Overexpression of the Tomato Pollen Receptor Kinase LePRK1 Rewires Pollen Tube Growth to a Blebbing Mode

Gui, Cai Ping; Dong, Xin; Liu, Haikuan; Huang, Wei; Zhang, Dong; Wang, Shu Jie; Barberini, María Laura; Gao, Xiao Yan; Muschietti, Jorge; McCormick, Sheila; Tang, Wei

doi:10.1105/tpc.114.127381

Cited by 30 publications

(13 citation statements)

References 65 publications

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“…This antagonistic regulation confers a delicate fine-tuning of PRK signaling capacity, thereby transducing different extracellular signals into the pollen tube. Pollen grains over-expressing LePRK1 germinate pollen tubes with enlarged tips, often with "bleb" generating from the tip, which suggests a role of LePRK1 in pollen tube growth (Gui et al, 2014). This finding confirms that the dissociated LePRK1 and LePRK2 both function during pollen tube growth within the pistil, possibly in response to different ligands.…”

Section: Rlks Mediate Pollen Tube Growth In Pistilsupporting

confidence: 76%

“…Interestingly, both LePRK1 and LePRK2 function through KPP (kinase partner protein), a Rop (Rho-like small GTPase from plant) guanine nucleotide exchange factor (RopGEF) (Zhang et al, 2008;Gui et al, 2014). In Arabidopsis, several members of PRK homologs also regulate pollen tube growth through RopGEF (Zhang and McCormick, 2007;Chang et al, 2012).…”

Section: Rlks Mediate Pollen Tube Growth In Pistilmentioning

confidence: 99%

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RLKs orchestrate the signaling in plant male-female interaction

Yang

2016

Sci. China Life Sci.

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Different from animals, sessile plants are equipped with a large receptor-like kinase (RLK) superfamily. RLKs are a family of single trans-membrane proteins with divergent N-terminal extracellular domains capped by a signal peptide and C-terminal intracellular kinase. Researches in the last two decades have uncovered an increasing number of RLKs that regulate plant development, stress response and sexual reproduction, highlighting a dominant role of RLK signaling in cell-to-cell communications. Sexual reproduction in flowering plants is featured by interactions between the male gametophyte and the female tissues to facilitate sperm delivery and fertilization. Emerging evidences suggest that RLKs regulate almost every aspect of plant reproductive process, especially during pollination. Therefore, in this review we will focus mainly on the function and signaling of RLKs in plant male-female interaction and discuss the future prospects on these topics. RLK, signaling, plant reproduction, pollen tube, female gametophyte, male-female interaction, pollen tube growth and guidance, pollen tube reception Citation:Li, H., and Yang, W.C. (2016). RLKs orchestrate the signaling in plant male-female interaction. Sci China Life Sci 59, 867 -877.

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Section: Rlks Mediate Pollen Tube Growth In Pistilsupporting

confidence: 76%

Section: Rlks Mediate Pollen Tube Growth In Pistilmentioning

confidence: 99%

RLKs orchestrate the signaling in plant male-female interaction

Yang

2016

Sci. China Life Sci.

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“…This doubling in growth rate was coupled to a change in apical cell morphology. This morphology and form of growth was reminiscent of blebbing seen in some mutant pollen tubes (Gui et al, 2014). As the apical cell reached a mature size, the very tip ballooned out to form the next cell ( Fig.…”

Section: Microfluidic Chambers Facilitate the Study Of Mutantsmentioning

confidence: 64%

Long-Term Growth of Moss in Microfluidic Devices Enables Subcellular Studies in Development

Bascom

Nelson

et al. 2016

Plant Physiol.

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Key developmental processes that occur on the subcellular and cellular level or occur in occluded tissues are difficult to access, let alone image and analyze. Recently, culturing living samples within polydimethylsiloxane (PDMS) microfluidic devices has facilitated the study of hard-to-reach developmental events. Here, we show that an early diverging land plant, Physcomitrella patens, can be continuously cultured within PDMS microfluidic chambers. Because the PDMS chambers are bonded to a coverslip, it is possible to image P. patens development at high resolution over long time periods. Using PDMS chambers, we report that wild-type protonemal tissue grows at the same rate as previously reported for growth on solid medium. Using longterm imaging, we highlight key developmental events, demonstrate compatibility with high-resolution confocal microscopy, and obtain growth rates for a slow-growing mutant. By coupling the powerful genetic tools available to P. patens with long-term growth and imaging provided by PDMS microfluidic chambers, we demonstrate the capability to study cellular and subcellular developmental events in plants directly and in real time.

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“…For example, pollen overexpressing RAC/ROPs (for RAC-like/ RHOs of plants) show a balloon-shaped tube and growth arrest [35]. Similarly, the overexpression of the tomato pollen-specific LRR-receptor kinases, LePRK1 and LePRK2, led to a permanent formation of blebs at the tip [36] and swollen tips [37], respectively, probably due to the lack of polarity. This phenotype of depolarized pollen tube growth is observed when the pathway of actin depolymerization factor is affected [35].…”

Section: Discussionmentioning

confidence: 99%

“…This phenotype of depolarized pollen tube growth is observed when the pathway of actin depolymerization factor is affected [35]. Pollen tubes overexpressing KPP showed ballooned tips and irregular actin deposition and cytoplasmic streaming as observed by upregulating RAC/ROP signaling [36]. The cytoplasmic domains of LePRK1 and LePRK2 interact with KPP (Kinase Partner Protein) [38], a tomato pollen ROP-GEF that activates ROP in vitro [39].…”

Section: Discussionmentioning

confidence: 99%

Arabidopsis pollen extensins LRX are required for cell wall integrity during pollen tube growth

et al. 2018

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Proper cell wall assembly is crucial during pollen tube growth. Leucine-rich repeat extensins (LRXs) are extracellular glycoproteins which belong to the hydroxyproline-rich glycoprotein (HRGP) family. They contain a conserved N-terminal leucine-rich repeat (LRR) domain and a highly variable C-terminal extensin domain. Here, we characterized four LRX proteins (LRX8 through LRX11) from pollen of Arabidopsis thaliana. To investigate the role of LRX8-LRX11 in pollen germination and pollen tube growth, multiple T-DNA lrx mutants were obtained. The lrx mutants display abnormal pollen tubes with an irregular deposition of callose and pectin. They also show serious alterations in pollen germination and segregation ratio. Our results suggest that LRXs are involved in ensuring proper cell wall assembly during pollen tube growth.

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Overexpression of the Tomato Pollen Receptor Kinase LePRK1 Rewires Pollen Tube Growth to a Blebbing Mode

Cited by 30 publications

References 65 publications

RLKs orchestrate the signaling in plant male-female interaction

RLKs orchestrate the signaling in plant male-female interaction

Long-Term Growth of Moss in Microfluidic Devices Enables Subcellular Studies in Development

Arabidopsis pollen extensins LRX are required for cell wall integrity during pollen tube growth

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