Development of Drought-Tolerant Transgenic Wheat: Achievements and Limitations

Khan, Shahbaz; Anwar, Syamsul; Yu, Shuxun; Sun, Min; Yang, Zhenping; Gao, Zhiqiang

doi:10.3390/ijms20133350

Cited by 80 publications

(47 citation statements)

References 107 publications

(146 reference statements)

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“…Five genes, involved in hormone signaling, were up-regulated: ATB11 and ATP-dependent permease (PDR12) (ABA), germination insensitive to ABA mutant 2 (GIM2) (gibberellins), acetyl serotonin O -methyltransferase (ASMT) (salicylic acid), and SPCL51 (brassinosteroids). There is previous evidence that these genes are involved in drought or salinity tolerance/resistance [ 43 , 44 , 45 ]. PDR12 is a PDR-type ABC transporter that mediates cellular uptake of abscisic acid, and mutant experiments demonstrated that this gene facilitates stomata closure and enhances drought tolerance [ 44 ].…”

Section: Discussionmentioning

confidence: 99%

Gaining Insight into Exclusive and Common Transcriptomic Features Linked to Drought and Salinity Responses across Fruit Tree Crops

Benny

Marchese

Giovino

et al. 2020

Plants

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The present study aimed at identifying and mapping key genes expressed in root tissues involved in drought and salinity tolerance/resistance conserved among different fruit tree species. Twenty-six RNA-Seq samples were analyzed from six published studies in five plant species (Olea europaea, Vitis riparia Michx, Prunus mahaleb, Prunus persica, Phoenix dactylifera). This meta-analysis used a bioinformatic pipeline identifying 750 genes that were commonly modulated in three salinity studies and 683 genes that were commonly regulated among three drought studies, implying their conserved role in resistance/tolerance/response to these environmental stresses. A comparison was done on the genes that were in common among both salinity and drought resulted in 82 genes, of which 39 were commonly regulated with the same trend of expression (23 were upregulated and 16 were downregulated). Gene set enrichment and pathway analysis pointed out that pathways encoding regulation of defense response, drug transmembrane transport, and metal ion binding are general key molecular responses to these two abiotic stress responses. Furthermore, hormonal molecular crosstalk plays an essential role in the fine-tuning of plant responses to drought and salinity. Drought and salinity induced a different molecular “hormonal fingerprint”. Dehydration stress specifically enhanced multiple genes responsive to abscisic acid, gibberellin, brassinosteroids, and the ethylene-activated signaling pathway. Salt stress mostly repressed genes encoding for key enzymes in signaling proteins in auxin-, gibberellin-(gibberellin 2 oxidase 8), and abscisic acid-related pathways (aldehyde oxidase 4, abscisic acid-responsive element-binding protein 3). Abiotic stress-related genes were mapped into the chromosome to identify molecular markers usable for the improvement of these complex quantitative traits. This meta-analysis identified genes that serve as potential targets to develop cultivars with enhanced drought and salinity resistance and/or tolerance across different fruit tree crops in a biotechnological sustainable way.

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Section: Discussionmentioning

confidence: 99%

Gaining Insight into Exclusive and Common Transcriptomic Features Linked to Drought and Salinity Responses across Fruit Tree Crops

Benny

Marchese

Giovino

et al. 2020

Plants

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“…Drought tolerance is a quantitative trait controlled by many genes, so the genetic manipulation to generate new cultivars with drought tolerance using the single genetic interventions strategy is difficult [ 111 , 112 ]. However, the understanding of the genetic bases of drought tolerance has increased [ 101 ], and many genes associated with this trait have been identified [ 113 ] and used in the implementation of gene-editing systems [ 114 ], gene silencing [ 115 ] and overexpression methods [ 116 ] to generate materials with possible drought tolerance [ 100 ].…”

Section: Methods and Approaches To Improve Crop Tolerance To Drougmentioning

confidence: 99%

A Dual Strategy of Breeding for Drought Tolerance and Introducing Drought-Tolerant, Underutilized Crops into Production Systems to Enhance Their Resilience to Water Deficiency

