Overexpression of the soybean transcription factor GmDof4 significantly enhances the lipid content of

Zhang, Jianhui; Hao, Qiang; Bai, Lili; Xu, Jin; Yin, Wei-Bo; Song, Liying; Xu, Limei; Guo, Xuejie; Fan, Chengming; Chen, Yuhong; Ruan, Jue; Hao, Shanting; Li, Yuanguang; Wang, Thomas J.; Hu, Zhongliang

doi:10.1186/preaccept-1189096489110140

Cited by 4 publications

(3 citation statements)

References 33 publications

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“…can grow well under any culture condition (photoautotrophic, mixotrophic, and heterotrophic) with a high lipid content (14 to 63%) [ 35 ], various efforts have been made to use Chlorella spp. for biofuel production [ 13 , 36 ].…”

Section: Discussionmentioning

confidence: 99%

Enhancement of Lipid Production under Heterotrophic Conditions by Overexpression of an Endogenous bZIP Transcription Factor in Chlorella sp. HS2

Lee

Shin

Kim

et al. 2020

J. Microbiol. Biotechnol.

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Transcription factor engineering to regulate multiple genes has shown promise in the field of microalgae genetic engineering. Here, we report the first use of transcription factor engineering in Chlorella sp. HS2, thought to have potential for producing biofuels and bioproducts. We identified seven endogenous bZIP transcription factors in Chlorella sp. HS2 and named them HSbZIP1 through HSbZIP7. We overexpressed HSbZIP1, a C-type bZIP transcription factor, in Chlorella sp. HS2 with the goal of enhancing lipid production. Phenotype screening under heterotrophic conditions showed that all transformants exhibited increased fatty acid production. In particular, HSbZIP1 37 and 58 showed fatty acid methyl ester (FAME) yields of 859 and 1,052 mg/l, respectively, at day 10 of growth under heterotrophic conditions, and these yields were 74% and 113% higher, respectively, than that of WT. To elucidate the mechanism underlying the improved phenotypes, we identified candidate HSbZIP1-regulated genes via transcription factor binding site analysis. We then selected three genes involved in fatty acid synthesis and investigated mRNA expression levels of the genes by qRT-PCR. The result revealed that the possible HSbZIP1-regulated genes involved in fatty acid synthesis were upregulated in the HSbZIP1 transformants. Taken together, our results demonstrate that HSbZIP1 can be utilized to improve lipid production in Chlorella sp. HS2 under heterotrophic conditions.

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Section: Discussionmentioning

confidence: 99%

Enhancement of Lipid Production under Heterotrophic Conditions by Overexpression of an Endogenous bZIP Transcription Factor in Chlorella sp. HS2

Lee

Shin

Kim

et al. 2020

J. Microbiol. Biotechnol.

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“…To better understand the potential of this approach, the study of plants transcription regulators is important in microalgal applications to develop the strategy needed for lipid overproduction. For example, a prominent enhancement in total lipids of Chlorella ellipsoidea was seen after the overexpression of Soybean TF GmDof4, that influence the lipid content in Arabidopsis . Similarly, when Arabidopsis WRINKLED1 transcription factor was over expressed in its vegetative tissues, it caused the hyperaccumulation of FA .…”

Section: Genetic Engineering Approachesmentioning

confidence: 99%

Engineering the metabolic pathways of lipid biosynthesis to develop robust microalgal strains for biodiesel production

Shahid

Rehman

Usman

et al. 2019

Biotech and App Biochem

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Algal lipids have shown promising feedstock to produce biodiesel due to higher energy content, higher cetane number, and renewable nature. However, at present, the lipid productivity is too low to meet the commercial needs. Various approaches can be employed to enhance the lipid content and lipid productivity in microalgae. Stress manipulation is an attractive option to modify the algal lipid content, but it faces the drawback of time‐consuming production processing and lack of information about molecular mechanisms related to triacylglycerides production in response to stress. Developing the robust hyper lipid accumulating algal strains has gained momentum due to advances in metabolic engineering and synthetic biology tools. Understanding the molecular basis of lipid biosynthesis followed by reorienting the related pathways through genomic modification is an alluring strategy that is believed to achieve the industrial and economic robustness. This review portrays the use of integrated OMIC approaches to elucidate the molecular mechanisms of strain adaptability in response to stress conditions, and identification of molecular pathways that should become novel targets to develop novel algal strains. Moreover, an update on the metabolic engineering approaches to improve the lipid production in microalgae is also provided.

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“…For instance, Zhang et al . [ 4 ] increased the lipid content of Chlorella ellipsoidea from 46.4% to 52.9% via transformation and expression of a soybean transcription factor, GmDof4. Similarly, the overexpression of phytoene desaturase in C. zofingiensis resulted in a 32.1% increase in total carotenoids and a 54.1% increase in astaxanthin [ 5 ].…”

Section: Introductionmentioning

confidence: 99%

Isolation and Characterization of a Salt Inducible Promoter from Chlorella vulgaris PKVL7422

Kim

Abdellaoui

et al. 2023

J. Microbiol. Biotechnol.

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Chlorella is a eukaryotic organism that can be used as an industrial host to produce recombinant proteins. In this study, a salt-inducible promoter (SIP) was isolated from the freshwater species Chlorella vulgaris PKVL7422 from the screening of genes that were upregulated after salt treatment. Several cis -acting elements, including stress response elements, were identified in the isolated SIP. Moreover, the Gaussia luciferase gene was cloned after the SIP and transformed into C. vulgaris to test the inducibility of this promoter. Reexamination of transcriptome of C. vulgaris revealed that genes involved in the synthesis of methyl jasmonic acid (MeJA), gibberellin (GA), and abscisic acid (ABA) were upregulated when C. vulgaris was treated with salt. Furthermore, the expression level of recombinant luciferase increased when the transformed C. vulgaris was treated with salt and MeJA, GA, and ABA. This study represents the first report of the C. vulgaris SIP and highlights how transformed microalgae could be used for robust expression of recombinant proteins.

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Overexpression of the soybean transcription factor GmDof4 significantly enhances the lipid content of

Cited by 4 publications

References 33 publications

Enhancement of Lipid Production under Heterotrophic Conditions by Overexpression of an Endogenous bZIP Transcription Factor in Chlorella sp. HS2

Enhancement of Lipid Production under Heterotrophic Conditions by Overexpression of an Endogenous bZIP Transcription Factor in Chlorella sp. HS2

Engineering the metabolic pathways of lipid biosynthesis to develop robust microalgal strains for biodiesel production

Isolation and Characterization of a Salt Inducible Promoter from Chlorella vulgaris PKVL7422

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