Overexpression of the OsChI1 gene, encoding a putative laccase precursor, increases tolerance to drought and salinity stress in transgenic Arabidopsis

Cho, Hyun Yong; Lee, Chanhui; Hwang, Sun‐Goo; Park, Yong Chan; Lim, Hye Lee; Jang, Cheol Seong

doi:10.1016/j.gene.2014.09.018

Cited by 52 publications

(45 citation statements)

References 48 publications

(55 reference statements)

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“…In a previous study, mutations in three laccase genes inhibited Arabidopsis root growth during PEG-induced drought stress [18]. Cho et al reported that overexpression of a rice laccase gene OsChI1 (Os01g61160) enhances salt and drought tolerance in Arabidopsis , which may be associated with the production of some phenolic polymers, and enhances flavonoid oxidation [20]. …”

Section: Discussionmentioning

confidence: 99%

“…However, the roles of lignin biosynthesis-related laccase genes in response to environmental stressors have not been reported. Only AtLAC2 has been shown to be involved in Arabidopsis responses to drought stress [18], as other laccase genes have rarely been reported to be involved in the responses to environmental stress [19,20]. A more comprehensive understanding of this family of laccase genes is needed to better understand the function of laccase.…”

Section: Introductionmentioning

confidence: 99%

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Comprehensive Analysis of Rice Laccase Gene (OsLAC) Family and Ectopic Expression of OsLAC10 Enhances Tolerance to Copper Stress in Arabidopsis

Liu

Luo

Wang

et al. 2017

IJMS

114

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Laccases are encoded by a multigene family and widely distributed in plant genomes where they play roles oxidizing monolignols to produce higher-order lignin involved in plant development and stress responses. We identified 30 laccase genes (OsLACs) from rice, which can be divided into five subfamilies, mostly expressed during early development of the endosperm, growing roots, and stems. OsLACs can be induced by hormones, salt, drought, and heavy metals stresses. The expression level of OsLAC10 increased 1200-fold after treatment with 20 μM Cu for 12 h. The laccase activities of OsLAC10 were confirmed in an Escherichia coli expression system. Lignin accumulation increased in the roots of Arabidopsis over-expressing OsLAC10 (OsLAC10-OX) compared to wild-type controls. After growth on 1/2 Murashige and Skoog (MS) medium containing toxic levels of Cu for seven days, roots of the OsLAC10-OX lines were significantly longer than those of the wild type. Compared to control plants, the Cu concentration decreased significantly in roots of the OsLAC10-OX line under hydroponic conditions. These results provided insights into the evolutionary expansion and functional divergence of OsLAC family. In addition, OsLAC10 is likely involved in lignin biosynthesis, and reduces the uptake of Cu into roots required for Arabidopsis to develop tolerance to Cu.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Comprehensive Analysis of Rice Laccase Gene (OsLAC) Family and Ectopic Expression of OsLAC10 Enhances Tolerance to Copper Stress in Arabidopsis

Liu

Luo

Wang

et al. 2017

IJMS

114

View full text Add to dashboard Cite

show abstract

“…In tomato and maize roots, the transcription of a laccase gene was induced by salt stress (Wei et al, 2000; Liang et al, 2006). Moreover, the overexpression of a putative laccase gene from rice, OsChI1 , increased the tolerance of transgenic Arabidopsis to drought and salinity (Cho et al, 2014). Apart from laccases, peroxidases are another class of enzymes involved in the oxidation of lignin precursors (Zhao et al, 2013) that were upregulated specifically in hvcbp20.ab mutant.…”

Section: Discussionmentioning

confidence: 99%

Mutation in HvCBP20 (Cap Binding Protein 20) Adapts Barley to Drought Stress at Phenotypic and Transcriptomic Levels

et al. 2017

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CBP20 (Cap-Binding Protein 20) encodes a small subunit of the cap-binding complex (CBC), which is involved in the conserved cell processes related to RNA metabolism in plants and, simultaneously, engaged in the signaling network of drought response, which is dependent on ABA. Here, we report the enhanced tolerance to drought stress of barley mutant in the HvCBP20 gene manifested at the morphological, physiological, and transcriptomic levels. Physiological analyses revealed differences between the hvcbp20.ab mutant and its WT in response to a water deficiency. The mutant exhibited a higher relative water content (RWC), a lower stomatal conductance and changed epidermal pattern compared to the WT after drought stress. Transcriptome analysis using the Agilent Barley Microarray integrated with observed phenotypic traits allowed to conclude that the hvcbp20.ab mutant exhibited better fitness to stress conditions by its much more efficient and earlier activation of stress-preventing mechanisms. The network hubs involved in the adjustment of hvcbp20.ab mutant to the drought conditions were proposed. These results enabled to make a significant progress in understanding the role of CBP20 in the drought stress response.

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“…In opposite to fungal laccases, plant laccases participate in lignin synthesis and therefore can be engineered for energy plant improvement (Wang J. et al, 2015). Plant laccases are also involved in pigmentation (Liang et al, 2006) or pigment breakdown (Fang F. et al, 2015), root elongation (Liang et al, 2006), and responses to external stresses (Cho et al, 2014; Kim et al, 2014). …”

Section: Natural Laccase Producersmentioning

confidence: 99%

Laccases: Production, Expression Regulation, and Applications in Pharmaceutical Biodegradation

et al. 2017

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Laccases are a family of copper-containing oxidases with important applications in bioremediation and other various industrial and biotechnological areas. There have been over two dozen reviews on laccases since 2010 covering various aspects of this group of versatile enzymes, from their occurrence, biochemical properties, and expression to immobilization and applications. This review is not intended to be all-encompassing; instead, we highlighted some of the latest developments in basic and applied laccase research with an emphasis on laccase-mediated bioremediation of pharmaceuticals, especially antibiotics. Pharmaceuticals are a broad class of emerging organic contaminants that are recalcitrant and prevalent. The recent surge in the relevant literature justifies a short review on the topic. Since low laccase yields in natural and genetically modified hosts constitute a bottleneck to industrial-scale applications, we also accentuated a genus of laccase-producing white-rot fungi, Cerrena, and included a discussion with regards to regulation of laccase expression.

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Overexpression of the OsChI1 gene, encoding a putative laccase precursor, increases tolerance to drought and salinity stress in transgenic Arabidopsis

Cited by 52 publications

References 48 publications

Comprehensive Analysis of Rice Laccase Gene (OsLAC) Family and Ectopic Expression of OsLAC10 Enhances Tolerance to Copper Stress in Arabidopsis

Comprehensive Analysis of Rice Laccase Gene (OsLAC) Family and Ectopic Expression of OsLAC10 Enhances Tolerance to Copper Stress in Arabidopsis

Mutation in HvCBP20 (Cap Binding Protein 20) Adapts Barley to Drought Stress at Phenotypic and Transcriptomic Levels

Laccases: Production, Expression Regulation, and Applications in Pharmaceutical Biodegradation

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