Rosero

Granda

Berdugo-Cely

et al. 2020

Plants

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Water scarcity is the primary constraint on crop productivity in arid and semiarid tropical areas suffering from climate alterations; in accordance, agricultural systems have to be optimized. Several concepts and strategies should be considered to improve crop yield and quality, particularly in vulnerable regions where such environmental changes cause a risk of food insecurity. In this work, we review two strategies aiming to increase drought stress tolerance: (i) the use of natural genes that have evolved over time and are preserved in crop wild relatives and landraces for drought tolerance breeding using conventional and molecular methods and (ii) exploiting the reservoir of neglected and underutilized species to identify those that are known to be more drought-tolerant than conventional staple crops while possessing other desired agronomic and nutritive characteristics, as well as introducing them into existing cropping systems to make them more resilient to water deficiency conditions. In the past, the existence of drought tolerance genes in crop wild relatives and landraces was either unknown or difficult to exploit using traditional breeding techniques to secure potential long-term solutions. Today, with the advances in genomics and phenomics, there are a number of new tools available that facilitate the discovery of drought resistance genes in crop wild relatives and landraces and their relatively easy transfer into advanced breeding lines, thus accelerating breeding progress and creating resilient varieties that can withstand prolonged drought periods. Among those tools are marker-assisted selection (MAS), genomic selection (GS), and targeted gene editing (clustered regularly interspaced short palindromic repeat (CRISPR) technology). The integration of these two major strategies, the advances in conventional and molecular breeding for the drought tolerance of conventional staple crops, and the introduction of drought-tolerant neglected and underutilized species into existing production systems has the potential to enhance the resilience of agricultural production under conditions of water scarcity.

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“…The development of tolerant crops by genetic engineering requires the identification of key genetic determinants underlying stress tolerance in plants, and introducing these genes into crops [159,160]. The momentous progress garnered in abiotic stress response research in the model plant Arabidopsis has created an avenue for the identification of drought tolerance conferring genes and the development of transgenics carrying these genes in other crop species.…”

Section: Genetic Engineering Of Drought Tolerant Cereal Cropsmentioning

confidence: 99%

“…Therefore, genetic engineering approach has opened the door to the development of new crop genotypes with improved drought tolerance [103]. Over the past decade, numerous articles and reviews on drought stress tolerant transgenic crop species have been gathered [4,26,[102][103][104][105][106]160]. A selected list of transgenic cereal grain crops, which includes information on transgenes used for the transformations and the resultant drought stress tolerance mechanisms, has already been provided above in Table 2.…”

Section: Genetic Engineering Of Drought Tolerant Cereal Cropsmentioning

confidence: 99%

Adapting Cereal Grain Crops to Drought Stress: 2020 and Beyond

Zenda¹,

Liu²,

Duan³

2021

Abiotic Stress in Plants

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Among other environmental instabilities, drought stress is the primary limitation to cereal crops growth, development and productivity. In the context of continuing global climate change, breeding of drought resistant crop cultivars is the most economical, effective and sustainable strategy for adapting the crop production system and ensuring food security for the growing human population. Additionally, there is need for improving management practices. Whereas conventional breeding has sustained crop productivity gains in the past century, modern technological advancements have revolutionized our identification of important drought tolerance genes and underlying mechanisms, and accelerated new cultivar development. Large-scale high throughput sequencing, phenotyping, 'omics' and systems biology, as well as marker assisted and quantitative trait loci mapping based breeding approaches have offered significant insights into crop drought stress tolerance and provided some new tools for crop improvement. Despite this significant progress in elucidating the mechanisms underlying drought tolerance, considerable challenges remain and our understanding of the crop drought tolerance mechanisms is still abstract. In this chapter, therefore, we highlight current progress in the identification of drought tolerance genes and underlying mechanisms, as well as their practical applications. We then offer a holistic approach for cereal crops adaptation to future climate change exacerbated drought stress.

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Development of Drought-Tolerant Transgenic Wheat: Achievements and Limitations

Cited by 80 publications

References 107 publications

Gaining Insight into Exclusive and Common Transcriptomic Features Linked to Drought and Salinity Responses across Fruit Tree Crops

Gaining Insight into Exclusive and Common Transcriptomic Features Linked to Drought and Salinity Responses across Fruit Tree Crops

A Dual Strategy of Breeding for Drought Tolerance and Introducing Drought-Tolerant, Underutilized Crops into Production Systems to Enhance Their Resilience to Water Deficiency

Adapting Cereal Grain Crops to Drought Stress: 2020 and Beyond

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