Overexpression of wheat alternative oxidase gene Waox1a alters respiration capacity and response to reactive oxygen species under low temperature in transgenic Arabidopsis

Sugie, Atsushi; Naydenov, Nayden G.; Mizuno, Nobuyuki; Nakamura, Chiharu; Takumi, Shigeo

doi:10.1266/ggs.81.349

Cited by 72 publications

(44 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…3B). A similar increase of the alternative pathway activity occurred under abiotic stress conditions such as low temperature (Purvis and Shewfelt, 1993;Vanlerberghe et al, 2002;Sugie et al, 2006).…”

Section: Respiration Capacities In the Necrosis Linesmentioning

confidence: 59%

“…We previously isolated two non-homoeologous genes, Waox1a and Waox1c, encoding the AOX proteins from common wheat (Takumi et al, 2002). WAOX1a alleviates oxidative stress when the cytochrome pathway of respiration is inhibited under low temperature stress conditions (Sugie et al, 2006). The aim of the present study was to clarify the AOX function in necrosis.…”

Section: Introductionmentioning

confidence: 95%

See 1 more Smart Citation

Alteration of respiration capacity and transcript accumulation level of alternative oxidase genes in necrosis lines of common wheat

2007

Self Cite

View full text Add to dashboard Cite

Mitochondrial alternative oxidase (AOX) is the terminal oxidase responsible for cyanide-insensitive and salicylhydroxamic acid-sensitive respiration in plants. AOX is a key enzyme of the alternative respiration pathway. To study the effects of necrotic cell death on the mitochondrial function, production of reactive oxygen species (ROS), respiration capacities and accumulation patterns of mitochondriatargeted protein-encoding gene transcripts were compared between wild-type, lesion-mimic mutant and hybrid necrosis wheat plants. Around cells with the necrosis symptom, ROS accumulated abundantly in the intercellular spaces. The ratio of the alternative pathway to the cytochrome pathway was markedly enhanced in the necrotic leaves. Transcripts of a wheat AOX gene, Waox1a, were more abundant in a novel lesion-mimic mutant of common wheat than in the wildtype plants. An increased level of the Waox1a transcripts was also observed in hybrid plants containing Ne1 and Ne2 genes. These results indicated that an increase of the wheat AOX transcript level resulted in enhancement of respiration capacity of the alternative pathway in the necrotic cells.

show abstract

Section: Respiration Capacities In the Necrosis Linesmentioning

confidence: 59%

Section: Introductionmentioning

confidence: 95%

Alteration of respiration capacity and transcript accumulation level of alternative oxidase genes in necrosis lines of common wheat

2007

Self Cite

View full text Add to dashboard Cite

show abstract

“…It was observed that Arabidopsis plants expressing antisense AOX1a showed greater susceptibility to drought [12], low temperature [13], and high light [6] compared with wild-type plants. Furthermore, Arabidopsis plants constitutively overexpressing AOX1a gene had greater tolerance to high salt and low temperature stress than wild-type plants [14,15].…”

Section: Expression Of Aox Genes Under Abiotic Stressesmentioning

confidence: 98%

Expression and signal regulation of the alternative oxidase genes under abiotic stresses

Hong¹,

Guan²,

Sun³

et al. 2013

ABBS

View full text Add to dashboard Cite

Plants in their natural environment frequently face various abiotic stresses, such as drought, high salinity, and chilling. Plant mitochondria contain an alternative oxidase (AOX), which is encoded by a small family of nuclear genes. AOX genes have been shown to be highly responsive to abiotic stresses. Using transgenic plants with varying levels of AOX expression, it has been confirmed that AOX genes are important for abiotic stress tolerance. Although the roles of AOX under abiotic stresses have been extensively studied and there are several excellent reviews on this topic, the differential expression patterns of the AOX gene family members and the signal regulation of AOX gene(s) under abiotic stresses have not been extensively summarized. Here, we review and discuss the current progress of these two important issues.

show abstract

“…Notable ones include prevention of overreduction of mitochondrial electron transport chain under conditions when growth is restricted such as low temperature (Purvis and Shewfelt, 1993), drought (Collier and Commins, 1992), high salinity (Hilal et al, 1998) and low supply of minerals (Parsons et al, 1999). AOX may also function in the maintenance of constant energy charge and in scavenging reactive oxygen species in mitochondria during growth under variable environment conditions (Moore et al, 2002) as well as in apoptosis and genetic necrosis (Vanlerberghe et al, 2002;Marechal and Baldan, 2002;Mizuno et al, 2005;Sugie et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Expression profiles of respiratory components associated with mitochondrial biogenesis during germination and seedling growth under normal and restricted conditions in wheat

et al. 2008

Self Cite

View full text Add to dashboard Cite

Germination of plant embryo is a dynamic phase-changing process that is driven by a rapid increase in mitochondrial respiration. We studied the development of respiratory electron transport pathways and the profiles of their transcript and protein components during this critical period using wheat embryos. Oxygen consumption through both the cytochrome and alternative pathways increased rapidly upon imbibition. Quantitative RT-PCR analysis using specific primers and western blot analysis using specific antibodies suggested that this respiratory burst was supported both by the stored mRNA and protein components and ones synthesized de novo at least in the cytochrome pathway. Dry embryos also contained transcript and protein of alternative oxidase (AOX), but their levels remained constant during the studied period. By contrast, the alternative pathway capacity showed a marked increase when the cytochrome pathway was inhibited by antimycin A and this increase was associated with increased levels of AOX transcript and protein. Our results suggest that mitochondrial biogenesis is accompanied by sequential and differential gene expression and protein accumulation, and that AOX allows the complex I to continue to conserve energy thus to support embryo germination and initial seedling growth in wheat when the cytochrome pathway is restricted.

show abstract

Overexpression of wheat alternative oxidase gene Waox1a alters respiration capacity and response to reactive oxygen species under low temperature in transgenic Arabidopsis

Cited by 72 publications

References 24 publications

Alteration of respiration capacity and transcript accumulation level of alternative oxidase genes in necrosis lines of common wheat

Alteration of respiration capacity and transcript accumulation level of alternative oxidase genes in necrosis lines of common wheat

Expression and signal regulation of the alternative oxidase genes under abiotic stresses

Expression profiles of respiratory components associated with mitochondrial biogenesis during germination and seedling growth under normal and restricted conditions in wheat

Contact Info

Product

Resources

About