggs occurs due to delayed in stripping of ovulated eggs. This condition is sometimes happened while working in the hatchery condition. The increase in number of deformed larvae affects the production, quality of larvae and the profitability of a hatchery. No information is available on the pattern of deformed larval production while using human chorionic gonadotropin (hCG) as an inducing agent during induced breeding of C. batrachus. Hence the present study is aimed to communicate the pattern of deformity among the hatchlings during its induced spawning at different dose of hCG and latency period combinations.C. batrachus broods were raised in earthen ponds (0.01 ha) at the Institute and were fed with pelleted feed containing 30% crude protein at 2% of their body weight, daily. The female broods of 120-130 g weight range were selected for induced breeding during July-August. The female broods were selected as suggested earlier (Sahoo et al. 2005). The hCG was purchased and reconstituted in 1 mL of solvent provided with the pack. That was further diluted with normal saline solution to get required concentrations of injectable hCG. The groups of females were injected with 1000, 2000, 3000, 4000, and 5000 IU hCG per kg body weight, and stripped after five latency periods, 11, 14, 17, 20, and 23 h in 25 (5 × 5) different combinations. Each group of fish comprised of five females and, was considered for each dose level and latency period combination.The testes were removed from three male fish, incised and squashed in normal saline solution (0.89% NaCl) to get sperm suspension. A drop of sperm suspension was ACTA ICHTHYOLOGICA ET PISCATORIA (2009) Abstract. Five combinations of human chorionic gonadotropin (hCG) dose and five latency periods were evaluated to observe the pattern of deformed larvae among the hatchlings in walking catfish, Clarias batrachus (L.). A higher percentage (7%-12%) of deformed larvae were observed at 1000, 20...

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Overripe eggs and postovulatory corpora lutea in the threespine stickleback, Gasterosteus aculeatus L., form trachurus

Cited by 54 publications

References 7 publications

Reprodução induzida da piabanha, Brycon insignis (Steindachner, 1876), mantida em cativeiro

Reprodução induzida da piabanha, Brycon insignis (Steindachner, 1876), mantida em cativeiro

Overripening of ovulated eggs in goldfish, Carassius auratus: II. Possible involvement of postovulatory follicles and steroids

Observation on larval deformity during induced spawning of walking catfish, <I>Clarias batrachus</I> (Actinopterygii: Siluriformes: Clariidae), at different combinations of human chorionic gonadotropin dose and latency period

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