Overlapping and divergent localization of Frem1 and Fras1 and its functional implications during mouse embryonic development

Petrou, Petros; Chiotaki, Rena; Dalezios, Yannis; Chalepakis, Georges

doi:10.1016/j.yexcr.2006.12.008

Cited by 42 publications

(52 citation statements)

References 28 publications

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“…Our previous studies of mouse organogenesis suggested that Frem1 is expressed in a complementary pattern to Fras1 and Frem2 in regions of epithelial-mesenchymal interaction (Smyth et al, 2004), although recent studies indicate that Frem1 protein is detected in epithelial and distinct cell types in certain contexts (Petrou et al, 2007a). In the fin fold, a region of extensive matrix/basement membrane synthesis, this pattern of divergent and coexpression is also apparent and we propose the teleost fin fold as a model system in which to study these interactions.…”

Section: Discussionmentioning

confidence: 95%

“…In the majority of developmental contexts Fras1 and Frem2 are expressed in the epidermis whereas Frem1 is expressed in the underlying mesenchyme or dermis (Smyth et al, 2004), although this is often tissue specific and for the most part the proteins all localize to the basement membrane. Intracellular epidermal Frem1 is noted in some contexts (Chiotaki et al, 2007;Petrou et al, 2007a). These observations, along with the domain structure of the proteins, led us to speculate that the proteins interact directly to mediate epidermal adhesion (Smyth et al, 2004).…”

Section: Introductionmentioning

confidence: 95%

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Expression of the fras1/frem gene family during zebrafish development and fin morphogenesis

Gautier

Naranjo-Golborne

Taylor

et al. 2008

Developmental Dynamics

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Mouse studies have highlighted the requirement of the extracellular matrix Fras and Frem proteins for embryonic epidermal adhesion. Mutations of the genes encoding some of these proteins underlie the blebs mouse mutants, whereas mutations in human FRAS1 and FREM2 cause Fraser syndrome, a congenital disorder characterized by embryonic blistering and renal defects. We have cloned the zebrafish homologues of these genes and characterized their evolutionary diversification and expression during development. The fish gene complement includes fras1, frem1a, frem1b, frem2a, frem2b, and frem3, which display complex overlapping and complementary expression patterns in developing tissues including the pharyngeal arches, hypochord, musculature, and otic vesicle. Expression during fin development delineates distinct populations of epidermal cells which have previously only been described at a morphological level. We detect relatively little gene expression in epidermis or pronephros, suggesting that the essential role of these proteins in mediating their development in humans and mice is recently evolved. Developmental Dynamics 237:3295-3304, 2008.

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Section: Discussionmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 95%

Expression of the fras1/frem gene family during zebrafish development and fin morphogenesis

Gautier

Naranjo-Golborne

Taylor

et al. 2008

Developmental Dynamics

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“…Concerning the intracellular localization of Frem1, previous studies on mouse embryos also showed Frem1 to be localized, in distinct epidermal cell types, such as the peridermal layer, at around E16.5 (Petrou et al 2007a). Since, periderm cells are known to participate in short-term epithelial fusions like embryonic eyelid closure, this distinct feature of Frem1 expression was correlated with "eyes open at birth phenotype", a unique characteristic of Frem1 mouse mutants, which are born with open eyes (Petrou et al 2007a).…”

Section: Discussionmentioning

confidence: 89%

“…Since, periderm cells are known to participate in short-term epithelial fusions like embryonic eyelid closure, this distinct feature of Frem1 expression was correlated with "eyes open at birth phenotype", a unique characteristic of Frem1 mouse mutants, which are born with open eyes (Petrou et al 2007a). Regarding the intracellular localization of Frem1 in adult mouse tail skin at the site of basal keratinocytes, given that there is no obvious phenotype involving these cells it is diYcult to contemplate upon a certain function.…”

Section: Discussionmentioning

confidence: 99%

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Differential localization profile of Fras1/Frem proteins in epithelial basement membranes of newborn and adult mice

Pavlakis

Makrygiannis

Chiotaki

et al. 2008

Histochem Cell Biol

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The Fras1/Frem gene family encodes for structurally similar proteins of the extracellular matrix, functionally correlated with embryonic dermal-epidermal adhesion as deduced from the appearance of sub-epidermal blisters in mouse mutants compromising the function of Fras1, Frem1 and Frem2 proteins. Mutations in the human counterparts FRAS1 and FREM2 have been detected in patients suffering from Fraser syndrome. So far, Fras1/Frem proteins have been shown to be strictly colocalized in the sublamina densa of mouse epithelial basement membranes during development. Here, we focused on the characterization of the localization pattern of the aforementioned proteins, in various parts of the adult mouse skin as well as a range of organs and tissues. Frem3 was present in a broad range of epithelial basement membranes where Fras1, Frem1 and Frem2 were missing. The localization profile of Frem3 coincided with that of collagen VII in all skin basement membranes but differed in that Frem3 was additionally found in the basement membrane of several internal epithelia, where collagen VII was absent. Fras1 and Frem2 were colocalized with Frem3 in the basement membrane of certain skin parts, underlying the thin-layer, of rapidly proliferating keratinocytes, whereas Frem1 was detected only in the basement membrane of the tail. The localization pattern of Fras1 and Frem2 was indistinguishable, while both proteins along with Frem3 could be detected even in the absence of Frem1.

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Basement membranes in development and disease

Wiradjaja

DiTommaso

Smyth

2010

Birth Defects Research Pt C

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Basement membranes (BMs) are specializations of the extracellular matrix that act as key mediators of development and disease. Their sheet like protein matrices typically serve to separate epithelial or endothelial cell layers from underlying mesenchymal tissues, providing both a biophysical support to overlying tissue as well as a hub to promote and regulate cell-cell and cell-protein interactions. In the latter context, the BM is increasingly being recognized as a mediator of growth factor interactions during development. In this review, we discuss recent findings regarding the structure of the BM and its roles in mediating the normal development of the embryo, and we examine congenital diseases affecting the BM which impact embryonic development and health in later life.

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Overlapping and divergent localization of Frem1 and Fras1 and its functional implications during mouse embryonic development

Cited by 42 publications

References 28 publications

Expression of the fras1/frem gene family during zebrafish development and fin morphogenesis

Expression of the fras1/frem gene family during zebrafish development and fin morphogenesis

Differential localization profile of Fras1/Frem proteins in epithelial basement membranes of newborn and adult mice

Basement membranes in development and disease

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