RLKs orchestrate the signaling in plant male-female interaction

Li, Hong-Ju; Yang, Wei‐Cai

doi:10.1007/s11427-016-0118-x

Cited by 28 publications

(27 citation statements)

References 102 publications

(99 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Plant reproduction depends on a series of events, including flower formation, microgametogenesis, megagametogenesis, fertilization of the egg and central cells, and embryogenesis, that are regulated by many different factors, such as transcription factors, epigenetic complexes, small RNAs, and posttranslational protein modifications (for review, see Baroux and Autran, 2015;Gómez et al, 2015;ten Hove et al, 2015;Jiménez-Quesada et al, 2016;Li and Yang, 2016;Sampath and Ephrussi, 2016). A novel protein modifier, SUMO-E3 ligase, also is involved in gametophyte development in Arabidopsis (Arabidopsis thaliana; Ling et al, 2012;Liu et al, 2014) and rice (Oryza sativa; Thangasamy et al, 2011).…”

mentioning

confidence: 99%

Two SUMO Proteases SUMO PROTEASE RELATED TO FERTILITY1 and 2 Are Required for Fertility in Arabidopsis

et al. 2017

View full text Add to dashboard Cite

In plants, the posttranslational modification small ubiquitin-like modifier (SUMO) is involved in regulating several important developmental and cellular processes, including flowering time control and responses to biotic and abiotic stresses. Here, we report two proteases, SUMO PROTEASE RELATED TO FERTILITY1 (SPF1) and SPF2, that regulate male and female gamete and embryo development and remove SUMO from proteins in vitro and in vivo. mutants exhibit abnormal floral structures and embryo development, while mutants exhibit largely a wild-type phenotype. However, double mutants exhibit severe abnormalities in microgametogenesis, megagametogenesis, and embryo development, suggesting that the two genes are functionally redundant. Mutation of and genes also results in misexpression of generative- and embryo-specific genes. In vitro, SPF1 and SPF2 process SUMO1 precursors into a mature form, and as expected in vivo, and mutants accumulate SUMO conjugates. Using a yeast two-hybrid screen, we identified EMBRYO SAC DEVELOPMENT ARREST9 (EDA9) as an SPF1-interacting protein. In vivo, we demonstrate that EDA9 is sumolyated and that, in mutants, EDA9-SUMO conjugates increase in abundance, demonstrating that EDA9 is a substrate of SPF1. Together, our results demonstrate that SPF1 and SPF2 are two SUMO proteases important for plant development in Arabidopsis ().

show abstract

mentioning

confidence: 99%

Two SUMO Proteases SUMO PROTEASE RELATED TO FERTILITY1 and 2 Are Required for Fertility in Arabidopsis

et al. 2017

View full text Add to dashboard Cite

show abstract

“…The spatial distribution of RopGEF activity has been proposed to participate in the restriction of ROP activity at the tube tip to maintain oscillatory tip growth (Li and Yang, ). RopGEFs are positive regulators of ROPs that activate ROPs by promoting the conversion of Rho GTPases from their GDP‐bound inactive form to GTP‐bound active form (Yang, ; Chang et al ., ).…”

Section: Discussionmentioning

confidence: 99%

“…The BUPS1 RLK Is required for normal tip growth of pollen tubes in the pistil Several RLKs have been identified as mediating signalling between the pollen tube and the pistil tissues (Escobar-Restrepo et al, 2007;Dresselhaus and Sprunck, 2012;Chang et al, 2013;Drsselhaus and Franklin-Tong, 2013;Higashiyama and Takeuchi, 2015;Galindo-Trigo et al, 2016;Li and Yang, 2016;Wang et al, 2016;Higashiyama and Yang, 2017), including the CrRLK1L proteins ANX1, ANX2 and FER in Arabidopsis, and Ruptured Pollen tube (RUPO) in rice (Escobar-Restrepo et al, 2007;Boisson-Dernier et al, 2009;Miyazaki et al, 2009;Zou et al, 2011;Liu et al, 2016). Arabidopsis ANXs are required for the integrity of pollen tubes before they enter the embryo sac (Boisson-Dernier et al, 2009;Miyazaki et al, 2009).…”

