Overexpression of the Wheat (Triticum aestivum L.) TaPEPKR2 Gene Enhances Heat and Dehydration Tolerance in Both Wheat and Arabidopsis

Zang, Xinshan; Geng, Xianguo; He, Kexiang; Wang, Fei; Tian, Xiaohong; Xin, Mingming; Yao, Yingyin; Hu, Zhaorong; Ni, Zhongfu; Sun, Qixin; Peng, Huiru

doi:10.3389/fpls.2018.01710

Cited by 37 publications

(25 citation statements)

References 19 publications

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“…Previously, overexpressed tobacco plants confirmed resistance to high temperature and oxidative stress exhibiting higher physiological indicators than WT plants Zang et al, 2018) and higher survival rate and root length was observed under high temperature and NaCl stress . CaChiVI2 overexpression showed moderate heat and drought stress symptoms and increased the resistance of Arabidopsis plants (Figures 9D, 10C).…”

Section: Discussionmentioning

confidence: 95%

The CaChiVI2 Gene of Capsicum annuum L. Confers Resistance Against Heat Stress and Infection of Phytophthora capsici

et al. 2020

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Extreme environmental conditions seriously affect crop growth and development, resulting in substantial reduction in yield and quality. However, chitin-binding proteins (CBP) family member CaChiVI2 plays a crucial role in eliminating the impact of adverse environmental conditions, such as cold and salt stress. Here, for the first time it was discovered that CaChiVI2 (Capana08g001237) gene of pepper (Capsicum annuum L.) had a role in resistance to heat stress and physiological processes. The full-length open-reading frame (ORF) of CaChiVI2 (606-bp, encoding 201-amino acids), was cloned into TRV2:CaChiVI2 vector for silencing. The CaChiVI2 gene carries heat shock elements (HSE, AAAAAATTTC) in the upstream region, and thereby shows sensitivity to heat stress at the transcriptional level. The silencing effect of CaChiVI2 in pepper resulted in increased susceptibility to heat and Phytophthora capsici infection. This was evident from the severe symptoms on leaves, the increase in superoxide (O 2 −) and hydrogen peroxide (H 2 O 2) accumulation, higher malondialdehyde (MDA), relative electrolyte leakage (REL) and lower proline contents compared with control plants. Furthermore, the transcript level of other resistance responsive genes was also altered. In addition, the CaChiIV2-overexpression in Arabidopsis thaliana showed mild heat and drought stress symptoms and increased transcript level of a defense-related gene (AtHSA32), indicating its role in the co-regulation network of the plant. The CaChiVI2overexpressed plants also showed a decrease in MDA contents and an increase in antioxidant enzyme activity and proline accumulation. In conclusion, the results suggest that CaChiVI2 gene plays a decisive role in heat and drought stress tolerance, as well as, provides resistance against P. capsici by reducing the accumulation of reactive oxygen species (ROS) and modulating the expression of defense-related genes. The outcomes obtained here suggest that further studies should be conducted on plants adaptation mechanisms in variable environments.

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Section: Discussionmentioning

confidence: 95%

The CaChiVI2 Gene of Capsicum annuum L. Confers Resistance Against Heat Stress and Infection of Phytophthora capsici

et al. 2020

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“…Phosphoenolpyruvate carboxylase kinase-related kinase gene ( TaPEPKR2 ) is a highly conserved serine-threonine protein kinase gene and is reported to be induced by heat and drought. Transgenic wheat lines transformed with the TaPEPKR2 gene exhibited enhanced heat and dehydration stress tolerance and higher total root lengths in the presence of 10% PEG than wild type [89].…”

Section: Protein Kinasementioning

confidence: 99%

Development of Drought-Tolerant Transgenic Wheat: Achievements and Limitations

Khan

Anwar

et al. 2019

IJMS

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Crop yield improvement is necessary to keep pace with increasing demand for food. Due to climatic variability, the incidence of drought stress at crop growth stages is becoming a major hindering factor to yield improvement. New techniques are required to increase drought tolerance along with improved yield. Genetic modification for increasing drought tolerance is highly desirable, and genetic engineering for drought tolerance requires the expression of certain stress-related genes. Genes have been identified which confer drought tolerance and improve plant growth and survival in transgenic wheat. However, less research has been conducted for the development of transgenic wheat as compared to rice, maize, and other staple food. Furthermore, enhanced tolerance to drought without any yield penalty is a major task of genetic engineering. In this review, we have focused on the progress in the development of transgenic wheat cultivars for improving drought tolerance and discussed the physiological mechanisms and testing of their tolerance in response to inserted genes under control or field conditions.

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“…In addition, many genes responsible for heat stress tolerance have been identified and validated in model plants and also in major food crops that can be introduced to heat susceptible cultivars or their expression levels can be increased by generating their stable overexpression lines. For instance, the overexpression TaPEPKR2 gene enhanced heat stress tolerance in wheat and Arabidopsis plants 96 .…”

Section: Approaches To Enhance Heat Tolerancementioning

confidence: 99%

Functional genomic approaches to improve crop plant heat stress tolerance

et al. 2019

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Heat stress as a yield limiting issue has become a major threat for food security as global warming progresses. Being sessile, plants cannot avoid heat stress. They respond to heat stress by activating complex molecular networks, such as signal transduction, metabolite production and expressions of heat stress-associated genes. Some plants have developed an intricate signalling network to respond and adapt it. Heat stress tolerance is a polygenic trait, which is regulated by various genes, transcriptional factors, proteins and hormones. Therefore, to improve heat stress tolerance, a sound knowledge of various mechanisms involved in the response to heat stress is required. The classical breeding methods employed to enhance heat stress tolerance has had limited success. In this era of genomics, next generation sequencing techniques, availability of genome sequences and advanced biotechnological tools open several windows of opportunities to improve heat stress tolerance in crop plants. This review discusses the potential of various functional genomic approaches, such as genome wide association studies, microarray, and suppression subtractive hybridization, in the process of discovering novel genes related to heat stress, and their functional validation using both reverse and forward genetic approaches. This review also discusses how these functionally validated genes can be used to improve heat stress tolerance through plant breeding, transgenics and genome editing approaches.

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Overexpression of the Wheat (Triticum aestivum L.) TaPEPKR2 Gene Enhances Heat and Dehydration Tolerance in Both Wheat and Arabidopsis

Cited by 37 publications

References 19 publications

The CaChiVI2 Gene of Capsicum annuum L. Confers Resistance Against Heat Stress and Infection of Phytophthora capsici

The CaChiVI2 Gene of Capsicum annuum L. Confers Resistance Against Heat Stress and Infection of Phytophthora capsici

Development of Drought-Tolerant Transgenic Wheat: Achievements and Limitations

Functional genomic approaches to improve crop plant heat stress tolerance

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