Overpressured-layer chromatography

Tyihák, E.; Móricz, Ágnes M.; Mincsovics, Emil

doi:10.1016/b978-0-12-420161-3.00002-2

Cited by 6 publications

(2 citation statements)

References 84 publications

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“…In comparison, the online sample application seems to pose some technical difficulties and results in pronounced dispersion of solute zones/ peaks. [1][2][3][9][10][11][12]17 The other approach to force the mobile phase flow through the adsorbent layer is the use of EOF. It has some advantages, as it does not generate backpressure, as in the case of the hydrodynamic flow.…”

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High Performance (High Pressure) Layer Electrochromatography Separation Technique: Equipment and Preliminary Results

Gwarda

Dzido

2022

Anal. Chem.

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We present a new prototype device and propose a new analytical technique: high performance (high pressure) layer electrochromatography (HPLEC). The equipment provides a combination of overpressured layer chromatography (OPLC) and pressurized planar electrochromatography (PPEC), yet it still enables researchers to perform each of these analyses separately. In comparison to PPEC, HPLEC provides hydrodynamic flow of the mobile phase, irrespective of the voltage used and the mobile phase composition. The advantages of HPLEC over OPLC include the possibility of the use of the electrophoretic effect to influence the selectivity of separation and the use of the electroosmotic effect to facilitate the mobile phase flow in order to decrease backpressure and increase the flow velocity. Many operational parameters can be freely adjusted and optimized independently. The equipment is fully automated and can work in various separation/operational modes, including combinations of online/offline sample application and detection. We present preliminary results of simultaneous, fully online, multichannel HPLEC separation of analgesic drugs (including acetaminophen, ibuprofen, and tramadol) as an example of increasing analysis throughput.

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High Performance (High Pressure) Layer Electrochromatography Separation Technique: Equipment and Preliminary Results

Gwarda

Dzido

2022

Anal. Chem.

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“…Improvements have also been made in the use of higher quality adsorbents along with the overpressure mobile phase. In this regard, reverse phase TLC and high-performance TLC are two examples [ 11 ]. Another is separating dyes with circular TLC [ 12 ].…”

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Separation of Amino Acids, Dyes, and Pigments Using Novel Pressurized Circular TLC Assembly for Secure Medical Imaging Applications

Dar

Ahmad

Samin

et al. 2023

International Journal of Analytical Chemistry

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A novel pressurized flow system for circular thin-layer chromatography (PC-TLC) has been successfully established and employed for the separation of amino acids, dyes, and pigments for safe medical imaging applications. In this system, the mobile phase is applied to a regular TLC plate through the tube and needle of an intravenous infusion set. The needle was fused in a hole underneath the center of the plate, while the second side end of the tube was connected to a microburette containing the solvent. This new assembly proved itself better in terms of separation time (within 5 minutes) and controlled flow of the solvent and horizontal movement of analyte components over chromatograms with better separation and Rf values (glutamine: 0.26, valine: 0.44, phenylalanine: 0.60, chlorophyll a: 0.52, chlorophyll b: 0.43, xanthophyll: 0.18, carotenoid: 0.97, and pheophytin: 0.60) when a number of samples of amino acids, dyes, and pigments were separated by the developed apparatus and the conventional TLC procedure. The developed method was found distinctly rapid, precise, and eco-friendly (less solvent consuming) as compared to traditional ascending TLC.

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BioArena system for studying key molecules as well as ingredients in biological samples

Tyihák

Móricz

2016

Forced-Flow Layer Chromatography

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Overpressured-layer chromatography

Cited by 6 publications

References 84 publications

High Performance (High Pressure) Layer Electrochromatography Separation Technique: Equipment and Preliminary Results

High Performance (High Pressure) Layer Electrochromatography Separation Technique: Equipment and Preliminary Results

Separation of Amino Acids, Dyes, and Pigments Using Novel Pressurized Circular TLC Assembly for Secure Medical Imaging Applications

BioArena system for studying key molecules as well as ingredients in biological samples

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