Section: Discussionmentioning

confidence: 99%

The Arabidopsis CrRLK1L protein kinases BUPS1 and BUPS2 are required for normal growth of pollen tubes in the pistil

et al. 2018

View full text Add to dashboard Cite

In flowering plants, the interaction of pollen tubes with female tissues is important for the accomplishment of double fertilization. Little information is known about the mechanisms that underlie signalling between pollen tubes and female tissues. In this study, two Arabidopsis pollen tube-expressed CrRLK1L protein kinases, Buddha's Paper Seal 1 (BUPS1) and BUPS2, were identified as being required for normal tip growth of pollen tubes in the pistil. They are expressed prolifically in pollen and pollen tubes and are localized on the plasma membrane of the pollen tube tip region. Mutations in BUPS1 drastically reduced seed set. Most of the bups1 mutant pollen tubes growing in the pistil exhibited a swollen pollen tube tip, leading to failure of fertilization. The bups2 pollen tubes had a slightly abnormal morphology but could still accomplish double fertilization. The bups1 bups2 double mutant exhibited a slightly enhanced phenotype compared to the single bups1 mutants. The BUPS1 proteins could form homomers and heteromers with BUPS2, whereas BUPS2 could only form heteromers with BUPS1. The BUPS proteins could interact with the Arabidopsis pollen-expressed RopGEFs in the yeast two-hybrid (Y2H) and bimolecular fluorescence complementation (BiFC) assays. The results indicated that the BUPSs may mediate normal polar growth of pollen tubes in the pistil.

show abstract

“…Proper development of the zygote is dependent on receptor-like kinases, plasma membrane associated kinases and the MAPK signaling pathway, very similar to the early fertilization process 28 . Direct hierarchical interaction between components of the ZAR1-SSP-YDA-MKK4/5-MPK3/6 signaling cascade has been genetically and biochemically revealed, but SSP-YDA interaction was only implicated by genetic analysis.…”

Section: Discussionmentioning

confidence: 99%

The integration of Gβ and MAPK signaling cascade in zygote development

Yuan

Yang

2017

Sci Rep

Self Cite

View full text Add to dashboard Cite

Cells respond to many signals with a limited number of signaling components. Heterotrimeric G proteins and MAPK cascades are universally used by eukaryotic cells to transduce signals in various developmental processes or stress responses by activating different effectors. MAPK cascade is integrated with G proteins by scaffold protein during plant immunity. However, the molecular relationship between G proteins and MAPK modules in plant development is still unclear. In this study, we demonstrate that Arabidopsis Gβ protein AGB1 interacts with MPK3 and 6, MKK4 and 5, as well as the regulatory domains of YODA (YDA), the upstream MEKK of MKK4/5. Remarkably, YDA interacts with the plasma membrane associated SHORT SUSPENSOR (SSP) through its N- and C-terminal region in vitro and in vivo. Additionally, genetic analysis shows that AGB1 functions together with MPK3/6 signaling cascade during the asymmetric division of the zygote. These data indicate that Gβ may function likely as a scaffold, through direct physical interaction with the components of the MPK signaling module in plant development. Our results provide new insights into the molecular functions of G protein and will advance the understanding of the complex mechanism of kinase signaling cascades.

show abstract

RLKs orchestrate the signaling in plant male-female interaction

Cited by 28 publications

References 102 publications

Two SUMO Proteases SUMO PROTEASE RELATED TO FERTILITY1 and 2 Are Required for Fertility in Arabidopsis

Two SUMO Proteases SUMO PROTEASE RELATED TO FERTILITY1 and 2 Are Required for Fertility in Arabidopsis

The Arabidopsis CrRLK1L protein kinases BUPS1 and BUPS2 are required for normal growth of pollen tubes in the pistil

The integration of Gβ and MAPK signaling cascade in zygote development

Contact Info

Product

Resources

About